Daphne Goring

faculty_img Academic Title: Professor

Campus: St. George

CSB Appointment: Full

Primary Undergraduate Department:
Cell & Systems Biology

Graduate Programs:
Cell & Systems Biology

Titles and Honors:
Fellow of the American Association for the Advancement of Science

Academic or Administrative Appointments
Education:
Ph.D. University of Toronto 1990
M.Sc, McGill University 1985
B.Sc. Trent University 1983

 

Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Willcocks St.
Toronto, ON M5S 3B2
Canada

 

Contact Information
Office phone: 416-978-2378
Office: ESC 4029
Lab: ESC 4027
Lab phone: 416-978-8158
Email: d.goring@utoronto.ca
URL: http://labs.csb.utoronto.ca/goring

 

Research Areas
Cell Biology
Molecular Biology
Plant Biology

 

Research

One area that my research group investigates is the signalling systems regulating compatible and self-incompatible pollen responses in the pistil. These are critical recognition systems for selecting which pollen grains will be accepted or rejected, and thus, selecting optimal pollen grains for fertilization. My research is focused on the complex intracellular signalling takes place in the stigma to identify and accept compatible pollen while rejecting self-incompatible pollen and ignoring foreign pollen. In a search for factors that act early in the compatible pollen responses, my research group identified the Exo70A1 exocyst subunit as a critical component in the stigma to promote pollen hydration and pollen tube growth and are currently exploring this model in more detail. In contrast to the compatible pollen recognition system is the self-incompatibility system which prevents self-fertilization and inbreeding. In the self-incompatibility response, a stigma-specific S Receptor Kinase (SRK) is responsible for binding the SP11/SCR ligand from the self-incompatible pollen and activates a signal transduction pathway in the stigmatic papilla to reject this pollen. My research group identified three proteins which are involved in this signalling pathway, THL1, THL2 and ARC1. THL1 and THL2 belong to the thioredoxin h family and act as negative regulators of SRK. ARC1 is an E3 ubiquitin ligase which is activated downstream of SRK and is proposed to inhibit compatibility factors such as Exo70A1 causing pollen rejection. My research is currently exploring in more detail how these events leads to pollen rejection.

 

Publications

2016

Top

Pollen Acceptance or Rejection: A Tale of Two Pathways.Doucet, Jennifer, Hyun Kyung Lee, and Daphne R Goring. 2016. Trends in plant science, no. 12 (November 11).
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Dominance modifier: Expanding mate options.Goring, Daphne R. 2016. Nature plants (December 22).
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Following the Time-Course of Post-pollination Events by Transmission Electron Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible Pollinations.Safavian, Darya, Jennifer Doucet, and Daphne R Goring. 2016. Methods in molecular biology (Clifton, N.J.)
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Yeast two-hybrid interactions between Arabidopsis lyrata S Receptor Kinase and the ARC1 E3 ligase.Indriolo E, Goring DR.Plant Signal Behav 2016 May;
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2015

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RNA Silencing of Exocyst Genes in the Stigma Impairs the Acceptance of Compatible Pollen in Arabidopsis.Safavian D, Zayed Y, Indriolo E, Chapman L, Ahmed A, Goring DR.Plant Physiol. 2015 Dec;169(4):2526-38
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PERK-KIPK-KCBP signalling negatively regulates root growth in Arabidopsis thaliana.Humphrey TV, Haasen KE, Aldea-Brydges MG, Sun H, Zayed Y, Indriolo E, Goring DR.J. Exp. Bot. 2015 Jan;66(1):71-83
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2014

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Autophagy in the rejection of self-pollen in the mustard family.Safavian D, Goring D.Autophagy 2014;10(12):2379-80
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Reversible ubiquitylation in plant biology.Fu H, Goring DR, Genschik P.Front Plant Sci 2014;5:707
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The ARC1 E3 ligase promotes a strong and stable self-incompatibility response in Arabidopsis species: response to the Nasrallah and Nasrallah commentary.Goring DR, Indriolo E, Samuel MA.Plant Cell 2014 Oct;26(10):3842-6
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High humidity partially rescues the Arabidopsis thaliana exo70A1 stigmatic defect for accepting compatible pollen.Safavian D, Jamshed M, Sankaranarayanan S, Indriolo E, Samuel MA, Goring DR.Plant Reprod 2014 Sep;27(3):121-7
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A conserved role for the ARC1 E3 ligase in Brassicaceae self-incompatibility.Indriolo E, Goring DR.Front Plant Sci 2014;5:181
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The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in Arabidopsis.Indriolo E, Safavian D, Goring DR.Plant Cell 2014 Apr;26(4):1525-1543
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2013

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Secretory activity is rapidly induced in stigmatic papillae by compatible pollen, but inhibited for self-incompatible pollen in the Brassicaceae.Safavian D, Goring DR.PLoS ONE 2013;8(12):e84286
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2012

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The ARC1 E3 ligase gene is frequently deleted in self-compatible Brassicaceae species and has a conserved role in Arabidopsis lyrata self-pollen rejection.Indriolo E, Tharmapalan P, Wright SI, Goring DR.Plant Cell 2012 Nov;24(11):4607-20
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2011

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Proteomic analysis of Brassica stigmatic proteins following the self-incompatibility reaction reveals a role for microtubule dynamics during pollen responses.Samuel MA, Tang W, Jamshed M, Northey J, Patel D, Smith D, Siu KW, Muench DG, Wang ZY, Goring DR.Mol. Cell Proteomics 2011 Dec;10(12):M111.011338
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Altered germination and subcellular localization patterns for PUB44/SAUL1 in response to stress and phytohormone treatments.Salt JN, Yoshioka K, Moeder W, Goring DR.PLoS ONE 2011;6(6):e21321
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Misregulation of phosphoinositides in Arabidopsis thaliana decreases pollen hydration and maternal fertility.Chapman LA, Goring DR.Sex. Plant Reprod. 2011 Dec;24(4):319-26
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2010

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Plant science. Pollen gets more complex.Indriolo E, Goring DR.Science 2010 Nov;330(6005):767-8
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Gene expression: How plants avoid incest.Goring D, Indriolo E.Nature 2010 Aug;466(7309):926-8
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Pollen-pistil interactions regulating successful fertilization in the Brassicaceae.Chapman LA, Goring DR.J. Exp. Bot. 2010 Apr;61(7):1987-99
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Characterization of the Arabidopsis thaliana exocyst complex gene families by phylogenetic, expression profiling, and subcellular localization studies.Chong YT, Gidda SK, Sanford C, Parkinson J, Mullen RT, Goring DR.New Phytol. 2010 Jan;185(2):401-19
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2009

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Cellular pathways regulating responses to compatible and self-incompatible pollen in Brassica and Arabidopsis stigmas intersect at Exo70A1, a putative component of the exocyst complex.Samuel MA, Chong YT, Haasen KE, Aldea-Brydges MG, Stone SL, Goring DR.Plant Cell 2009 Sep;21(9):2655-71
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The diversity of plant U-box E3 ubiquitin ligases: from upstream activators to downstream target substrates.Yee D, Goring DR.J. Exp. Bot. 2009;60(4):1109-21
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2008

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Interactions between the S-domain receptor kinases and AtPUB-ARM E3 ubiquitin ligases suggest a conserved signaling pathway in Arabidopsis.Samuel MA, Mudgil Y, Salt JN, Delmas F, Ramachandran S, Chilelli A, Goring DR.Plant Physiol. 2008 Aug;147(4):2084-95
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Self/nonself perception and recognition mechanisms in plants: a comparison of self-incompatibility and innate immunity.Sanabria N, Goring D, N├╝rnberger T, Dubery I.New Phytol. 2008;178(3):503-14
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2007

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Sentinels at the wall: cell wall receptors and sensors.Humphrey TV, Bonetta DT, Goring DR.New Phytol. 2007;176(1):7-21
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2006

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Altered Expression of PERK Receptor Kinases in Arabidopsis Leads to Changes in Growth and Floral Organ Formation.Haffani YZ, Silva-Gagliardi NF, Sewter SK, Grace Aldea M, Zhao Z, Nakhamchik A, Cameron RK, Goring DR.Plant Signal Behav 2006 Sep;1(5):251-60
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Multifunctional arm repeat domains in plants.Samuel MA, Salt JN, Shiu SH, Goring DR.Int. Rev. Cytol. 2006;253:1-26
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2004

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A comprehensive expression analysis of the Arabidopsis proline-rich extensin-like receptor kinase gene family using bioinformatic and experimental approaches.Nakhamchik A, Zhao Z, Provart NJ, Shiu SH, Keatley SK, Cameron RK, Goring DR.Plant Cell Physiol. 2004 Dec;45(12):1875-81
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Antisense suppression of thioredoxin h mRNA in Brassica napus cv. Westar pistils causes a low level constitutive pollen rejection response.Haffani YZ, Gaude T, Cock JM, Goring DR.Plant Mol. Biol. 2004 Jul;55(5):619-30
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Plant sciences. Self-rejection–a new kinase connection.Goring DR, Walker JC.Science 2004 Mar;303(5663):1474-5
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A large complement of the predicted Arabidopsis ARM repeat proteins are members of the U-box E3 ubiquitin ligase family.Mudgil Y, Shiu SH, Stone SL, Salt JN, Goring DR.Plant Physiol. 2004 Jan;134(1):59-66
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2003

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ARC1 is an E3 ubiquitin ligase and promotes the ubiquitination of proteins during the rejection of self-incompatible Brassica pollen.Stone SL, Anderson EM, Mullen RT, Goring DR.Plant Cell 2003 Apr;15(4):885-98
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2002

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The proline-rich, extensin-like receptor kinase-1 (PERK1) gene is rapidly induced by wounding.Silva NF, Goring DR.Plant Mol. Biol. 2002 Nov;50(4-5):667-85
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