Keiko Yoshioka

faculty_img Academic Title: Associate Professor

Campus: St. George

CSB Appointment: Full

Primary Undergraduate Department:
Cell & Systems Biology

Graduate Programs:
Cell & Systems Biology

Titles and Honors:
Academic or Administrative Appointments:
Education:
Ph.D. Hokkaido University 1994 M.Sc. Hokkaido University 1991 B.A. Hokkaido University 1989

 

Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Willcocks St.
Toronto, ON M5S 3B2
Canada

 

Contact Information
Office phone: 416-978-3545
Office: ESC 3070
Lab: ESC 3067
Lab phone: 416-946-0444
Email: keiko.yoshioka@utoronto.ca
URL: http://labs.csb.utoronto.ca/yoshioka

 

Research Areas
Biotechnology
Cell Biology
Functional Genomics
Genetics
Molecular Biology
Plant Biology
Structural Biology
Systems Biology

 

Research

Understanding the molecular mechanism of plant defense systems is critical in determining how plants defend themselves against various stresses, including pathogens and applying this knowledge to improve agricultural production and protect environment against possible negative impact from pesticides and fungicides. The long-term goal of my laboratory is to understand how plants transmit the intra- and inter-cellular signals of various environmental stresses including pathogen infection. To achieve this goal, we are using both forward and reverse genetics approaches to identifying novel components (genes/proteins) that are involved in plant immunity. One group of genes that we have identified are cyclic nucleotide-gated ion channels (CNGCs). They form a large gene family and affect multiple aspects of plant physiology such as pollen development, thermal sensing and immunity, likely through Ca2+ signaling. Using molecular, genetic and biochemical analyses, we are investigating the molecular basis of CNGCs. Other aspects we focus on are environmental effects on plant immunity. It has been known that environmental condition such as temperature and humidity significantly affect the activation of defense responses. This suggests that plants possess an elaborate signaling network to fine-tune the responses against multiple stresses. We investigate the signal crosstalk between abiotic and biotic stress signal transduction to understand the complexity of the signaling network and apply this knowledge for developing novel technology to protect plants and environment.

 

Publications

 


2016
2014
2013
2012
2011
2010
2009
2008
2007
2006
2005
2004
 

2016

Top

Multiple Calmodulin-Binding Sites Positively and Negatively Regulate Arabidopsis CYCLIC NUCLEOTIDE-GATED CHANNEL12. DeFalco, Thomas A, Christopher B Marshall, Kim Munro, Hong-Gu Kang, Wolfgang Moeder, Mitsuhiko Ikura, Wayne A Snedden, and Keiko Yoshioka. The Plant cell, no. 7 ( 22). 2016 doi:10.1105/tpc.15.00870
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Opening the Gates: Insights into Cyclic Nucleotide-Gated Channel-Mediated Signaling Wolfgang Moeder, and Keiko Yoshioka. Trends in plant science ( 9). 1016 doi:S1360-1385(16)30122-4.
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2014

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Arabidopsis triphosphate tunnel metalloenzyme2 is a negative regulator of the salicylic Acid-mediated feedback amplification loop for defense responses.Ung H, Moeder W, Yoshioka K.Plant Physiol. 2014 Oct;166(2):1009-21
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2013

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The Arabidopsis cyclic nucleotide-gated ion channels AtCNGC2 and AtCNGC4 work in the same signaling pathway to regulate pathogen defense and floral transition.Chin K, DeFalco TA, Moeder W, Yoshioka K.Plant Physiol. 2013 Oct;163(2):611-24
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Crystal structure and biochemical analyses reveal that the Arabidopsis triphosphate tunnel metalloenzyme AtTTM3 is a tripolyphosphatase involved in root development.Moeder W, Garcia-Petit C, Ung H, Fucile G, Samuel MA, Christendat D, Yoshioka K.Plant J. 2013 Nov;76(4):615-26
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A suppressor screen of the chimeric AtCNGC11/12 reveals residues important for intersubunit interactions of cyclic nucleotide-gated ion channels.Abdel-Hamid H, Chin K, Moeder W, Shahinas D, Gupta D, Yoshioka K.Plant Physiol. 2013 Jul;162(3):1681-93
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2012

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2011

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High throughput chemical screening supports the involvement of Ca2+ in cyclic nucleotide-gated ion channel-mediated programmed cell death in Arabidopsis.Abdel-Hamid H, Chin K, Moeder W, Yoshioka K.Plant Signal Behav 2011 Nov;6(11):1817-9
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Altered germination and subcellular localization patterns for PUB44/SAUL1 in response to stress and phytohormone treatments.Salt JN, Yoshioka K, Moeder W, Goring DR.PLoS ONE 2011;6(6):e21321
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The role of cyclic nucleotide-gated ion channels in plant immunity.Moeder W, Urquhart W, Ung H, Yoshioka K.Mol Plant 2011 May;4(3):442-52
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The cyclic nucleotide-gated channels AtCNGC11 and 12 are involved in multiple Ca²⁺-dependent physiological responses and act in a synergistic manner.Urquhart W, Chin K, Ung H, Moeder W, Yoshioka K.J. Exp. Bot. 2011 Jun;62(10):3671-82
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The roles of ABA in plant-pathogen interactions.Cao FY, Yoshioka K, Desveaux D.J. Plant Res. 2011 Jul;124(4):489-99
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2010

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Calmodulin binding to Arabidopsis cyclic nucleotide gated ion channels.Abdel-Hamid H, Chin K, Shahinas D, Moeder W, Yoshioka K.Plant Signal Behav 2010 Sep;5(9):1147-9
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SA-ABA antagonism in defense responses.Moeder W, Ung H, Mosher S, Yoshioka K.Plant Signal Behav 2010 Oct;5(10):1231-3
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Leaf senescence signaling: the Ca2+-conducting Arabidopsis cyclic nucleotide gated channel2 acts through nitric oxide to repress senescence programming.Ma W, Smigel A, Walker RK, Moeder W, Yoshioka K, Berkowitz GA.Plant Physiol. 2010 Oct;154(2):733-43
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Importance of the alphaC-helix in the cyclic nucleotide binding domain for the stable channel regulation and function of cyclic nucleotide gated ion channels in Arabidopsis.Chin K, Moeder W, Abdel-Hamid H, Shahinas D, Gupta D, Yoshioka K.J. Exp. Bot. 2010 May;61(9):2383-93
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The lesion-mimic mutant cpr22 shows alterations in abscisic acid signaling and abscisic acid insensitivity in a salicylic acid-dependent manner.Mosher S, Moeder W, Nishimura N, Jikumaru Y, Joo SH, Urquhart W, Klessig DF, Kim SK, Nambara E, Yoshioka K.Plant Physiol. 2010 Apr;152(4):1901-13
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2009

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Forward and reverse genetics to identify genes involved in the age-related resistance response in Arabidopsis thaliana.Carviel JL, Al-Daoud F, Neumann M, Mohammad A, Provart NJ, Moeder W, Yoshioka K, Cameron RK.Mol. Plant Pathol. 2009 Sep;10(5):621-34
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2008

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Lesion mimic mutants: A classical, yet still fundamental approach to study programmed cell death.Moeder W, Yoshioka K.Plant Signal Behav 2008 Oct;3(10):764-7
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Identification of a functionally essential amino acid for Arabidopsis cyclic nucleotide gated ion channels using the chimeric AtCNGC11/12 gene.Baxter J, Moeder W, Urquhart W, Shahinas D, Chin K, Christendat D, Kang HG, Angelova M, Kato N, Yoshioka K.Plant J. 2008 Nov;56(3):457-69
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2007

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Cyclic nucleotide gated channels and ca-mediated signal transduction during plant innate immune response to pathogens.Ma W, Yoshioka K, Berkowitz GA.Plant Signal Behav 2007 Nov;2(6):548-50
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The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca2+ dependent manner.Urquhart W, Gunawardena AH, Moeder W, Ali R, Berkowitz GA, Yoshioka K.Plant Mol. Biol. 2007 Dec;65(6):747-61
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2006

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NPS6, encoding a nonribosomal peptide synthetase involved in siderophore-mediated iron metabolism, is a conserved virulence determinant of plant pathogenic ascomycetes.Oide S, Moeder W, Krasnoff S, Gibson D, Haas H, Yoshioka K, Turgeon BG.Plant Cell 2006 Oct;18(10):2836-53
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The chimeric Arabidopsis CYCLIC NUCLEOTIDE-GATED ION CHANNEL11/12 activates multiple pathogen resistance responses.Yoshioka K, Moeder W, Kang HG, Kachroo P, Masmoudi K, Berkowitz G, Klessig DF.Plant Cell 2006 Mar;18(3):747-63
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2005

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Involvement of the small GTPase Rac in the defense responses of tobacco to pathogens.Moeder W, Yoshioka K, Klessig DF.Mol. Plant Microbe Interact. 2005 Feb;18(2):116-24
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2004

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High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression.Zhou F, Menke FL, Yoshioka K, Moder W, Shirano Y, Klessig DF.Plant J. 2004 Sep;39(6):920-32
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