Joshua Milstein

faculty_img Academic Title: Associate Professor

Campus: UTM

CSB Appointment: Cross Appointment

Primary Undergraduate Department:
Physics, UTM

Graduate Programs:
Cell & Systems Biology

Titles and Honors:

Academic or Administrative Appointments:

Education:
Ph.D. University of Colorado 2004

 

Mailing Address
Department of Chemical and Physical Sciences
University of Toronto
3359 Mississauga Road North
Mississauga, ON L5L 1C6
Canada

 

Contact Information
Office phone: 905-569-4598
Office: DV 4053
Lab:  DV 5032
Lab phone: 905-828-5436
Email: josh.milstein@utoronto.ca
URL: http://www.utm.utoronto.ca/milsteinlab/

 

Research Areas
Bioinformatics
Biotechnology
Molecular Biology
Quantitative Biology

 

Research

My research focuses on studying the conformational dynamics of DNA, which, while being intimately involved in genetic function, has operational effects that are not well understood at present. Errors in genetic expression, besides arising from mistakes in the code itself, could also manifest by macromolecules that over-twist or over-stretch the DNA, problems in packing within the nucleus, long-range interference between genes, and so on. Studying the dynamics of DNA, while challenging, has the potential to define a new paradigm for our understanding of cellular function, which could have a range of implications for our understanding of health and disease.

 

Publications

1: Singh K, Milstein JN, Navarre WW. Xenogeneic Silencing and Its Impact on
Bacterial Genomes. Annu Rev Microbiol. 2016 Jun 17. [Epub ahead of print] PubMed
PMID: 27359215.

2: Mazouchi A, Milstein JN. Fast Optimized Cluster Algorithm for Localizations
(FOCAL): a spatial cluster analysis for super-resolved microscopy.
Bioinformatics. 2016 Mar 1;32(5):747-54. doi: 10.1093/bioinformatics/btv630. Epub
2015 Nov 4. PubMed PMID: 26543172.

3: Pollari R, Milstein JN. Improved axial trapping with holographic optical
tweezers. Opt Express. 2015 Nov 2;23(22):28857-67. doi: 10.1364/OE.23.028857.
PubMed PMID: 26561154.

4: Wang H, Milstein JN. Simulation Assisted Analysis of the Intrinsic Stiffness
for Short DNA Molecules Imaged with Scanning Atomic Force Microscopy. PLoS One.
2015 Nov 4;10(11):e0142277. doi: 10.1371/journal.pone.0142277. eCollection 2015.
PubMed PMID: 26535902; PubMed Central PMCID: PMC4633100.

5: Yehoshua S, Pollari R, Milstein JN. Axial Optical Traps: A New Direction for
Optical Tweezers. Biophys J. 2015 Jun 16;108(12):2759-66. doi:
10.1016/j.bpj.2015.05.014. Review. PubMed PMID: 26083913; PubMed Central PMCID:
PMC4472083.

6: Wang H, Yehoshua S, Ali SS, Navarre WW, Milstein JN. A biomechanical mechanism
for initiating DNA packaging. Nucleic Acids Res. 2014 Oct 29;42(19):11921-7. doi:
10.1093/nar/gku896. Epub 2014 Oct 1. PubMed PMID: 25274732; PubMed Central PMCID:
PMC4231757.

For an up to date list of publications please visit: http://www.utm.utoronto.ca/milsteinlab/publications/.