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Application of time-of-flight-secondary ion mass spectrometry for the detection of enzyme activity on solid wood substrates

Goacher RE, Edwards EA, Yakunin AF, Mims CA, Master ER Anal. Chem. 2012 May;84(10):4443-51 PMID: 22507179 Abstract Time-of-flight-secondary ion mass spectrometry (TOF-SIMS) is a surface analysis technique that is herein demonstrated to be a viable tool for the detection of enzyme activity on solid substrates. Proof-of-principle experiments are presented that utilize commercial cellulase and laccase … Read More

The embryonic leaf identity gene FUSCA3 regulates vegetative phase transitions by negatively modulating ethylene-regulated gene expression in Arabidopsis

Lumba S, Tsuchiya Y, Delmas F, Hezky J, Provart NJ, Shi Lu Q, McCourt P, Gazzarrini S BMC Biol. 2012;10:8 PMID: 22348746 Abstract BACKGROUND: The embryonic temporal regulator FUSCA3 (FUS3) plays major roles in the establishment of embryonic leaf identity and the regulation of developmental timing. Loss-of-function mutations of this B3 domain transcription factor result … Read More

A bacterial acetyltransferase destroys plant microtubule networks and blocks secretion

Lee AH, Hurley B, Felsensteiner C, Yea C, Ckurshumova W, Bartetzko V, Wang PW, Quach V, Lewis JD, Liu YC, Börnke F, Angers S, Wilde A, Guttman DS, Desveaux D PLoS Pathog. 2012 Feb;8(2):e1002523 PMID: 22319451 Abstract The eukaryotic cytoskeleton is essential for structural support and intracellular transport, and is therefore a common target of … Read More