Stephen Tobe

faculty_img Academic TitleProfessor Emeritus

Campus: St. George

CSB Appointment: Full

Primary Undergraduate Department:
Cell & Systems Biology

Graduate Programs:
Cell & Systems Biology
Ecology & Evolutionary Biology

Titles and Honors:
Fellow of the Royal Society of Canada

Academic or Administrative Appointments
Education:
Ph.D. McGill University 1972
M.Sc. York University 1969
B.Sc. Queen’s University 1967

 

Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Harbord St.
Toronto, ON M5S 3G5
Canada

 

Contact Information
Office phone: 416-978-3517
Office: RW 601B
Email: stephen.tobe@utoronto.ca
URL: http://labs.csb.utoronto.ca/tobe

 

Research Areas
Animal Biology
Neurobiology
Physiology

 

Research

The Tobe lab studies various questions in arthropod endocrinology. Prof. Tobe’s primary interest is in the regulation of juvenile hormone production by neuropeptides called allatostatins. We are also interested in how the biosynthesis of juvenile hormone is regulated. We work on a variety of arthropods, particularly cockroaches but more recently, lobster, shrimp and crab in addition to Drosophila. Juvenile hormone is a principal regulator of reproduction in most insect species. Crustaceans seem to use some immediate precursors of juvenile hormone and we are interested in the role of juvenile hormone or precursors in egg production of adults.

 

Publications

2015

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How Did Arthropod Sesquiterpenoids and Ecdysteroids Arise? Comparison of Hormonal Pathway Genes in Noninsect Arthropod Genomes.Qu Z, Kenny NJ, Lam HM, Chan TF, Chu KH, Bendena WG, Tobe SS, Hui JH.Genome Biol Evol 2015 Jul;7(7):1951-9
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Evolution of Ecdysis and Metamorphosis in Arthropods: The Rise of Regulation of Juvenile Hormone.Cheong SP, Huang J, Bendena WG, Tobe SS, Hui JH.Integr. Comp. Biol. 2015 Nov;55(5):878-90
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RXR/USP and EcR are critical for the regulation of reproduction and the control of JH biosynthesis in Diploptera punctata.Hult EF, Huang J, Marchal E, Lam J, Tobe SS.J. Insect Physiol. 2015 Sep;80:48-60
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Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria.Lismont E, Vleugels R, Marchal E, Badisco L, Van Wielendaele P, Lenaerts C, Zels S, Tobe SS, Vanden Broeck J, Verlinden H.Front Neurosci 2015;9:84
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Methyl farnesoate plays a dual role in regulating Drosophila metamorphosis.Wen D, Rivera-Perez C, Abdou M, Jia Q, He Q, Liu X, Zyaan O, Xu J, Bendena WG, Tobe SS, Noriega FG, Palli SR, Wang J, Li S.PLoS Genet. 2015 Mar;11(3):e1005038
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Synthesis, biological activity, and conformational study of N-methylated allatostatin analogues inhibiting juvenile hormone biosynthesis.Xie Y, Zhang L, Zhang C, Wu X, Deng X, Yang X, Tobe SS.J. Agric. Food Chem. 2015 Mar;63(11):2870-6
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Characterization of the juvenile hormone pathway in the viviparous cockroach, Diploptera punctata.Huang J, Marchal E, Hult EF, Tobe SS.PLoS ONE 2015;10(2):e0117291
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Identification and characterization of the NMDA receptor and its role in regulating reproduction in the cockroach Diploptera punctata.Huang J, Hult EF, Marchal E, Tobe SS.J. Exp. Biol. 2015 Apr;218(Pt 7):983-90
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2014

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Mode of action of allatostatins in the regulation of juvenile hormone biosynthesis in the cockroach, Diploptera punctata.Huang J, Marchal E, Hult EF, Zels S, Vanden Broeck J, Tobe SS.Insect Biochem. Mol. Biol. 2014 Nov;54:61-8
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Methoprene-tolerant (Met) knockdown in the adult female cockroach, Diploptera punctata completely inhibits ovarian development.Marchal E, Hult EF, Huang J, Pang Z, Stay B, Tobe SS.PLoS ONE 2014;9(9):e106737
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Identification of putative ecdysteroid and juvenile hormone pathway genes in the shrimp Neocaridina denticulata.Sin YW, Kenny NJ, Qu Z, Chan KW, Chan KW, Cheong SP, Leung RW, Chan TF, Bendena WG, Chu KH, Tobe SS, Hui JH.Gen. Comp. Endocrinol. 2014 Aug;
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Probing the active conformation of FGLamide allatostatin analogs with N-terminal modifications using NMR spectroscopy and molecular modeling.Xie Y, Zhang L, Wu XQ, Zhang CL, Yang XL, Tobe SS.Peptides 2014 Jul;
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In vivo crystallography at X-ray free-electron lasers: the next generation of structural biology?.Gallat FX, Matsugaki N, Coussens NP, Yagi KJ, Boudes M, Higashi T, Tsuji D, Tatano Y, Suzuki M, Mizohata E, Tono K, Joti Y, Kameshima T, Park J, Song C, Hatsui T, Yabashi M, Nango E, Itoh K, Coulibaly F, Tobe S, Ramaswamy S, Stay B, Iwata S, Chavas LM.Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2014 Jul;369(1647):20130497
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Genomic sequence and experimental tractability of a new decapod shrimp model, Neocaridina denticulata.Kenny NJ, Sin YW, Shen X, Zhe Q, Wang W, Chan TF, Tobe SS, Shimeld SM, Chu KH, Hui JH.Mar Drugs 2014 Mar;12(3):1419-37
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2013

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Sequencing and validation of housekeeping genes for quantitative real-time PCR during the gonadotrophic cycle of Diploptera punctata.Marchal E, Hult EF, Huang J, Tobe SS.BMC Res Notes 2013;6:237
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Diploptera punctata as a model for studying the endocrinology of arthropod reproduction and development.Marchal E, Hult EF, Huang J, Stay B, Tobe SS.Gen. Comp. Endocrinol. 2013 Jul;188:85-93
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How are comparative genomics and the study of microRNAs changing our views on arthropod endocrinology and adaptations to the environment?.Kenny NJ, Quah S, Holland PW, Tobe SS, Hui JH.Gen. Comp. Endocrinol. 2013 Jul;188:16-22
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2012

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Molecular characterization and gene expression of juvenile hormone binding protein in the bamboo borer, Omphisa fuscidentalis.Ritdachyeng E, Manaboon M, Tobe SS, Singtripop T.J. Insect Physiol. 2012 Nov;58(11):1493-501
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Select Neuropeptides and their G-Protein Coupled Receptors in Caenorhabditis Elegans and Drosophila Melanogaster.Bendena WG, Campbell J, Zara L, Tobe SS, Chin-Sang ID.Front Endocrinol (Lausanne) 2012;3:93
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Defining the contribution of select neuropeptides and their receptors in regulating sesquiterpenoid biosynthesis by Drosophila melanogaster ring gland/corpus allatum through RNAi analysis.Wang C, Zhang J, Tobe SS, Bendena WG.Gen. Comp. Endocrinol. 2012 May;176(3):347-53
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Farnesoic acid and methyl farnesoate production during lobster reproduction: possible functional correlation with retinoid X receptor expression.Tiu SH, Hult EF, Yagi KJ, Tobe SS.Gen. Comp. Endocrinol. 2012 Jan;175(2):259-69
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2011

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Leucomyosuppressin modulates cardiac rhythm in the cockroach Blattella germanica.Maestro JL, Tobe SS, Belles X.J. Insect Physiol. 2011 Dec;57(12):1677-81
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Molecular evolution of ultraspiracle protein (USP/RXR) in insects.Hult EF, Tobe SS, Chang BS.PLoS ONE 2011;6(8):e23416
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Evidence for differential biosynthesis of juvenile hormone (and related) sesquiterpenoids in Drosophila melanogaster.Bendena WG, Zhang J, Burtenshaw SM, Tobe SS.Gen. Comp. Endocrinol. 2011 May;172(1):56-61
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Peptidomimetics in the discovery of new insect growth regulators: studies on the structure-activity relationships of the core pentapeptide region of allatostatins.Kai ZP, Xie Y, Huang J, Tobe SS, Zhang JR, Ling Y, Zhang L, Zhao YC, Yang XL.J. Agric. Food Chem. 2011 Mar;59(6):2478-85
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Final steps in juvenile hormone biosynthesis in the desert locust, Schistocerca gregaria.Marchal E, Zhang J, Badisco L, Verlinden H, Hult EF, Van Wielendaele P, Yagi KJ, Tobe SS, Vanden Broeck J.Insect Biochem. Mol. Biol. 2011 Apr;41(4):219-27
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Design, synthesis and biological activity of peptidomimetic analogs of insect allatostatins.Xie Y, Kai ZP, Tobe SS, Deng XL, Ling Y, Wu XQ, Huang J, Zhang L, Yang XL.Peptides 2011 Mar;32(3):581-6
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Influence of codon usage bias on FGLamide-allatostatin mRNA secondary structure.Martínez-Pérez F, Bendena WG, Chang BS, Tobe SS.Peptides 2011 Mar;32(3):509-17
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2010

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Comparative genomic and phylogenetic analysis of vitellogenin and other large lipid transfer proteins in metazoans.Hayward A, Takahashi T, Bendena WG, Tobe SS, Hui JH.FEBS Lett. 2010 Mar;584(6):1273-8
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Synthesis, biological activity, and hologram quantitative structure-activity relationships of novel allatostatin analogues.Kai ZP, Huang J, Xie Y, Tobe SS, Ling Y, Zhang L, Zhao YC, Yang XL.J. Agric. Food Chem. 2010 Mar;58(5):2652-8
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The effects of farnesoic acid and 20-hydroxyecdysone on vitellogenin gene expression in the lobster, Homarus americanus, and possible roles in the reproductive process.Tiu SH, Chan SM, Tobe SS.Gen. Comp. Endocrinol. 2010 Apr;166(2):337-45
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Evolution and functional divergence of enzymes involved in sesquiterpenoid hormone biosynthesis in crustaceans and insects.Hui JH, Hayward A, Bendena WG, Takahashi T, Tobe SS.Peptides 2010 Mar;31(3):451-5
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2009

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Suppression of JH biosynthesis by JH analog treatment: mechanism of suppression and roles of allatostatins and nervous connections in the cockroach Diploptera punctata.Lenkic LE, Tiu SH, Tobe SS.J. Insect Physiol. 2009 Nov;55(11):967-75
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FGLamide Allatostatin genes in Arthropoda: introns early or late?.Martínez-Pérez F, Bendena WG, Chang BS, Tobe SS.Peptides 2009 Jul;30(7):1241-8
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A potential insect growth regulator: synthesis and bioactivity of an allatostatin mimic.Kai ZP, Huang J, Tobe SS, Yang XL.Peptides 2009 Jul;30(7):1249-53
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Cloning and expression study of the lobster (Homarus americanus) vitellogenin: Conservation in gene structure among decapods.Tiu SH, Hui HL, Tsukimura B, Tobe SS, He JG, Chan SM.Gen. Comp. Endocrinol. 2009 Jan;160(1):36-46
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Effects of selected neuropeptides, mating status and castration on male reproductive tract movements and immunolocalization of neuropeptides in earwigs.Rankin SM, TeBrugge VA, Murray JA, Schuler AM, Tobe SS.Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 2009 Jan;152(1):83-90
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The synthesis and biological activity of linear and cyclic analogs of the two diuretic peptides of Diploptera punctata.Kaskani C, Poulos CP, Zhang J, Tobe SS.Peptides 2009 Mar;30(3):603-7
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2008

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Quantification of allatostatin receptor mRNA levels in the cockroach, Diploptera punctata, using real-time PCR.Lungchukiet P, Zhang J, Tobe SS, Bendena WG.J. Insect Physiol. 2008 Jun;54(6):981-7
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Reconstruction of ancestral FGLamide-type insect allatostatins: a novel approach to the study of allatostatin function and evolution.Hult EF, Weadick CJ, Chang BS, Tobe SS.J. Insect Physiol. 2008 Jun;54(6):959-68
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Endocrine and reproductive differences and genetic divergence in two populations of the cockroach Diploptera punctata.Lenkic LE, Wolfe JM, Chang BS, Tobe SS.J. Insect Physiol. 2008 Jun;54(6):931-8
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Molecular cloning and characterization of an allatostatin-like receptor in the cockroach Diploptera punctata.Lungchukiet P, Donly BC, Zhang J, Tobe SS, Bendena WG.Peptides 2008 Feb;29(2):276-85
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Characterization of the putative farnesoic acid O-methyltransferase (LvFAMeT) cDNA from white shrimp, Litopenaeus vannamei: Evidence for its role in molting.Hui JH, Tobe SS, Chan SM.Peptides 2008 Feb;29(2):252-60
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A Caenorhabditis elegans allatostatin/galanin-like receptor NPR-9 inhibits local search behavior in response to feeding cues.Bendena WG, Boudreau JR, Papanicolaou T, Maltby M, Tobe SS, Chin-Sang ID.Proc. Natl. Acad. Sci. U.S.A. 2008 Jan;105(4):1339-42
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2007

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The role of neuropeptides in caterpillar nutritional ecology.Bede JC, McNeil JN, Tobe SS.Peptides 2007 Jan;28(1):185-96
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The role of allatostatins in juvenile hormone synthesis in insects and crustaceans.Stay B, Tobe SS.Annu. Rev. Entomol. 2007;52:277-99
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2006

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Characterization of vitellogenin in the shrimp Metapenaeus ensis: expression studies and hormonal regulation of MeVg1 transcription in vitro.Tiu SH, Hui JH, He JG, Tobe SS, Chan SM.Mol. Reprod. Dev. 2006 Apr;73(4):424-36
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Hemolymph clotting in crustaceans: Implications for neuropeptide extraction from invertebrate hemolymph.Kwok R, Tobe SS.Peptides 2006 Mar;27(3):590-6
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Bioinformatic analysis of neuropeptide and receptor expression profiles during midgut metamorphosis in Drosophila melanogaster.Bowser PR, Tobe SS.Peptides 2006 Mar;27(3):583-9
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2005

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Effects of Manduca allatotropin and localization of Manduca allatotropin-immunoreactive cells in earwigs.Rankin SM, Kwok R, Seymour ML, Shaon Rahman U, Tobe SS.Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 2005 Sep;142(1):113-22
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Regulation of methyl farnesoate production by mandibular organs in the crayfish, Procambarus clarkii: a possible role for allatostatins.Kwok R, Rui Zhang J, Tobe SS.J. Insect Physiol. 2005 Apr;51(4):367-78
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Immunocytochemical analysis of putative allatostatin receptor (DAR-2) distribution in the CNS of larval Drosophila melanogaster.Bowser PR, Tobe SS.Peptides 2005 Jan;26(1):81-7
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Vitellogenesis in the red crab Charybdis feriatus: Hepatopancreas-specific expression and farnesoic acid stimulation of vitellogenin gene expression.Mak AS, Choi CL, Tiu SH, Hui JH, He JG, Tobe SS, Chan SM.Mol. Reprod. Dev. 2005 Mar;70(3):288-300
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2004

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Purification and characterization of a mandibular organ protein from the American lobster, Homarus americanus: a putative farnesoic acid O-methyltransferase.Holford KC, Edwards KA, Bendena WG, Tobe SS, Wang Z, Borst DW.Insect Biochem. Mol. Biol. 2004 Aug;34(8):785-98
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2003

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Immunolocalization of allatostatin-like neuropeptides and their putative receptor in eyestalks of the tiger prawn, Penaeus monodon.Panchan N, Bendena WG, Bowser P, Lungchukiet P, Tobe SS, Sithigorngul W, Chaivisuthangkura P, Rangsiruji A, Petsom A, Pewnim T, Sithigorngul P.Peptides 2003 Oct;24(10):1563-70
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The distribution and effects of Dippu-allatostatin-like peptides in the blood-feeding bug, Rhodnius prolixus.Sarkar NR, Tobe SS, Orchard I.Peptides 2003 Oct;24(10):1553-62
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Crustacean neuropeptide genes of the CHH/MIH/GIH family: implications from molecular studies.Chan SM, Gu PL, Chu KH, Tobe SS.Gen. Comp. Endocrinol. 2003 Dec;134(3):214-9
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Quantification and visualization of Dippu-AST mRNA in the brain of adult Diploptera punctata: mated females vs. virgin females vs. males.Garside CS, Bendena WG, Tobe SS.J. Insect Physiol. 2003 Apr;49(4):285-91
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2002

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Myotropic peptides in Drosophila melanogaster and the genes that encode them.Nichols R, Bendena WG, Tobe SS.J. Neurogenet. 2002 Jan-Mar;16(1):1-28
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Drosophila melanogaster flatline encodes a myotropin orthologue to Manduca sexta allatostatin.Price MD, Merte J, Nichols R, Koladich PM, Tobe SS, Bendena WG.Peptides 2002 Apr;23(4):787-94
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