Michael Phillips

michael-phillips2 Academic Title: Assistant Professor

Campus: UTM (note: Currently recruiting PhD students)

CSB Appointment: Full

Primary Undergraduate Department:
Biology

Graduate Programs:
Cell & Systems Biology

Titles and Honors:Academic or Administrative Appointments:Education:
PhD Washington State University 2000
BSc California State University at Humboldt 1994
Mailing Address
Department of Biology
University of Toronto
3359 Mississauga Road
Mississauga, ON L5L 1C6
Canada
Contact Information
Office phone: 905-569-4848
Office: DV-4003
Lab: DV-4026
Lab phone:
Email: michaelandrew.phillips@utoronto.ca
URL: Plantmetabolism.blogspot.com
Research Areas

Chemical Biology
Cell Biology
Metabolomics
Molecular Biology
Plant Biology
Quantitative Biology
Systems Biology

Research
My research focuses on the control of flux in plant metabolic networks. This includes regulatory mechanisms which govern the division of fixed carbon between primary and secondary metabolic pathways as well as the adaptive responses of the plant metabolome to stress. Metabolic control analysis (MCA) is a mathematical framework which describes the distribution of control among the enzymes in a biosynthetic pathway, and my research uses direct, kinetic data obtained from whole plant isotopic labeling experiments to calculate MCA control coefficients of biosynthetic enzymes leading to isoprenoids and other high value plant metabolites. This quantitative description of plant metabolism relies on analytical techniques such as mass spectrometry and ultimately helps us understand the regulatory mechanisms controlling the flow of carbon through the complex web of metabolic pathways in plant cells. The response of plants to insect feeding stress, drought, or heat stress can be predicted and improved using the information produced in this research program. The factors controlling the biosynthesis of important biotechnological products derived from plants can also be identified to maximize the yield of valuable natural products, including medicines, biofuels, and essential vitamins in crops.
Publications

2019

Top
The plastidial metabolite 2-C-methyl-D-erythritol-2,4-cyclodiphosphate modulates defence responses against aphids.Onkokesung N, Reichelt M, Wright LP, Phillips MA, Gershenzon J, Dicke M. 2019. Plant, Cell & Environment.
doi

2018

Top
Nerolidol production in agroinfiltrated tobacco: Impact of protein stability and membrane targeting of strawberry (Fragraria ananassa) NEROLIDOL SYNTHASE1..Andrade P, Manzano D, Ramirez-Estrada K, Caudepon D, Arro M, Ferrer A, Phillips MA. Plant Sci. 2018 Feb; 267():112-123
pubmed

2017

Top
Non-invasive quantification of ethylene in attached fruit headspace at 1 p.p.b. by gas chromatography-mass spectrometry.Pereira L, Pujol M, Garcia-Mas J, Phillips MA. 2017. The Plant Journal.
doi

2016

Top
Differential Subplastidial Localization and Turnover of Enzymes Involved in Isoprenoid Biosynthesis in Chloroplasts..Perello C, Llamas E, Burlat V, Ortiz-Alcaide M, Phillips MA, Pulido P, Rodriguez-Concepcion M. PLoS ONE 2016; 11(2):e0150539
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2015

Top
Comparative transcriptional profiling analysis of developing melon (Cucumis melo L.) fruit from climacteric and non-climacteric varieties..Saladié M, Cañizares J, Phillips MA, Rodriguez-Concepcion M, Larrigaudière C, Gibon Y, Stitt M, Lunn JE, Garcia-Mas J. BMC Genomics 2015 Jun 09; 16():440
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The diversion of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate from the 2-C-methyl-D-erythritol 4-phosphate pathway to hemiterpene glycosides mediates stress responses in Arabidopsis thaliana..González-Cabanelas D, Wright LP, Paetz C, Onkokesung N, Gershenzon J, Rodríguez-Concepción M, Phillips MA. Plant J. 2015 Apr; 82(1):122-37
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2014

Top
The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps..Saladié M, Wright LP, Garcia-Mas J, Rodriguez-Concepcion M, Phillips MA. J. Exp. Bot. 2014 Sep; 65(17):5077-92
pubmed
Deoxyxylulose 5-Phosphate Synthase Controls Flux through the Methylerythritol 4-Phosphate Pathway in Arabidopsis..Wright LP, Rohwer JM, Ghirardo A, Hammerbacher A, Ortiz-Alcaide M, Raguschke B, Schnitzler JP, Gershenzon J, Phillips MA. Plant Physiol. 2014 Aug; 165(4):1488-1504
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Measuring the activity of 1-deoxy-D-xylulose 5-phosphate synthase, the first enzyme in the MEP pathway, in plant extracts..Wright LP, Phillips MA. Methods Mol. Biol. 2014; 1153():9-20
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Influence of medium and elicitors on the production of cocaine, amino acids and phytohormones by Erythroxylum coca calli.Docimo T, Davis AJ, Luck K, Fellenberg C, Reichelt M, Phillips M, Gershenzon J, D’Auria JC. 2014. Plant Cell, Tissue and Organ Culture (PCTOC).
doi

2013

Top
Arabidopsis J-protein J20 delivers the first enzyme of the plastidial isoprenoid pathway to protein quality control..Pulido P, Toledo-Ortiz G, Phillips MA, Wright LP, Rodríguez-Concepción M. Plant Cell 2013 Oct; 25(10):4183-94
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2012

Top
Triple subcellular targeting of isopentenyl diphosphate isomerases encoded by a single gene..Guirimand G, Guihur A, Phillips MA, Oudin A, Glévarec G, Mahroug S, Melin C, Papon N, Clastre M, Giglioli-Guivarc\'h N, St-Pierre B, Rodríguez-Concepción M, Burlat V, Courdavault V. Plant Signal Behav 2012 Nov; 7(11):1495-7
pubmed
A single gene encodes isopentenyl diphosphate isomerase isoforms targeted to plastids, mitochondria and peroxisomes in Catharanthus roseus..Guirimand G, Guihur A, Phillips MA, Oudin A, Glévarec G, Melin C, Papon N, Clastre M, St-Pierre B, Rodríguez-Concepción M, Burlat V, Courdavault V. Plant Mol. Biol. 2012 Jul; 79(4-5):443-59
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2010

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Pleiotropic regulatory locus 1 (PRL1) integrates the regulation of sugar responses with isoprenoid metabolism in Arabidopsis..Flores-Pérez U, Pérez-Gil J, Closa M, Wright LP, Botella-Pavía P, Phillips MA, Ferrer A, Gershenzon J, Rodríguez-Concepción M. Mol Plant 2010 Jan; 3(1):101-12
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2009

Top
Evaluation of Candidate Reference Genes for Real-Time Quantitative PCR of Plant Samples Using Purified cDNA as Template..Phillips MA, D\'Auria JC, Luck K, Gershenzon J. Plant Mol. Biol. Rep. 2009; 27():407-416
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Quantitative iTRAQ proteome and comparative transcriptome analysis of elicitor-induced Norway spruce (Picea abies) cells reveals elements of calcium signaling in the early conifer defense response..Lippert DN, Ralph SG, Phillips M, White R, Smith D, Hardie D, Gershenzon J, Ritland K, Borchers CH, Bohlmann J. Proteomics 2009 Jan; 9(2):350-67
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2008

Top
The plastidial MEP pathway: unified nomenclature and resources..Phillips MA, León P, Boronat A, Rodríguez-Concepción M. Trends Plant Sci. 2008 Dec; 13(12):619-23
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The Arabidopsis thaliana type I Isopentenyl Diphosphate Isomerases are targeted to multiple subcellular compartments and have overlapping functions in isoprenoid biosynthesis..Phillips MA, D\'Auria JC, Gershenzon J, Pichersky E. Plant Cell 2008 Mar; 20(3):677-96
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2007

Top
Functional identification and differential expression of 1-deoxy-D-xylulose 5-phosphate synthase in induced terpenoid resin formation of Norway spruce (Picea abies)..Phillips MA, Walter MH, Ralph SG, Dabrowska P, Luck K, Urós EM, Boland W, Strack D, Rodríguez-Concepción M, Bohlmann J, Gershenzon J. Plant Mol. Biol. 2007 Oct; 65(3):243-57
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2006

Top
Molecular Regulation of Induced Terpenoid Biosynthesis in Conifers.Phillips MA, Bohlmann J, Gershenzon J. 2006. Phytochemistry Reviews.
doi

2004

Top
Metabolic engineering of isoprenoid biosynthesis in Arabidopsis for the production of taxadiene, the first committed precursor of Taxol..Besumbes O, Sauret-Güeto S, Phillips MA, Imperial S, Rodríguez-Concepción M, Boronat A. Biotechnol. Bioeng. 2004 Oct 20; 88(2):168-75
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Regulation of carotenoid biosynthesis in plants: evidence for a key role of hydroxymethylbutenyl diphosphate reductase in controlling the supply of plastidial isoprenoid precursors..Botella-Pavía P, Besumbes O, Phillips MA, Carretero-Paulet L, Boronat A, Rodríguez-Concepción M. Plant J. 2004 Oct; 40(2):188-99
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Distinct light-mediated pathways regulate the biosynthesis and exchange of isoprenoid precursors during Arabidopsis seedling development..Rodríguez-Concepción M, Forés O, Martinez-García JF, González V, Phillips MA, Ferrer A, Boronat A. Plant Cell 2004 Jan; 16(1):144-56
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2003

Top
cDNA isolation, functional expression, and characterization of (+)-alpha-pinene synthase and (-)-alpha-pinene synthase from loblolly pine (Pinus taeda): stereocontrol in pinene biosynthesis..Phillips MA, Wildung MR, Williams DC, Hyatt DC, Croteau R. Arch. Biochem. Biophys. 2003 Mar 15; 411(2):267-76
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1999

Top
Monoterpene synthases of loblolly pine (Pinus taeda) produce pinene isomers and enantiomers..Phillips MA, Savage TJ, Croteau R. Arch. Biochem. Biophys. 1999 Dec 01; 372(1):197-204
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cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis)..Bohlmann J, Phillips M, Ramachandiran V, Katoh S, Croteau R. Arch. Biochem. Biophys. 1999 Aug 15; 368(2):232-43
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