Rene Harrison

faculty_img Academic Title: Associate Professor

Campus: UTSC

CSB Appointment: Full

Primary Undergraduate Department:
Biological Sciences, UTSC

Graduate Programs:
Cell & Systems Biology
Laboratory Medicine & Pathobiology

Titles and Honors:


Academic or Administrative Appointments:
Associate Chair for Research

Education:
Ph.D. University of Toronto 2000 M.Sc. University of Manitoba 1996

 

Mailing Address
Department of Cell & Systems Biology
University of Toronto
1265 Military Trail
Scarborough, ON M1C 1A4
Canada
 

 

Contact Information
Office phone: 416-287-7377 
Office: SW 415A 
Lab: SW 422 
Lab phone: 416-287-7440 
Email: harrison@utsc.utoronto.ca 
URL:

 

Research Areas
Cell Biology

 

Research

As a cell biologist, our lab studies the inner workings of immune cells called macrophages. Macrophages are crucial frontline cells in the body’s defense against infection. We also study the bone cells: osteoclasts and osteoblasts. We make regular use of cell culture, biochemistry and molecular biology to manipulate these cells in vitro. We analyze the cells using current fluorescent imaging techniques including spinning disk confocal, TIRF imaging and electron microscopy for high resolution understanding of morphological events at the cell surface. We are interested in how macrophages take up bacteria and destroy them intracellularly. We are also studying how some pathogens like Chlamydia trachomatis manipulate host cells to gain a bacterial advantage. We study how bone formation and bone loss is accomplished by osteoblasts and osteoclasts, respectively. Of interest we study how osteoarthritis , rheumatoid arthritis and the extreme effects of a microgravity environment influence the bone cells to cause net bone loss. Our work to date has been funded by NSERC, CIHR, CIHR New Emerging Team and Canadian Space Agency grants. Our laboratory is within the Science Wing at the University of Toronto Scarborough. As a member of the Centre for the Neurobiology of Stress facility, we have access to state-of-the-art equipment facilities and services, including cell culture facilities, imaging facilities (including confocal microscopy, scanning and transmission electron microscopy) and flow cytometry facilities, etc. The CNS is the result of a $10 million CFI award to UTSC and all of my trainees have full-time access to this facility.

 

Publications

2014

Top
Modulation of osteoclastogenesis with macrophage m1- and m2-inducing stimuli.Jeganathan S, Fiorino C, Naik U, Sun HS, Harrison RE.PLoS ONE 2014;9(8):e104498
pubmed

2013

Top
EB1 levels are elevated in ascorbic Acid (AA)-stimulated osteoblasts and mediate cell-cell adhesion-induced osteoblast differentiation.Pustylnik S, Fiorino C, Nabavi N, Zappitelli T, da Silva R, Aubin JE, Harrison RE.J. Biol. Chem. 2013 Jul;288(30):22096-110
pubmed
Monocytes from patients with osteoarthritis display increased osteoclastogenesis and bone resorption: the In Vitro Osteoclast Differentiation in Arthritis study.Durand M, Komarova SV, Bhargava A, Trebec-Reynolds DP, Li K, Fiorino C, Maria O, Nabavi N, Manolson MF, Harrison RE, Dixon SJ, Sims SM, Mizianty MJ, Kurgan L, Haroun S, Boire G, de Fatima Lucena-Fernandes M, de Brum-Fernandes AJ.Arthritis Rheum. 2013 Jan;65(1):148-58
pubmed

2012

Top
Rab GTPase mediated procollagen trafficking in ascorbic acid stimulated osteoblasts.Nabavi N, Pustylnik S, Harrison RE.PLoS ONE 2012;7(9):e46265
pubmed
Chlamydia trachomatis vacuole maturation in infected macrophages.Sun HS, Eng EW, Jeganathan S, Sin AT, Patel PC, Gracey E, Inman RD, Terebiznik MR, Harrison RE.J. Leukoc. Biol. 2012 Oct;92(4):815-27
pubmed
Classically activated macrophages use stable microtubules for matrix metalloproteinase-9 (MMP-9) secretion.Hanania R, Sun HS, Xu K, Pustylnik S, Jeganathan S, Harrison RE.J. Biol. Chem. 2012 Mar;287(11):8468-83
pubmed

2011

Top
Chlamydia trachomatis inclusions induce asymmetric cleavage furrow formation and ingression failure in host cells.Sun HS, Wilde A, Harrison RE.Mol. Cell. Biol. 2011 Dec;31(24):5011-22
pubmed
Effects of microgravity on osteoclast bone resorption and osteoblast cytoskeletal organization and adhesion.Nabavi N, Khandani A, Camirand A, Harrison RE.Bone 2011 Nov;49(5):965-74
pubmed
Measuring immune receptor mobility by fluorescence recovery after photobleaching.Silver K, Harrison RE.Methods Mol. Biol. 2011;748:155-67
pubmed
Kinesin 5B is necessary for delivery of membrane and receptors during FcγR-mediated phagocytosis.Silver KE, Harrison RE.J. Immunol. 2011 Jan;186(2):816-25
pubmed

2010

Top
Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets.Flannagan RS, Harrison RE, Yip CM, Jaqaman K, Grinstein S.J. Cell Biol. 2010 Dec;191(6):1205-18
pubmed

2009

Top
Localized diacylglycerol-dependent stimulation of Ras and Rap1 during phagocytosis.Botelho RJ, Harrison RE, Stone JC, Hancock JF, Philips MR, Jongstra-Bilen J, Mason D, Plumb J, Gold MR, Grinstein S.J. Biol. Chem. 2009 Oct;284(42):28522-32
pubmed
Proteomic analysis of microtubule-associated proteins during macrophage activation.Patel PC, Fisher KH, Yang EC, Deane CM, Harrison RE.Mol. Cell Proteomics 2009 Nov;8(11):2500-14
pubmed

2008

Top
Membrane ruffles capture C3bi-opsonized particles in activated macrophages.Patel PC, Harrison RE.Mol. Biol. Cell 2008 Nov;19(11):4628-39
pubmed
Lysosome dispersion in osteoblasts accommodates enhanced collagen production during differentiation.Nabavi N, Urukova Y, Cardelli M, Aubin JE, Harrison RE.J. Biol. Chem. 2008 Jul;283(28):19678-90
pubmed

2007

Top
Cytoplasmic linker protein-170 enhances spreading and phagocytosis in activated macrophages by stabilizing microtubules.Binker MG, Zhao DY, Pang SJ, Harrison RE.J. Immunol. 2007 Sep;179(6):3780-91
pubmed
Microtubules regulate PI-3K activity and recruitment to the phagocytic cup during Fcgamma receptor-mediated phagocytosis in nonelicited macrophages.Khandani A, Eng E, Jongstra-Bilen J, Schreiber AD, Douda D, Samavarchi-Tehrani P, Harrison RE.J. Leukoc. Biol. 2007 Aug;82(2):417-28
pubmed
MTOC reorientation occurs during FcgammaR-mediated phagocytosis in macrophages.Eng EW, Bettio A, Ibrahim J, Harrison RE.Mol. Biol. Cell 2007 Jul;18(7):2389-99
pubmed

2005

Top
Quantitative and dynamic assessment of the contribution of the ER to phagosome formation.Touret N, Paroutis P, Terebiznik M, Harrison RE, Trombetta S, Pypaert M, Chow A, Jiang A, Shaw J, Yip C, Moore HP, van der Wel N, Houben D, Peters PJ, de Chastellier C, Mellman I, Grinstein S.Cell 2005 Oct;123(1):157-70
pubmed

2004

Top
Salmonella impairs RILP recruitment to Rab7 during maturation of invasion vacuoles.Harrison RE, Brumell JH, Khandani A, Bucci C, Scott CC, Jiang X, Finlay BB, Grinstein S.Mol. Biol. Cell 2004 Jul;15(7):3146-54
pubmed
Acquisition of Hrs, an essential component of phagosomal maturation, is impaired by mycobacteria.Vieira OV, Harrison RE, Scott CC, Stenmark H, Alexander D, Liu J, Gruenberg J, Schreiber AD, Grinstein S.Mol. Cell. Biol. 2004 May;24(10):4593-604
pubmed
Leukocyte-specific protein 1 targets the ERK/MAP kinase scaffold protein KSR and MEK1 and ERK2 to the actin cytoskeleton.Harrison RE, Sikorski BA, Jongstra J.J. Cell. Sci. 2004 Apr;117(Pt 10):2151-7
pubmed

2003

Top
Phagocytosis by neutrophils.Lee WL, Harrison RE, Grinstein S.Microbes Infect. 2003 Nov;5(14):1299-306
pubmed
Phagosomes fuse with late endosomes and/or lysosomes by extension of membrane protrusions along microtubules: role of Rab7 and RILP.Harrison RE, Bucci C, Vieira OV, Schroer TA, Grinstein S.Mol. Cell. Biol. 2003 Sep;23(18):6494-506
pubmed
Modulation of Rab5 and Rab7 recruitment to phagosomes by phosphatidylinositol 3-kinase.Vieira OV, Bucci C, Harrison RE, Trimble WS, Lanzetti L, Gruenberg J, Schreiber AD, Stahl PD, Grinstein S.Mol. Cell. Biol. 2003 Apr;23(7):2501-14
pubmed

2002

Top
Phagocytosis and the microtubule cytoskeleton.Harrison RE, Grinstein S.Biochem. Cell Biol. 2002;80(5):509-15
pubmed
Microbial killing: oxidants, proteases and ions.Harrison RE, Touret N, Grinstein S.Curr. Biol. 2002 May;12(10):R357-9
pubmed