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Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System.

Calarco JA, Friedland AE Methods Mol. Biol. 2015; 1327():59-74 PMID: 26423968 Abstract The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective … Read More

Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes.

Norris AD, Kim HM, Colaiácovo MP, Calarco JA Genetics 2015 Oct; 201(2):449-58 PMID: 26232410 Abstract Use of the CRISPR/Cas9 RNA-guided endonuclease complex has recently enabled the generation of double-strand breaks virtually anywhere in the C. elegans genome. Here, we present an improved strategy that makes all steps in the genome editing process more efficient. We … Read More

A pair of RNA-binding proteins controls networks of splicing events contributing to specialization of neural cell types.

Norris AD, Gao S, Norris ML, Ray D, Ramani AK, Fraser AG, Morris Q, Hughes TR, Zhen M, Calarco JA Mol. Cell 2014 Jun 19; 54(6):946-59 PMID: 24910101 Abstract Alternative splicing is important for the development and function of the nervous system, but little is known about the differences in alternative splicing between distinct types … Read More