Penney Gilbert
Associate Professor & Canada Research Chair, Endogenous Repair
Campus
St. George (downtown)
CSB Appointment
Cross
Research Areas
Cell Biology
Education
Ph.D., University of Pennsylvania 2006
BSc, Haverford College 1999
Primary Undergraduate Department
Institute of Biomedical Engineering
Graduate Programs
Cell and Systems Biology
Institute of Biomedical Engineering
Academic or Administrative Appointments
Advisor, Ontario Institute for Regenerative Medicine
Research Description
The native stem cell niche is a three-dimensional entity. While conceptually it is accepted that dimensionality is a critical feature of tissues that defines the location and timing of cellular events, understanding how dimensionality exerts such a powerful influence on stem cell biology is not well understood. Our research tackles this quandary by defining the interactions between muscle stem cells and their dynamic extracellular milieu that serve to orchestrate the elegant process by which a muscle stem cell switches between states of quiescence, activation, proliferation, differentiation, and self-renewal in health and in disease. Our specific emphasis is on evaluating how biomechanical stresses of the niche, like compressive forces, shear stress, or extracellular matrix stiffness, synergize with niche proteins to drive stem cell behavior. By quantifying in vivo biomechanical stresses presiding over the quiescent and regenerating adult skeletal muscle niche, and engineering three-dimensional culture models of skeletal muscle regeneration to deconstruct and reconstruct the 3D niche, we uncover new insights into how the native three-dimensional tissue exerts spatiotemporal control over muscle stem cell fate.
Contact Information
Office Phone: 416-978-2501
Office: CCBR 1116
Lab: CCBR 1140
Lab Phone: 416-978-2507
Email
Mailing Address
164 College Street,
Rosebrugh Building Rm. 407,
Toronto, Ontario,
M5S3G9
Publications
2021
MEndR: An In Vitro Functional Assay to Predict In Vivo Muscle Stem Cell‐Mediated Repair
Davoudi S, Xu B, Jacques E, Cadavid JL, McFee M, Chin C, Meysami A, Ebrahimi M, Bakooshli MA, Tung K, Ahn H, Ginsberg HJ, McGuigan AP, Gilbert PM
2021, Advanced Functional Materials, 10.1002/adfm.202106548
2020
A 96-well culture platform enables longitudinal analyses of engineered human skeletal muscle microtissue strength
Afshar ME, Abraha HY, Bakooshli MA, Davoudi S, Thavandiran N, Tung K, Ahn H, Ginsberg HJ, Zandstra PW, Gilbert PM
2020, Scientific Reports, 10.1038/s41598-020-62837-8
Functional arrays of human pluripotent stem cell-derived cardiac microtissues
Thavandiran N, Hale C, Blit P, Sandberg ML, McElvain ME, Gagliardi M, Sun B, Witty A, Graham G, Do V, Bakooshli MA, Le H, Ostblom J, McEwen S, Chau E, Prowse A, Fernandes I, Norman A, Gilbert PM, Keller G, Tagari P, Xu H, Radisic M, Zandstra PW
2020, Scientific Reports, 10.1038/s41598-020-62955-3
Engineering the next generation of human skeletal muscle models: From cellular complexity to disease modeling
Moyle LA, Jacques E, Gilbert PM
2020, Current Opinion in Biomedical Engineering, 10.1016/j.cobme.2020.05.006
Three-dimensional niche stiffness synergizes with Wnt7a to modulate the extent of satellite cell symmetric self-renewal divisions
Moyle LA, Cheng RY, Liu H, Davoudi S, Ferreira SA, Nissar AA, Sun Y, Gentleman E, Simmons CA, Gilbert PM, Discher D
2020, Molecular Biology of the Cell, 10.1091/mbc.E20-01-0078
2019
A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction
Afshar Bakooshli M, Lippmann ES, Mulcahy B, Iyer N, Nguyen CT, Tung K, Stewart BA, van den Dorpel H, Fuehrmann T, Shoichet M, Bigot A, Pegoraro E, Ahn H, Ginsberg H, Zhen M, Ashton RS, Gilbert PM
2019, eLife, 10.7554/eLife.44530
A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations
Nixon AML, Duque A, Yelle N, McLaughlin M, Davoudi S, Pedley NM, Haynes J, Brown KR, Pan J, Hart T, Gilbert PM, Singh SK, O’Brien CA, Sidhu SS, Moffat J
2019, Scientific Reports, 10.1038/s41598-018-37462-1
Cored in the act: the use of models to understand core myopathies
Fusto A, Moyle LA, Gilbert PM, Pegoraro E
2019, Disease Models & Mechanisms, 10.1242/dmm.041368
2018
Muscle stem cell intramuscular delivery within hyaluronan methylcellulose improves engraftment efficiency and dispersion
Davoudi S, Chin C, Cooke MJ, Tam RY, Shoichet MS, Gilbert PM
2018, Biomaterials, 10.1016/j.biomaterials.2018.04.048
Cellular Biomechanics in Skeletal Muscle Regeneration
Li EW, McKee-Muir OC, Gilbert PM
2018, , 10.1016/bs.ctdb.2017.08.007
2017
Optimization of Satellite Cell Culture Through Biomaterials
Davoudi S, Gilbert PM
2017, , 10.1007/978-1-4939-6771-1_18
Stage-specific differences in secretory profile of mesenchymal stromal cells (MSCs) subjected to early- vs late-stage OA synovial fluid
Gómez-Aristizábal A, Sharma A, Bakooshli M, Kapoor M, Gilbert P, Viswanathan S, Gandhi R
2017, Osteoarthritis and Cartilage, 10.1016/j.joca.2016.11.010
Cellular adaptation to biomechanical stress across length scales in tissue homeostasis and disease
Gilbert PM, Weaver VM
2017, Seminars in Cell & Developmental Biology, 10.1016/j.semcdb.2016.09.004
Cellular adaptation to biomechanical stress across length scales in tissue homeostasis and disease
Gilbert PM, Weaver VM
2017, Seminars in Cell & Developmental Biology, 10.1016/j.semcdb.2016.09.004
2016
Biomechanical Origins of Muscle Stem Cell Signal Transduction
Morrissey JB, Cheng RY, Davoudi S, Gilbert PM
2016, Journal of Molecular Biology, 10.1016/j.jmb.2015.05.004
Targeting the stem cell niche with regenerative biomaterials
Nissar AA, Martowirogo A, Gilbert PM
2016, Current Opinion in Solid State and Materials Science, 10.1016/j.cossms.2016.03.003
2015
Global Linking of Cell Tracks Using the Viterbi Algorithm
Magnusson KEG, Jalden J, Gilbert PM, Blau HM
2015, IEEE Transactions on Medical Imaging, 10.1109/TMI.2014.2370951
2014
Tissue mechanics modulate microRNA-dependent PTEN expression to regulate malignant progression
Mouw JK, Yui Y, Damiano L, Bainer RO, Lakins JN, Acerbi I, Ou G, Wijekoon AC, Levental KR, Gilbert PM, Hwang ES, Chen Y, Weaver VM
2014, Nature Medicine, 10.1038/nm.3497
Rejuvenation of the muscle stem cell population restores strength to injured aged muscles
Cosgrove BD, Gilbert PM, Porpiglia E, Mourkioti F, Lee SP, Corbel SY, Llewellyn ME, Delp SL, Blau HM
2014, Nature Medicine, 10.1038/nm.3464
2012
Protein-Engineered Biomaterials to Generate Human Skeletal Muscle Mimics
Sengupta D, Gilbert PM, Johnson KJ, Blau HM, Heilshorn SC
2012, Advanced Healthcare Materials, 10.1002/adhm.201200195
A single cell bioengineering approach to elucidate mechanisms of adult stem cell self-renewal
Gilbert PM, Corbel S, Doyonnas R, Havenstrite K, Magnusson KEG, Blau HM
2012, Integrative Biology, 10.1039/c2ib00148a
2011
Single-cell phospho-specific flow cytometric analysis demonstrates biochemical and functional heterogeneity in human hematopoietic stem and progenitor compartments
Gibbs KD, Gilbert PM, Sachs K, Zhao F, Blau HM, Weissman IL, Nolan GP, Majeti R
2011, Blood, 10.1182/blood-2010-07-298232
Engineering a stem cell house into a home
Gilbert PM, Blau HM
2011, Stem Cell Research & Therapy, 10.1186/scrt44
2010
Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
Gilbert PM, Havenstrite KL, Magnusson KEG, Sacco A, Leonardi NA, Kraft P, Nguyen NK, Thrun S, Lutolf MP, Blau HM
2010, Science, 10.1126/science.1191035
HOXA9 regulates BRCA1 expression to modulate human breast tumor phenotype
Gilbert PM, Mouw JK, Unger MA, Lakins JN, Gbegnon MK, Clemmer VB, Benezra M, Licht JD, Boudreau NJ, Tsai KK, Welm AL, Feldman MD, Weber BL, Weaver VM
2010, Journal of Clinical Investigation, 10.1172/JCI39534
2009
Designing materials to direct stem-cell fate
Lutolf MP, Gilbert PM, Blau HM
2009, Nature, 10.1038/nature08602
A home away from home: Challenges and opportunities in engineering in vitro muscle satellite cell niches
Cosgrove BD, Sacco A, Gilbert PM, Blau HM
2009, Differentiation, 10.1016/j.diff.2009.08.004
2005
Saccharomyces cerevisiae Rab-GDI Displacement Factor Ortholog Yip3p Forms Distinct Complexes with the Ypt1 Rab GTPase and the Reticulon Rtn1p
Geng J, Shin ME, Gilbert PM, Collins RN, Burd CG
2005, Eukaryotic Cell, 10.1128/EC.4.7.1166-1174.2005
2004
Watch thy neighbor: cancer is a communal affair
Weaver VM
2004, Journal of Cell Science, 10.1242/jcs.01137
2003
Dual Prenylation Is Required for Rab Protein Localization and Function
Calero M, Chen CZ, Zhu W, Winand N, Havas KA, Gilbert PM, Burd CG, Collins RN
2003, Molecular Biology of the Cell, 10.1091/mbc.e02-11-0707
2001
FYVE Domain Targets Pib1p Ubiquitin Ligase to Endosome and Vacuolar Membranes
Shin ME, Ogburn KD, Varban OA, Gilbert PM, Burd CG
2001, Journal of Biological Chemistry, 10.1074/jbc.M105665200