Keiko Yoshioka
Professor
Campus
St. George (downtown)
CSB Appointment
Full
Research Areas
Genetics / Genomics, Molecular Biology, Pathology, Plant Biology, Structural Biology, Systems Biology
Education
Ph.D. Hokkaido University 1994
M.Sc. Hokkaido University 1991
B.A. Hokkaido University 1989
Primary Undergraduate Department
Cell & Systems Biology
Graduate Programs
Cell & Systems Biology
Research Description
Understanding the molecular mechanism of plant defense systems is critical in determining how plants defend themselves against various stresses, including pathogens and applying this knowledge to improve agricultural production and protect environment against possible negative impact from pesticides and fungicides. The long-term goal of my laboratory is to understand how plants transmit the intra- and inter-cellular signals of various environmental stresses including pathogen infection. To achieve this goal, we are using both forward and reverse genetics approaches to identifying novel components (genes/proteins) that are involved in plant immunity. One group of genes that we have identified are cyclic nucleotide-gated ion channels (CNGCs). They form a large gene family and affect multiple aspects of plant physiology such as pollen development, thermal sensing and immunity, likely through Ca2+ signaling. Using molecular, genetic and biochemical analyses, we are investigating the molecular basis of CNGCs. Other aspects we focus on are environmental effects on plant immunity. It has been known that environmental condition such as temperature and humidity significantly affect the activation of defense responses. This suggests that plants possess an elaborate signaling network to fine-tune the responses against multiple stresses. We investigate the signal crosstalk between abiotic and biotic stress signal transduction to understand the complexity of the signaling network and apply this knowledge for developing novel technology to protect plants and environment.
Contact Information
Office Phone: 416-978-3545
Office: ESC3070
Lab: ESC3067
Lab Phone: 416-946-0444
Email
Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Willcocks St.
Toronto, ON M5S 3B2
Canada
Recent News
Publications
2022
Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots
Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack MK, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J, Costa A, Vanneste S, Napier R
2022, Journal of Experimental Botany, 10.1093/jxb/erac019
Arabidopsis thaliana CYCLIC NUCLEOTIDE‐GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis
Wang L, Ning Y, Sun J, Wilkins KA, Matthus E, McNelly RE, Dark A, Rubio L, Moeder W, Yoshioka K, Véry A, Stacey G, Leblanc‐Fournier N, Legué V, Moulia B, Davies JM
2022, New Phytologist, 10.1111/nph.17987
A tale of many families: calcium channels in plant immunity
Xu G, Moeder W, Yoshioka K, Shan L
2022, The Plant Cell, 10.1093/plcell/koac033
2021
A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants
Wang Y, Gong Q, Wu Y, Huang F, Ismayil A, Zhang D, Li H, Gu H, Ludman M, Fátyol K, Qi Y, Yoshioka K, Hanley-Bowdoin L, Hong Y, Liu Y
2021, Cell Host & Microbe, 10.1016/j.chom.2021.07.003
CYCLIC NUCLEOTIDE-GATED ION CHANNEL 2 modulates auxin homeostasis and signaling
Chakraborty S, Toyota M, Moeder W, Chin K, Fortuna A, Champigny M, Vanneste S, Gilroy S, Beeckman T, Nambara E, Yoshioka K
2021, Plant Physiology, 10.1093/plphys/kiab332
Multiple phosphorylation events of the mitochondrial membrane protein, TTM1, regulate cell death during senescence
Karia P, Yoshioka K, Moeder W
2021, The Plant Journal, 10.1111/tpj.15470
2020
Plant Cyclic Nucleotide-Gated Channels: New Insights on Their Functions and Regulation
Dietrich P, Moeder W, Yoshioka K
2020, Plant Physiology, 10.1104/pp.20.00425
Calcium channel in plants helps shut the door on intruders
Yoshioka K, Moeder W
2020, Nature, 10.1038/d41586-020-02504-0
2019
A host–pathogen interactome uncovers phytopathogenic strategies to manipulate plant ABA responses
Cao FY, Khan M, Taniguchi M, Mirmiran A, Moeder W, Lumba S, Yoshioka K, Desveaux D
2019, The Plant Journal, 10.1111/tpj.14425
The Receptor Kinases BAK1/SERK4 Regulate Ca2+ Channel-Mediated Cellular Homeostasis for Cell Death Containment
Yu X, Xu G, Li B, de Souza Vespoli L, Liu H, Moeder W, Chen S, de Oliveira MV, Ariádina de Souza S, Shao W, Rodrigues B, Ma Y, Chhajed S, Xue S, Berkowitz GA, Yoshioka K, He P, Shan L
2019, Current Biology, 10.1016/j.cub.2019.09.018
Ca2+ to the rescue – Ca2+channels and signaling in plant immunity
Moeder W, Phan V, Yoshioka K
2019, Plant Science, 10.1016/j.plantsci.2018.04.012
2018
Arabidopsis ETHYLENE RESPONSE FACTOR 8 (ERF8) has dual functions in ABA signaling and immunity
Cao FY, DeFalco TA, Moeder W, Li B, Gong Y, Liu X, Taniguchi M, Lumba S, Toh S, Shan L, Ellis B, Desveaux D, Yoshioka K
2018, BMC Plant Biology, 10.1186/s12870-018-1402-6
A novel role for Cyclic Nucleotide-Gated Ion Channel 2 (DND1) in auxin signaling
Chakraborty S, Toyota M, Moeder W, Chin K, Fortuna A, Champigny M, Vanneste S, Gilroy S, Beeckman T, Yoshioka K
2018, , 10.1101/508572
2017
CNGCs break through-A rice cyclic nucleotide-gated channel paves the way for pollen tube growth.
Moeder W, Yoshioka K
2017, PLoS genetics, 13, e1007066, 29190294
Translatome analysis of an NB-LRR immune response identifies important contributors to plant immunity in Arabidopsis
Meteignier L, El Oirdi M, Cohen M, Barff T, Matteau D, Lucier J, Rodrigue S, Jacques P, Yoshioka K, Moffett P
2017, Journal of Experimental Botany, 10.1093/jxb/erx078
Calmodulin as a Ca2+-Sensing Subunit of Arabidopsis Cyclic Nucleotide-Gated Channel Complexes
Fischer C, DeFalco TA, Karia P, Snedden WA, Moeder W, Yoshioka K, Dietrich P
2017, Plant and Cell Physiology, 10.1093/pcp/pcx052
Using GCaMP3 to Study Ca2+ Signaling in Nicotiana Species
DeFalco TA, Toyota M, Phan V, Karia P, Moeder W, Gilroy S, Yoshioka K
2017, Plant and Cell Physiology, doi:10.1093/pcp/pcx053
Triphosphate Tunnel Metalloenzyme Function in Senescence Highlights a Biological Diversification of This Protein Superfamily
Ung H, Karia P, Ebine K, Ueda T, Yoshioka K, Moeder W
2017, Plant Physiology, 10.1104/pp.17.00700
Microbial diversity in leaves, trunk and rhizosphere of coconut palms (Cocos nucifera L.) associated with the coconut lethal yellowing phytoplasma in Grand-Lahou, Cte dIvoire
Nadia PM, Ahmed H, Henry ST, Tacra TL, Julia C, Pauline W, Hortense AD, Jean LKK, Keiko Y, Wolfgang M, James S, Yaima AR
2017, African Journal of Biotechnology, 10.5897/AJB2017.16006
2016
Opening the Gates: Insights into Cyclic Nucleotide-Gated Channel-Mediated Signaling.
DeFalco TA, Moeder W, Yoshioka K
2016, Trends in plant science, 21, 903-906, 27623305
Multiple Calmodulin-binding Sites Positively and Negatively Regulate Arabidopsis CYCLIC NUCLEOTIDE-GATED CHANNEL12
DeFalco TA, Marshall CB, Munro K, Kang H, Moeder W, Ikura M, Snedden WA, Yoshioka K
2016, The Plant Cell, 10.1105/tpc.15.00870
2014
Arabidopsis triphosphate tunnel metalloenzyme2 is a negative regulator of the salicylic Acid-mediated feedback amplification loop for defense responses
Ung H, Moeder W, Yoshioka K
2014, Plant physiology, 166, 1009-21, 25185123
2013
Crystal structure and biochemical analyses reveal that the Arabidopsis triphosphate tunnel metalloenzyme AtTTM3 is a tripolyphosphatase involved in root development
Moeder W, Garcia-Petit C, Ung H, Fucile G, Samuel MA, Christendat D, Yoshioka K
2013, The Plant journal : for cell and molecular biology, 76, 615-26, 24004165
Expression and gene knockdown of zebrafish Ca(2+)/calmodulin-dependent protein kinase Iδ-LL
Senga Y, Yoshioka K, Kameshita I, Sueyoshi N
2013, Archives of biochemistry and biophysics, 540, 41-52, 24099663
The Arabidopsis cyclic nucleotide-gated ion channels AtCNGC2 and AtCNGC4 work in the same signaling pathway to regulate pathogen defense and floral transition
Chin K, DeFalco TA, Moeder W, Yoshioka K
2013, Plant physiology, 163, 611-24, 24027242
A suppressor screen of the chimeric AtCNGC11/12 reveals residues important for intersubunit interactions of cyclic nucleotide-gated ion channels
Abdel-Hamid H, Chin K, Moeder W, Shahinas D, Gupta D, Yoshioka K
2013, Plant physiology, 162, 1681-93, 23735507
2012
Texture, sensory and swallowing characteristics of high-pressure-heat-treated pork meat gel as a dysphagia diet
Tokifuji A, Matsushima Y, Hachisuka K, Yoshioka K
2013, Meat science, 93, 843-8, 23313970
Potential inaccuracies in chloride measurements in patients with severe metabolic acidosis
Makiishi T, Nishimura N, Yoshioka K, Yamamoto S, Mitsuhashi R, Maeda S, Konishi T, Hirose K
2012, International journal of nephrology, 2012, 768316, 22778953
2011
High throughput chemical screening supports the involvement of Ca2+ in cyclic nucleotide-gated ion channel-mediated programmed cell death in Arabidopsis
Abdel-Hamid H, Chin K, Moeder W, Yoshioka K
2011, Plant signaling & behavior, 6, 1817-9, 22041991
Altered germination and subcellular localization patterns for PUB44/SAUL1 in response to stress and phytohormone treatments
Salt JN, Yoshioka K, Moeder W, Goring DR
2011, PloS one, 6, e21321, 21738636
The role of cyclic nucleotide-gated ion channels in plant immunity
Moeder W, Urquhart W, Ung H, Yoshioka K
2011, Molecular plant, 4, 442-52, 21459831
The cyclic nucleotide-gated channels AtCNGC11 and 12 are involved in multiple Ca²⁺-dependent physiological responses and act in a synergistic manner
Urquhart W, Chin K, Ung H, Moeder W, Yoshioka K
2011, Journal of experimental botany, 62, 3671-82, 21414958
The roles of ABA in plant-pathogen interactions
Cao FY, Yoshioka K, Desveaux D
2011, Journal of plant research, 124, 489-99, 21380629
2010
SA-ABA antagonism in defense responses
Moeder W, Ung H, Mosher S, Yoshioka K
2010, Plant signaling & behavior, 5, 1231-3, 20861686
Calmodulin binding to Arabidopsis cyclic nucleotide gated ion channels
Abdel-Hamid H, Chin K, Shahinas D, Moeder W, Yoshioka K
2010, Plant signaling & behavior, 5, 1147-9, 21150265
Leaf senescence signaling: the Ca2+-conducting Arabidopsis cyclic nucleotide gated channel2 acts through nitric oxide to repress senescence programming
Ma W, Smigel A, Walker RK, Moeder W, Yoshioka K, Berkowitz GA
2010, Plant physiology, 154, 733-43, 20699402
Importance of the alphaC-helix in the cyclic nucleotide binding domain for the stable channel regulation and function of cyclic nucleotide gated ion channels in Arabidopsis
Chin K, Moeder W, Abdel-Hamid H, Shahinas D, Gupta D, Yoshioka K
2010, Journal of experimental botany, 61, 2383-93, 20378667
The lesion-mimic mutant cpr22 shows alterations in abscisic acid signaling and abscisic acid insensitivity in a salicylic acid-dependent manner
Mosher S, Moeder W, Nishimura N, Jikumaru Y, Joo SH, Urquhart W, Klessig DF, Kim SK, Nambara E, Yoshioka K
2010, Plant physiology, 152, 1901-13, 20164209
2009
Forward and reverse genetics to identify genes involved in the age-related resistance response in Arabidopsis thaliana
Carviel JL, Al-Daoud F, Neumann M, Mohammad A, Provart NJ, Moeder W, Yoshioka K, Cameron RK
2009, Molecular plant pathology, 10, 621-34, 19694953
2008
Lesion mimic mutants: A classical, yet still fundamental approach to study programmed cell death
Moeder W, Yoshioka K
2008, Plant signaling & behavior, 3, 764-7, 19513227
Identification of a functionally essential amino acid for Arabidopsis cyclic nucleotide gated ion channels using the chimeric AtCNGC11/12 gene
Baxter J, Moeder W, Urquhart W, Shahinas D, Chin K, Christendat D, Kang HG, Angelova M, Kato N, Yoshioka K
2008, The Plant journal : for cell and molecular biology, 56, 457-69, 18643993
2007
Cyclic nucleotide gated channels and ca-mediated signal transduction during plant innate immune response to pathogens
Ma W, Yoshioka K, Berkowitz GA
2007, Plant signaling & behavior, 2, 548-50, 19704555
The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca2+ dependent manner
Urquhart W, Gunawardena AH, Moeder W, Ali R, Berkowitz GA, Yoshioka K
2007, Plant molecular biology, 65, 747-61, 17885810
2006
NPS6, encoding a nonribosomal peptide synthetase involved in siderophore-mediated iron metabolism, is a conserved virulence determinant of plant pathogenic ascomycetes
Oide S, Moeder W, Krasnoff S, Gibson D, Haas H, Yoshioka K, Turgeon BG
2006, The Plant cell, 18, 2836-53, 17056706
The chimeric Arabidopsis CYCLIC NUCLEOTIDE-GATED ION CHANNEL11/12 activates multiple pathogen resistance responses
Yoshioka K, Moeder W, Kang HG, Kachroo P, Masmoudi K, Berkowitz G, Klessig DF
2006, The Plant cell, 18, 747-63, 16461580
2005
Involvement of the small GTPase Rac in the defense responses of tobacco to pathogens
Moeder W, Yoshioka K, Klessig DF
2005, Molecular plant-microbe interactions : MPMI, 18, 116-24, 15720080