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An Elongin-Cullin-SOCS Box Complex Regulates Stress-Induced Serotonergic Neuromodulation.

Gracida X, Dion MF, Harris G, Zhang Y, Calarco JA Cell Rep 2017 Dec 12; 21(11):3089-3101 PMID: 29241538 Abstract Neuromodulatory cells transduce environmental information into long-lasting behavioral responses. However, the mechanisms governing how neuronal cells influence behavioral plasticity are difficult to characterize. Here, we adapted the translating ribosome affinity purification (TRAP) approach in C. elegans to profile … Read More

CRISPR-mediated genetic interaction profiling identifies RNA binding proteins controlling metazoan fitness.

Norris AD, Gracida X, Calarco JA Elife 2017 07 18; 6(): PMID: 28718764 Abstract Genetic interaction screens have aided our understanding of complex genetic traits, diseases, and biological pathways. However, approaches for synthetic genetic analysis with null-alleles in metazoans have not been feasible. Here, we present a CRISPR/Cas9-based Synthetic Genetic Interaction (CRISPR-SGI) approach enabling systematic … Read More

Cell type-specific transcriptome profiling in C. elegans using the Translating Ribosome Affinity Purification technique.

Gracida X, Calarco JA Methods 2017 08 15; 126():130-137 PMID: 28648677 Abstract Organs and specific cell types execute specialized functions in multicellular organisms, in large part through customized gene expression signatures. Thus, profiling the transcriptomes of specific cell and tissue types remains an important tool for understanding how cells become specialized. Methodological approaches to detect … Read More