Adriano Senatore

faculty_img Academic Title: Assistant Professor

Campus: UTM

CSB Appointment: Full

Primary Undergraduate Department:
Biology

Graduate Programs:
Cell & Systems Biology

Titles and Honors:

Academic or Administrative Appointments:

Education:
Ph.D. University of Waterloo 2012
M.Sc. University of Guelph 2006

 

Mailing Address
Department of Biology
University of Toronto
3359 Mississauga Road North
Mississauga, ON L5L 1C6
Canada

 

Contact Information
Office phone:
Office: DV 3033
Lab:
Lab phone:
Email: adriano.senatore@utoronto.ca
URL: http://www.utm.utoronto.ca/biology/people/senatore-adriano

 

Research Areas
Animal Biology
Bioinformatics & Computational Biology

Cell Biology
Evolutionary Biology
Functional Genomics
Neurobiology
Physiology
Proteomics

 

Research

The long-term goal of my research is to better understand the molecular underpinnings of synaptic evolution and diversity. To achieve this, we compare what we know about synaptic physiology and structure in mammalian and invertebrate model organisms, with two of the most early-diverging animals: 1) Placozoan Trichoplax adhaerens, a simple marine organism that exhibits motile behaviors including feeding, chemotaxis and phototaxis, yet lacks neurons, muscle and synapses; and 2) the comb jelly Mnemiopsis leidyi, from the most basal animal phylum Ctenophora, which appears to have independently-evolved synapses. We focus on 1) voltage-gated calcium (Cav) channels, which in humans and invertebrates play crucial roles in synaptic transmission, and on 2) synaptic scaffolding proteins, which play dynamic roles in synapse formation and homeostasis. My team and I are initiating comparative structure-function and physiology studies of Cav channels, comparing human and other invertebrate channels with highly divergent orthologues/homologues from Trichoplax and Mnemiopsis. Here, objectives are to gain evolutionary insights into the biophysical and pharmacological features of each Cav channel type, and to determine whether the roles that Cav channels play in Trichoplax and Mnemiopsis resemble known synaptic functions of Cav channels in other well-studied organisms. We are also applying high-throughput proteomics to evaluate if key protein-protein interactions that underlie synaptic scaffolding, including the association of Cav channels with pre-synaptic exocytotic machinery, are absent/present in Trichoplax, in accordance with its lack of synapses, and to define the molecular architecture of the independently-evolved and poorly understood Mnemiopsis synapse.

 

Publications

2015

Top
Identification of genes related to learning and memory in the brain transcriptome of the mollusc, Hermissenda crassicornis.Tamvacakis AN, Senatore A, Katz PS.Learn. Mem. 2015 Dec;22(12):617-21
pubmed
Deep mRNA sequencing of the Tritonia diomedea brain transcriptome provides access to gene homologues for neuronal excitability, synaptic transmission and peptidergic signalling.Senatore A, Edirisinghe N, Katz PS.PLoS ONE 2015;10(2):e0118321
pubmed

2014

Top
Gd3+ and calcium sensitive, sodium leak currents are features of weak membrane-glass seals in patch clamp recordings.Boone AN, Senatore A, Chemin J, Monteil A, Spafford JD.PLoS ONE 2014;9(6):e98808
pubmed
T-type channels become highly permeable to sodium ions using an alternative extracellular turret region (S5-P) outside the selectivity filter.Senatore A, Guan W, Boone AN, Spafford JD.J. Biol. Chem. 2014 Apr;289(17):11952-69
pubmed
Cav3 T-type channels: regulators for gating, membrane expression, and cation selectivity.Senatore A, Guan W, Spafford JD.Pflugers Arch. 2014 Apr;466(4):645-60
pubmed

2013

Top
A uniquely adaptable pore is consistent with NALCN being an ion sensor.Senatore A, Spafford JD.Channels (Austin) 2013 Mar-Apr;7(2):60-8
pubmed
NALCN ion channels have alternative selectivity filters resembling calcium channels or sodium channels.Senatore A, Monteil A, van Minnen J, Smit AB, Spafford JD.PLoS ONE 2013;8(1):e55088
pubmed
Induction of a ricinosomal-protease and programmed cell death in tomato endosperm by gibberellic acid.Trobacher CP, Senatore A, Holley C, Greenwood JS.Planta 2013 Mar;237(3):665-79
pubmed

2012

Top
Gene transcription and splicing of T-type channels are evolutionarily-conserved strategies for regulating channel expression and gating.Senatore A, Spafford JD.PLoS ONE 2012;7(6):e37409
pubmed

2011

Top
Mapping of dihydropyridine binding residues in a less sensitive invertebrate L-type calcium channel (LCa v 1).Senatore A, Boone A, Lam S, Dawson TF, Zhorov B, Spafford JD.Channels (Austin) 2011 Mar-Apr;5(2):173-87
pubmed
Optimized transfection strategy for expression and electrophysiological recording of recombinant voltage-gated ion channels in HEK-293T cells.Senatore A, Boone AN, Spafford JD.J Vis Exp 2011;(47)
pubmed

2010

Top
G-proteins modulate invertebrate synaptic calcium channel (LCav2) differently from the classical voltage-dependent regulation of mammalian Cav2.1 and Cav2.2 channels.Huang X, Senatore A, Dawson TF, Quan Q, Spafford JD.J. Exp. Biol. 2010 Jun;213(Pt 12):2094-103
pubmed
Transient and big are key features of an invertebrate T-type channel (LCav3) from the central nervous system of Lymnaea stagnalis.Senatore A, Spafford JD.J. Biol. Chem. 2010 Mar;285(10):7447-58
pubmed

2009

Top
Ricinosomes predict programmed cell death leading to anther dehiscence in tomato.Senatore A, Trobacher CP, Greenwood JS.Plant Physiol. 2009 Feb;149(2):775-90
pubmed

2008

Top
PKC-induced intracellular trafficking of Ca(V)2 precedes its rapid recruitment to the plasma membrane.Zhang Y, Helm JS, Senatore A, Spafford JD, Kaczmarek LK, Jonas EA.J. Neurosci. 2008 Mar;28(10):2601-12
pubmed