George Espie

faculty_img Academic Title: Professor

Campus: UTM

CSB Appointment: Full

Primary Undergraduate Department:
Biology, UTM

Graduate Programs:
Cell & Systems Biology

Titles and Honors:


Academic or Administrative Appointments:


Education:
Ph.D. York University 1985
B.Sc. York University 1979

 

Mailing Address
Department of Cell & Systems Biology
University of Toronto
3359 Mississauga Road North
Mississauga, ON L5L 1C6
Canada
 

 

Contact Information
Office phone: 905-828-5380 
Office: DV 4053 
Lab: DV 4028 
Lab phone: 905-828-5219 
Email: george.espie@utoronto.ca 
URL: LAB

 

Research Areas
Biotechnology
Cell Biology
Microbiology
Molecular Biology
Physiology
Plant Biology
Structural Biology

 

Research


Microbial physiology, molecular biology, biochemistry and photosynthesis. Current research is directed towards: identifying the molecular components essential to the CO2 – concentrating mechanism of aquatic, photosynthetic microorganisms; defining the role of carbonic anhydrase in photosynthesis; acquiring an understanding of the biochemical mechanisms involved in the active transport HCO3-; regulation of expression of the CO2/HCO3- transport systems and the control of photosynthetic electron transport by CO2/HCO3-.Using molecular and biochemical tools, we are also analysing the structure, assembly and protein targeting to carboxysomes, the unique CO2 fixing organelles of cyanobacteria. Also, in collaboration with colleagues in chemical engineering, we are using our expertise in microbial photosynthesis to investigate the industrial use of microalgal biofilms grown in photobioreactors as a source for biofuels and a means for waste water remediation.

 

Publications

2017
2016
2015
2014
2013
2012
2011
2010
2009
2008
2007
2006
2005
2004
2003
2002
 

2016

Top

The structure, kinetics and interactions of the β-carboxysomal β-carbonic anhydrase, CcaA McGurn, LD, M Moazami-Goudarzi, SA White, T Suwal, B Brar, JQ Tang, GS Espie & MS Kimber 2016 Biochemical Journal 473: 4559 – 4572
pubmed
Improved biomass productivity in algal biofilms through synergistic interactions between photon flux density and CO2 concentration Schnurr, PJ, O Molenda, E Edwards GS Espie & DG Allen 2016 Bioresource Technology 219: 72 – 79
pubmed
The effect of photon flux density on algal biofilm growth and internal fatty acid concentrations Schnurr, P, GS Espie & DG Allen 2016 Algal Research 16: 349 -356
DOI: 10.1016/j.algal.2016.04.001
 

2015

Top

Ratiometric fluorescence transduction by hybridization after isothermal amplification for determination of zeptomole quantities of oligonucleotide biomarkers with a paper-based platform and camera-based detection Noor, MO, D Hrovata, M. Moazami-Goudarzi, GS Espie and UJ. Krull 2015 Analytica Chimica Acta 885: 156–165
pubmed
 

2014

Top

Identification and characterization a carboxysomal γ-carbonic anhydrase from the cyanobacterium Nostoc sp. PCC 7120. de Araujo, C, D Arefeen, Y Tadesse, B Long GD Price, RS Rowlett, MS Kimber & GS Espie 2014 Photosynthesis Research 121: 135-150
pubmed
The effect of light direction and suspended cell concentrations on algal biofilm growth rates, Schnurr, P., G.S. Espie, D.G. Allen 2014 Applied Microbiology and Biotechnology 98:8553–8562
pubmed
Organization of astaxanthin within lipid bodies of Haematococcus pluvialis studied with polarization-dependent harmonic generation microscopy. Tokarz, D.,R Cisek, O El-Ansari, GS Espie, U Fekl and V Barzda 2014 PLoS ONE 9(9): e107804.
pubmed
 

2013

Top

Algae biofilm growth and the potential to stimulate lipid accumulation through nutrient starvationSchnurr, P., G.S. Espie, D.G. Allen 2013. Bioresource Technology 136: 337–344
pubmed
Evolution of the biochemistry of the photorespiratory C2 cycle. Hagemann, M., A. Fernie, G.S. Espie, R. Kern, M. Eisenhut, S. Reumann, H. Bauwe and A.M. Weber 2013. Plant Biology 15: 639 – 647
pubmed
Engineering photorespiration: current state and future possibilities Peterhansel, C., K. Krause, H-P. Braun, G.S. Espie, A. R. Fernie, D. T. Hanson, O. Keech, V. G. Maurino, M. Mielewczik and R. F. Sage 2013 Plant Biology 15: 754 – 758
pubmed
 

2012

Top

Inactivation of a low temperature induced RNA helicase in Synechocystis sp. PCC6803: Physiological and morphological consequences Rosana, A.R.R., M. Ventakesh, D. Chamot, L.M. Patterson-Fortin, O. Tarassova, G.S. Espie and G.W. Owttrim 2012. Plant and Cell Physiology 53: 646–658
pubmed
 

2011

Top

Physiological characterization and light response of the CO2-concentrating mechanism in the filamentous cyanobacterium Leptolyngbya sp. CPCC 696 de Araujo, E.D., J. Patel, C. de Araujo, S. P. Rogers, S.M. Short, D.A. Campbell and G.S. Espie 2011. Photosynthesis Research 109: 85 – 101
pubmed
Carboxysomes: cyanobacterial RubisCO comes in small packages. Espie, G.S. and M.S. Kimber 2011 Photosynthesis Research 109: 7 – 20
pubmed
 

2010

Top

Structural basis of the oxidative activation of the carboxysomal γ-carbonic anhydrase, CcmMPena, KL., S.E. Castel, C. de Araujo, G.S. Espie and M.S. Kimber 2010. Proceeding of the National Academy of Sciences of the United States of America (PNAS) 107: 2455-2460
pubmed

2009

Top

Optical microscopy in photosynthesis.Cisek R, Spencer L, Prent N, Zigmantas D, Espie GS, Barzda V.Photosyn. Res. 2009 Nov-Dec;102(2-3):111-41
pubmed

2008

Top

A multiprotein bicarbonate dehydration complex essential to carboxysome function in cyanobacteria.Cot SS, So AK, Espie GS.J. Bacteriol. 2008 Feb;190(3):936-45
pubmed

2007

Top

Involvement of the cynABDS operon and the CO2-concentrating mechanism in the light-dependent transport and metabolism of cyanate by cyanobacteria.Espie GS, Jalali F, Tong T, Zacal NJ, So AK.J. Bacteriol. 2007 Feb;189(3):1013-24
pubmed

2006

Top

Mechanism of the down regulation of photosynthesis by blue light in the Cyanobacterium synechocystis sp. PCC 6803.Scott M, McCollum C, Vasil’ev S, Crozier C, Espie GS, Krol M, Huner NP, Bruce D.Biochemistry 2006 Jul;45(29):8952-8
pubmed

2004

Top

A novel evolutionary lineage of carbonic anhydrase (epsilon class) is a component of the carboxysome shell.So AK, Espie GS, Williams EB, Shively JM, Heinhorst S, Cannon GC.J. Bacteriol. 2004 Feb;186(3):623-30
pubmed

2002

Top

Mitochondrial-driven bicarbonate transport supports photosynthesis in a marine microalga.Huertas IE, Colman B, Espie GS.Plant Physiol. 2002 Sep;130(1):284-91
pubmed
The energy source for CO2 transport in the marine microalga Nannochloris atomus.Huertas IE, Espie GS, Colman B.Planta 2002 Apr;214(6):947-53
pubmed
Effects of carbon nutrition on the physiological expression of HCO3- transport and the CO2-concentrating mechanism in the Cyanobacterium chlorogloeopsis sp. ATCC 27193.Skleryk RS, So AK, Espie GS.Planta 2002 Feb;214(4):572-83
pubmed
Characterization of a mutant lacking carboxysomal carbonic anhydrase from the cyanobacterium Synechocystis PCC6803.So AK, John-McKay M, Espie GS.Planta 2002 Jan;214(3):456-67
pubmed