Rongmin Zhao

faculty_img Academic Title: Associate Professor

Campus: UTSC

CSB Appointment: Full

Primary Undergraduate Department:
Biological Sciences, UTSC

Graduate Programs:
Cell & Systems Biology

Titles and Honors:


Academic or Administrative Appointments:


Education:
Ph.D. Chinese Academy of Agricultural Sciences 1995, BSc, Peking University 1991

 

Mailing Address
Department of Cell & Systems Biology
University of Toronto
1265 Military Trail
Scarborough, ON M1C 1A4
Canada
 

 

Contact Information
Office phone: 416-208-2740 
Office: SY 248 
Lab: SY 215 
Lab phone: 416-208-2741 
Email: rzhao@utsc.utoronto.ca  
URL: http://www.utsc.utoronto.ca/~rzhao/

 

Research Areas
Developmental Biology
Molecular Biology
Physiology
Plant Biology
Proteomics
Systems Biology

 

Research

Protein homeostatic processes include protein synthesis, folding, stabilization of conformation, refolding of misfolded proteins and clearance of misfolded proteins inside the cell. To maintain a proper cellular protein homeostasis is important for all living organisms during normal growth and under stress conditions. My laboratory is interested in the mechanisms of different cellular protein quality control systems that are players for protein homeostasis. Specifically, we are interested in the mechanism of action of HSP90 family molecular chaperones and investigate how HSP90 proteins are involved in plant development, organelle function, and stress resistance by using Arabidopsis as a model organism. We use molecular, biochemical, proteomic, and genetic approaches to study the role of HSP90s and their interacting partners in Arabidopsis. Our research focuses on how HSP90 helps its client proteins to achieve their proper physiological functions during organelle biogenesis and normal physiological conditions. Additionally, we are interested in the mechanisms of regulated cellular protein degradation pathways. We use biochemical, genetic and proteomic approaches to study how certain proteins are selectively degraded by the 26S proteasome in a ubiquitin-independent pathway, by using baking yeast as a model organism.

 

Publications

2015

Top
A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis.Zhang M, Wang C, Lin Q, Liu A, Wang T, Feng X, Liu J, Han H, Ma Y, Bonea D, Zhao R, Hua X.Plant J. 2015 Aug;83(4):582-599
pubmed
A highly charged region in the middle domain of plant endoplasmic reticulum (ER)-localized heat-shock protein 90 is required for resistance to tunicamycin or high calcium-induced ER stresses.Chong LP, Wang Y, Gad N, Anderson N, Shah B, Zhao R.J. Exp. Bot. 2015 Jan;66(1):113-24
pubmed

2014

Top
Cosuppression of the chloroplast localized molecular chaperone HSP90.5 impairs plant development and chloroplast biogenesis in Arabidopsis.Oh SE, Yeung C, Babaei-Rad R, Zhao R.BMC Res Notes 2014;7:643
pubmed

2012

Top
The stability of the small nucleolar ribonucleoprotein (snoRNP) assembly protein Pih1 in Saccharomyces cerevisiae is modulated by its C terminus.Paci A, Liu XH, Huang H, Lim A, Houry WA, Zhao R.J. Biol. Chem. 2012 Dec;287(52):43205-14
pubmed
Structure of minimal tetratricopeptide repeat domain protein Tah1 reveals mechanism of its interaction with Pih1 and Hsp90.Jiménez B, Ugwu F, Zhao R, Ortí L, Makhnevych T, Pineda-Lucena A, Houry WA.J. Biol. Chem. 2012 Feb;287(8):5698-709
pubmed

2010

Top
Expression of five AtHsp90 genes in Saccharomyces cerevisiae reveals functional differences of AtHsp90s under abiotic stresses.Song H, Fan P, Shi W, Zhao R, Li Y.J. Plant Physiol. 2010 Sep;167(14):1172-8
pubmed
Tamoxifen enhances the Hsp90 molecular chaperone ATPase activity.Zhao R, Leung E, Grüner S, Schapira M, Houry WA.PLoS ONE 2010;5(4):e9934
pubmed

2009

Top
Overexpression of AtHsp90.2, AtHsp90.5 and AtHsp90.7 in Arabidopsis thaliana enhances plant sensitivity to salt and drought stresses.Song H, Zhao R, Fan P, Wang X, Chen X, Li Y.Planta 2009 Mar;229(4):955-64
pubmed

2008

Top
Molecular chaperone Hsp90 stabilizes Pih1/Nop17 to maintain R2TP complex activity that regulates snoRNA accumulation.Zhao R, Kakihara Y, Gribun A, Huen J, Yang G, Khanna M, Costanzo M, Brost RL, Boone C, Hughes TR, Yip CM, Houry WA.J. Cell Biol. 2008 Feb;180(3):563-78
pubmed

2007

Top
Expression of a constitutively activated plasma membrane H+-ATPase alters plant development and increases salt tolerance.Gévaudant F, Duby G, von Stedingk E, Zhao R, Morsomme P, Boutry M.Plant Physiol. 2007 Aug;144(4):1763-76
pubmed
Molecular interaction network of the Hsp90 chaperone system.Zhao R, Houry WA.Adv. Exp. Med. Biol. 2007;594:27-36
pubmed

2006

Top
Specificity in substrate and cofactor recognition by the N-terminal domain of the chaperone ClpX.Thibault G, Yudin J, Wong P, Tsitrin V, Sprangers R, Zhao R, Houry WA.Proc. Natl. Acad. Sci. U.S.A. 2006 Nov;103(47):17724-9
pubmed

2005

Top
Hsp90: a chaperone for protein folding and gene regulation.Zhao R, Houry WA.Biochem. Cell Biol. 2005 Dec;83(6):703-10
pubmed
Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone.Zhao R, Davey M, Hsu YC, Kaplanek P, Tong A, Parsons AB, Krogan N, Cagney G, Mai D, Greenblatt J, Boone C, Emili A, Houry WA.Cell 2005 Mar;120(5):715-27
pubmed