Elizabeth Edwards

faculty_img Academic Title: Professor

Campus: St. George

CSB Appointment: Cross Appointment

Primary Undergraduate Department:
Chemical Engineering & Applied Chemistry

Graduate Programs:
Cell & Systems Biology
Genome Biology & Bioinformatics

Titles and Honors:
Awards
Canada Research Chair (tier 1) in Anaerobic Biotechnology, 2014
Fellow, Royal Society of Canada, 2012
Fellow, Canadian Academy of Engineering 2011
Fellow, AAAS 2011
Killam Research Fellowship, 2008-2010

Memberships
Professional Engineers of Ontario (PEO)
Association of Environmental Engineering and Science Professors (AEESP)
American Society for Microbiology (ASM)
American Chemical Society (ACS)

Academic or Administrative Appointments:
Director, BioZone Centre for Applied Bioscience and Bioengineering Research

Education:
PhD Stanford 1993 M. Eng. McGill 1985 B. Eng. McGill 1983

 

Mailing Address
Department of Chemical Engineering & Applied Chemistry
University of Toronto
200 College St.
Toronto, ON M5S 3E5
Canada

 

Contact Information
Office phone: 416-946-3506
Office: WB 420D
Lab: WB 311
Lab phone: 416-978-4222
Email: elizabeth.edwards@utoronto.ca
URL: http://www.labs.chem-eng.utoronto.ca/edwards/

 

Research Areas
Bioinformatics & Computational Biology
Biotechnology
Cell Biology
Comparative Genomics
Functional Genomics
Metabolomics
Microbiology
Proteomics

 

Research

RESEARCH INTERESTS Biodegradation and bioremediation of organic compounds in groundwater, anaerobic microbial biotransformation reactions, isotope fractionation, environmental biotechnology, environmental microbiology, anaerobic digestion, wastewater treatment, molecular microbiology and ecology, endocrine disrupting compounds, microarray and sequencing technology, environmental genomics, metagenomics, metaproteomics, bioaugmentation, site remediation, groundwater.

 

Publications

2014

Top
Metatranscriptome of an anaerobic benzene-degrading, nitrate-reducing enrichment culture reveals involvement of carboxylation in benzene ring activation.Luo F, Gitiafroz R, Devine CE, Gong Y, Hug LA, Raskin L, Edwards EA.Appl. Environ. Microbiol. 2014 Jul;80(14):4095-107
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New insights into Dehalococcoides mccartyi metabolism from a reconstructed metabolic network-based systems-level analysis of D. mccartyi transcriptomes.Islam MA, Waller AS, Hug LA, Provart NJ, Edwards EA, Mahadevan R.PLoS ONE 2014;9(4):e94808
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Bioremediation of chlorinated ethenes in fractured bedrock and associated changes in dechlorinating and nondechlorinating microbial populations.Pérez-de-Mora A, Zila A, McMaster ML, Edwards EA.Environ. Sci. Technol. 2014 May;48(10):5770-9
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Bioaugmentation with distinct Dehalobacter strains achieves chloroform detoxification in microcosms.Justicia-Leon SD, Higgins S, Mack EE, Griffiths DR, Tang S, Edwards EA, Löffler FE.Environ. Sci. Technol. 2014 Feb;48(3):1851-8
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2013

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Complete Genome Sequence of Bacteroidales Strain CF from a Chloroform-Dechlorinating Enrichment Culture.Tang S, Edwards EA.Genome Announc 2013;1(6)
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Diversity of reductive dehalogenase genes from environmental samples and enrichment cultures identified with degenerate primer PCR screens.Hug LA, Edwards EA.Front Microbiol 2013;4:341
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Meeting report: 1st international functional metagenomics workshop may 7-8, 2012, st. Jacobs, ontario, Canada.Engel K, Ashby D, Brady SF, Cowan DA, Doemer J, Edwards EA, Fiebig K, Martens EC, McCormac D, Mead DA, Miyazaki K, Moreno-Hagelsieb G, O'Gara F, Reid A, Rose DR, Simonet P, Sjöling S, Smalla K, Streit WR, Tedman-Jones J, Valla S, Wellington EM, Wu CC, Liles MR, Neufeld JD, Sessitsch A, Charles TC.Stand Genomic Sci 2013 Apr;8(1):106-11
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Overview of organohalide-respiring bacteria and a proposal for a classification system for reductive dehalogenases.Hug LA, Maphosa F, Leys D, Löffler FE, Smidt H, Edwards EA, Adrian L.Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2013 Apr;368(1616):20120322
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Identification of Dehalobacter reductive dehalogenases that catalyse dechlorination of chloroform, 1,1,1-trichloroethane and 1,1-dichloroethane.Tang S, Edwards EA.Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2013 Apr;368(1616):20120318
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Anaerobic conversion of chlorobenzene and benzene to CH4 and CO2 in bioaugmented microcosms.Liang X, Devine CE, Nelson J, Sherwood Lollar B, Zinder S, Edwards EA.Environ. Sci. Technol. 2013 Mar;47(5):2378-85
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Paediatric home ventilatory support: changing milieu, proactive solutions.Edwards EA, Nixon GM, .J Paediatr Child Health 2013 Jan;49(1):13-8
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Functional characterization of reductive dehalogenases by using blue native polyacrylamide gel electrophoresis.Tang S, Chan WW, Fletcher KE, Seifert J, Liang X, Löffler FE, Edwards EA, Adrian L.Appl. Environ. Microbiol. 2013 Feb;79(3):974-81
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Dehalococcoides mccartyi gen. nov., sp. nov., obligately organohalide-respiring anaerobic bacteria relevant to halogen cycling and bioremediation, belong to a novel bacterial class, Dehalococcoidia classis nov., order Dehalococcoidales ord. nov. and family Dehalococcoidaceae fam. nov., within the phylum Chloroflexi.Löffler FE, Yan J, Ritalahti KM, Adrian L, Edwards EA, Konstantinidis KT, Müller JA, Fullerton H, Zinder SH, Spormann AM.Int. J. Syst. Evol. Microbiol. 2013 Feb;63(Pt 2):625-35
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2012

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Semi-automatic in silico gap closure enabled de novo assembly of two Dehalobacter genomes from metagenomic data.Tang S, Gong Y, Edwards EA.PLoS ONE 2012;7(12):e52038
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Large carbon isotope fractionation during biodegradation of chloroform by Dehalobacter cultures.Chan CC, Mundle SO, Eckert T, Liang X, Tang S, Lacrampe-Couloume G, Edwards EA, Lollar BS.Environ. Sci. Technol. 2012 Sep;46(18):10154-60
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Comparative metagenomics of three Dehalococcoides-containing enrichment cultures: the role of the non-dechlorinating community.Hug LA, Beiko RG, Rowe AR, Richardson RE, Edwards EA.BMC Genomics 2012;13:327
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Discovery of a trans-dichloroethene-respiring Dehalogenimonas species in the 1,1,2,2-tetrachloroethane-dechlorinating WBC-2 consortium.Manchester MJ, Hug LA, Zarek M, Zila A, Edwards EA.Appl. Environ. Microbiol. 2012 Aug;78(15):5280-7
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Genomic determinants of organohalide-respiration in Geobacter lovleyi, an unusual member of the Geobacteraceae.Wagner DD, Hug LA, Hatt JK, Spitzmiller MR, Padilla-Crespo E, Ritalahti KM, Edwards EA, Konstantinidis KT, Löffler FE.BMC Genomics 2012;13:200
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Application of time-of-flight-secondary ion mass spectrometry for the detection of enzyme activity on solid wood substrates.Goacher RE, Edwards EA, Yakunin AF, Mims CA, Master ER.Anal. Chem. 2012 May;84(10):4443-51
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Monitoring biodegradation of ethene and bioremediation of chlorinated ethenes at a contaminated site using compound-specific isotope analysis (CSIA).Mundle SO, Johnson T, Lacrampe-Couloume G, Pérez-de-Mora A, Duhamel M, Edwards EA, McMaster ML, Cox E, Révész K, Sherwood Lollar B.Environ. Sci. Technol. 2012 Feb;46(3):1731-8
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Transcriptional analysis of a Dehalococcoides-containing microbial consortium reveals prophage activation.Waller AS, Hug LA, Mo K, Radford DR, Maxwell KL, Edwards EA.Appl. Environ. Microbiol. 2012 Feb;78(4):1178-86
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2011

Top
Quantifying the effects of 1,1,1-trichloroethane and 1,1-dichloroethane on chlorinated ethene reductive dehalogenases.Chan WW, Grostern A, Löffler FE, Edwards EA.Environ. Sci. Technol. 2011 Nov;45(22):9693-702
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Pathway-dependent isotope fractionation during aerobic and anaerobic degradation of monochlorobenzene and 1,2,4-trichlorobenzene.Liang X, Howlett MR, Nelson JL, Grant G, Dworatzek S, Lacrampe-Couloume G, Zinder SH, Edwards EA, Sherwood Lollar B.Environ. Sci. Technol. 2011 Oct;45(19):8321-7
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Design and verification of a pangenome microarray oligonucleotide probe set for Dehalococcoides spp.Hug LA, Salehi M, Nuin P, Tillier ER, Edwards EA.Appl. Environ. Microbiol. 2011 Aug;77(15):5361-9
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Site-specific mobilization of vinyl chloride respiration islands by a mechanism common in Dehalococcoides.McMurdie PJ, Hug LA, Edwards EA, Holmes S, Spormann AM.BMC Genomics 2011;12:287
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Mapping the reaction coordinates of enzymatic defluorination.Chan PW, Yakunin AF, Edwards EA, Pai EF.J. Am. Chem. Soc. 2011 May;133(19):7461-8
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Do New Zealand children with non-cystic fibrosis bronchiectasis show disease progression?.Munro KA, Reed PW, Joyce H, Perry D, Twiss J, Byrnes CA, Edwards EA.Pediatr. Pulmonol. 2011 Feb;46(2):131-8
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2010

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Sequence- and activity-based screening of microbial genomes for novel dehalogenases.Chan WY, Wong M, Guthrie J, Savchenko AV, Yakunin AF, Pai EF, Edwards EA.Microb Biotechnol 2010 Jan;3(1):107-20
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Insights into enzyme kinetics of chloroethane biodegradation using compound specific stable isotopes.Lollar BS, Hirschorn S, Mundle SO, Grostern A, Edwards EA, Lacrampe-Couloume G.Environ. Sci. Technol. 2010 Oct;44(19):7498-503
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Characterizing the metabolism of Dehalococcoides with a constraint-based model.Ahsanul Islam M, Edwards EA, Mahadevan R.PLoS Comput. Biol. 2010;6(8)
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Chloroform respiration to dichloromethane by a Dehalobacter population.Grostern A, Duhamel M, Dworatzek S, Edwards EA.Environ. Microbiol. 2010 Apr;12(4):1053-60
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Resolution of sleep-disordered breathing in a dialysis-dependent child post-renal transplantation.Ball E, Kara T, McNamara D, Edwards EA.Pediatr. Nephrol. 2010 Jan;25(1):173-7
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2009

Top
Planktonic cell yield is linked to biofilm development.Bester E, Edwards EA, Wolfaardt GM.Can. J. Microbiol. 2009 Oct;55(10):1195-206
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1,1,1-trichloroethane and 1,1-dichloroethane reductive dechlorination kinetics and co-contaminant effects in a Dehalobacter-containing mixed culture.Grostern A, Chan WW, Edwards EA.Environ. Sci. Technol. 2009 Sep;43(17):6799-807
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Characterization of a Dehalobacter coculture that dechlorinates 1,2-dichloroethane to ethene and identification of the putative reductive dehalogenase gene.Grostern A, Edwards EA.Appl. Environ. Microbiol. 2009 May;75(9):2684-93
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2008

Top
Isotopic evidence suggests different initial reaction mechanisms for anaerobic benzene biodegradation.Mancini SA, Devine CE, Elsner M, Nandi ME, Ulrich AC, Edwards EA, Lollar BS.Environ. Sci. Technol. 2008 Nov;42(22):8290-6
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Disruption of rosetting in Plasmodium falciparum malaria with chemically modified heparin and low molecular weight derivatives possessing reduced anticoagulant and other serine protease inhibition activities.Skidmore MA, Dumax-Vorzet AF, Guimond SE, Rudd TR, Edwards EA, Turnbull JE, Craig AG, Yates EA.J. Med. Chem. 2008 Mar;51(5):1453-8
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2007

Top
A future for the protein structure initiative.Edwards AM, Edwards EA.Structure 2007 Dec;15(12):1525-6
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Quantification of biotransformation of chlorinated hydrocarbons in a biostimulation study: added value via stable carbon isotope analysis.Hirschorn SK, Grostern A, Lacrampe-Couloume G, Edwards EA, Mackinnon L, Repta C, Major DW, Sherwood Lollar B.J. Contam. Hydrol. 2007 Dec;94(3-4):249-60
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Sleep studies frequently lead to changes in respiratory support in children.Tan E, Nixon GM, Edwards EA.J Paediatr Child Health 2007 Jul-Aug;43(7-8):560-3
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Isotope analysis as a natural reaction probe to determine mechanisms of biodegradation of 1,2-dichloroethane.Hirschorn SK, Dinglasan-Panlilio MJ, Edwards EA, Lacrampe-Couloume G, Sherwood Lollar B.Environ. Microbiol. 2007 Jul;9(7):1651-7
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Growth and yields of dechlorinators, acetogens, and methanogens during reductive dechlorination of chlorinated ethenes and dihaloelimination of 1 ,2-dichloroethane.Duhamel M, Edwards EA.Environ. Sci. Technol. 2007 Apr;41(7):2303-10
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Identification and expression of benzylsuccinate synthase genes in a toluene-degrading methanogenic consortium.Washer CE, Edwards EA.Appl. Environ. Microbiol. 2007 Feb;73(4):1367-9
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2006

Top
Effects of trace element concentration on enzyme controlled stable isotope fractionation during aerobic biodegradation of toluene.Mancini SA, Hirschorn SK, Elsner M, Lacrampe-Couloume G, Sleep BE, Edwards EA, Lollar BS.Environ. Sci. Technol. 2006 Dec;40(24):7675-81
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Microbial composition of chlorinated ethene-degrading cultures dominated by Dehalococcoides.Duhamel M, Edwards EA.FEMS Microbiol. Ecol. 2006 Dec;58(3):538-49
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A 1,1,1-trichloroethane-degrading anaerobic mixed microbial culture enhances biotransformation of mixtures of chlorinated ethenes and ethanes.Grostern A, Edwards EA.Appl. Environ. Microbiol. 2006 Dec;72(12):7849-56
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Heparin derivatives as inhibitors of BACE-1, the Alzheimer's beta-secretase, with reduced activity against factor Xa and other proteases.Patey SJ, Edwards EA, Yates EA, Turnbull JE.J. Med. Chem. 2006 Oct;49(20):6129-32
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Evaluation of isotopic enrichment factors for the biodegradation of chlorinated ethenes using a parameter estimation model: toward an improved quantification of biodegradation.Morrill PL, Sleep BE, Slater GF, Edwards EA, Lollar BS.Environ. Sci. Technol. 2006 Jun;40(12):3886-92
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Biological enhancement of tetrachloroethene dissolution and associated microbial community changes.Sleep BE, Seepersad DJ, Kaiguo MO, Heidorn CM, Hrapovic L, Morrill PL, McMaster ML, Hood ED, Lebron C, Lollar BS, Major DW, Edwards EA.Environ. Sci. Technol. 2006 Jun;40(11):3623-33
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Harnessing microbial activities for environmental cleanup.Löffler FE, Edwards EA.Curr. Opin. Biotechnol. 2006 Jun;17(3):274-84
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Growth of Dehalobacter and Dehalococcoides spp. during degradation of chlorinated ethanes.Grostern A, Edwards EA.Appl. Environ. Microbiol. 2006 Jan;72(1):428-36
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2005

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Multiple reductive-dehalogenase-homologous genes are simultaneously transcribed during dechlorination by Dehalococcoides-containing cultures.Waller AS, Krajmalnik-Brown R, Löffler FE, Edwards EA.Appl. Environ. Microbiol. 2005 Dec;71(12):8257-64
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Metabolites detected during biodegradation of 13C6-benzene in nitrate-reducing and methanogenic enrichment cultures.Ulrich AC, Beller HR, Edwards EA.Environ. Sci. Technol. 2005 Sep;39(17):6681-91
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Carbon isotopic fractionation during aerobic vinyl chloride degradation.Chartrand MM, Waller A, Mattes TE, Elsner M, Lacrampe-Couloume G, Gossett JM, Edwards EA, Lollar BS.Environ. Sci. Technol. 2005 Feb;39(4):1064-70
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Quantifying chlorinated ethene degradation during reductive dechlorination at Kelly AFB using stable carbon isotopes.Morrill PL, Lacrampe-Couloume G, Slater GF, Sleep BE, Edwards EA, McMaster ML, Major DW, Sherwood Lollar B.J. Contam. Hydrol. 2005 Feb;76(3-4):279-93
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2004

Top
Gene expression profiles for detecting and distinguishing potential endocrine-disrupting compounds in environmental samples.Wang DY, McKague B, Liss SN, Edwards EA.Environ. Sci. Technol. 2004 Dec;38(23):6396-406
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Pathway dependent isotopic fractionation during aerobic biodegradation of 1,2-dichloroethane.Hirschorn SK, Dinglasan MJ, Elsner M, Mancini SA, Lacrampe-Couloume G, Edwards EA, Lollar BS.Environ. Sci. Technol. 2004 Sep;38(18):4775-81
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Characterization of a highly enriched dehalococcoides-containing culture that grows on vinyl chloride and trichloroethene.Duhamel M, Mo K, Edwards EA.Appl. Environ. Microbiol. 2004 Sep;70(9):5538-45
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Treatment of paediatric non-cystic fibrosis bronchiectasis.Edwards EA, Twiss J, Byrnes CA.Expert Opin Pharmacother 2004 Jul;5(7):1471-84
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Fluorotelomer alcohol biodegradation yields poly- and perfluorinated acids.Dinglasan MJ, Ye Y, Edwards EA, Mabury SA.Environ. Sci. Technol. 2004 May;38(10):2857-64
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Perchlorate-reducing microorganisms isolated from contaminated sites.Waller AS, Cox EE, Edwards EA.Environ. Microbiol. 2004 May;6(5):517-27
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Identification of estrogen-responsive genes by complementary deoxyribonucleic acid microarray and characterization of a novel early estrogen-induced gene: EEIG1.Wang DY, Fulthorpe R, Liss SN, Edwards EA.Mol. Endocrinol. 2004 Feb;18(2):402-11
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2003

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DNA microarrays for detecting endocrine-disrupting compounds.Francois E, Wang DY, Fulthorpe R, Liss SN, Edwards EA.Biotechnol. Adv. 2003 Dec;22(1-2):17-26
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Monitoring gene expression in mixed microbial communities by using DNA microarrays.Dennis P, Edwards EA, Liss SN, Fulthorpe R.Appl. Environ. Microbiol. 2003 Feb;69(2):769-78
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Physiological and molecular characterization of anaerobic benzene-degrading mixed cultures.Ulrich AC, Edwards EA.Environ. Microbiol. 2003 Feb;5(2):92-102
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Carbon and hydrogen isotopic fractionation during anaerobic biodegradation of benzene.Mancini SA, Ulrich AC, Lacrampe-Couloume G, Sleep B, Edwards EA, Lollar BS.Appl. Environ. Microbiol. 2003 Jan;69(1):191-8
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2002

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Field demonstration of successful bioaugmentation to achieve dechlorination of tetrachloroethene to ethene.Major DW, McMaster ML, Cox EE, Edwards EA, Dworatzek SM, Hendrickson ER, Starr MG, Payne JA, Buonamici LW.Environ. Sci. Technol. 2002 Dec;36(23):5106-16
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Comparison of anaerobic dechlorinating enrichment cultures maintained on tetrachloroethene, trichloroethene, cis-dichloroethene and vinyl chloride.Duhamel M, Wehr SD, Yu L, Rizvi H, Seepersad D, Dworatzek S, Cox EE, Edwards EA.Water Res. 2002 Oct;36(17):4193-202
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