Penney Gilbert

faculty_img Academic Title: Associate Professor

Campus: St. George

CSB Appointment: Cross Appointment

Primary Undergraduate Department: Institute of Biomaterials and Biomedical Engineering

Graduate Programs:
Cell and Systems Biology

Institute of Biomaterials and Biomedical Engineering

Academic or Administrative Appointments:
Advisor, Ontario Institute for Regenerative Medicine

Education:
PhD, University of Pennsylvania 2006
BSc, Haverford College 1999

Mailing Address
164 College Street,
Rosebrugh Building Rm. 407,
Toronto, Ontario,
M5S3G9.

 

Contact Information
Office phone: 416-978-2501
Office: CCBR 506
Lab phone: 416-978-2507
Lab: CCBR 540
Email: penney.gilbert@utoronto.ca
URL: www.gilbert-lab.com

 

Research Areas

Cell Biology

 

Research

Ah, the marvel of muscle! Your skeletal muscle tissue allows you to move, smile, and breathe. When you want to move, your brain sends signals via motor neurons to the neuromuscular junction where a burst of acetylcholine is released. This in turn actives a cascade of calcium within each skeletal muscle fiber invoking tiny molecular motors to crawl along cords of actin protein and produce a coordinated contraction of your muscle. As if this weren’t enough, when skeletal muscle is injured, muscle stem cells residing in the tissue are called to action to restore muscle form and function.
As we age, our ability to regenerate skeletal muscle declines and the tissue becomes weak. Loss of skeletal muscle mass and function also occurs as a result of certain genetic conditions, inactivity and diseases like cancer. The goal of our research is to restore muscle function in these diverse disease settings by harnessing the potential of muscle stem cells. To do this, we employ tools, methods, and materials developed by our engineering colleagues to better understand how the body controls muscle stem cell behavior and fate. We also engineer miniatured 3D models of skeletal muscle to model and study endogenous repair in a dish.

 

 

Publications

 

 

2018

Top

Muscle stem cell intramuscular delivery within hyaluronan methylcellulose improves engraftment efficiency and dispersion. Davoudi S, Chin CY, Cooke MJ, Tam RY, Shoichet MS, Gilbert PM. Biomaterials. 2018 Aug;173:34-46. doi: 10.1016/j.biomaterials.2018.04.048. Epub 2018 Apr 26.
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Cellular Biomechanics in Skeletal Muscle Regeneration. Li EW, McKee-Muir OC, Gilbert PM. Curr Top Dev Biol. 2018;126:125-176. doi: 10.1016/bs.ctdb.2017.08.007. Epub 2017 Oct 31.
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2017

Top

Optimization of Satellite Cell Culture Through Biomaterials. Davoudi S, Gilbert PM. Methods Mol Biol. 2017;1556:329-341. doi: 10.1007/978-1-4939-6771-1_18.pubmed
Stage-specific differences in secretory profile of mesenchymal stromal cells (MSCs) subjected to early- vs late-stage OA synovial fluid. Gómez-Aristizábal A, Sharma A, Bakooshli MA, Kapoor M, Gilbert PM, Viswanathan S, Gandhi R. Osteoarthritis Cartilage. 2017 May;25(5):737-741. doi: 10.1016/j.joca.2016.11.010. Epub 2016 Nov 25.pubmed
Cellular adaptation to biomechanical stress across length scales in tissue homeostasis and disease. Gilbert PM, Weaver VM. Semin Cell Dev Biol. 2017 Jul;67:141-152. doi: 10.1016/j.semcdb.2016.09.004. Epub 2016 Sep 15.pubmed
 

2016

Top

Biomechanical Origins of Muscle Stem Cell Signal Transduction. Morrissey JB, Cheng RY, Davoudi S, Gilbert PM. J Mol Biol. 2016 Apr 10;428(7):1441-54. doi: 10.1016/j.jmb.2015.05.004. Epub 2015 May 21.pubmed
 

2015

Top

Muscling in on the third dimension. Afshar Bakooshli M, Gilbert PM. Elife. 2015 Feb 16;4:e06430. doi: 10.7554/eLife.06430.
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Global linking of cell tracks using the Viterbi algorithm. Magnusson KE, Jalden J, Gilbert PM, Blau HM. IEEE Trans Med Imaging. 2015 Apr;34(4):911-29. doi: 10.1109/TMI.2014.2370951. Epub 2014 Nov 14.pubmed
 

2014

Top

Tissue mechanics modulate microRNA-dependent PTEN expression to regulate malignant progression. Mouw JK, Yui Y, Damiano L, Bainer RO, Lakins JN, Acerbi I, Ou G, Wijekoon AC, Levental KR, Gilbert PM, Hwang ES, Chen YY, Weaver VM. Nat Med. 2014 Apr;20(4):360-7. doi: 10.1038/nm.3497. Epub 2014 Mar 16.
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Rejuvenation of the muscle stem cell population restores strength to injured aged muscles. Cosgrove BD, Gilbert PM, Porpiglia E, Mourkioti F, Lee SP, Corbel SY, Llewellyn ME, Delp SL, Blau HM. Nat Med. 2014 Mar;20(3):255-64. doi: 10.1038/nm.3464. Epub 2014 Feb 16.
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2012

Top

Protein-engineered biomaterials to generate human skeletal muscle mimics. Sengupta D, Gilbert PM, Johnson KJ, Blau HM, Heilshorn SC. Adv Healthc Mater. 2012 Nov;1(6):785-9. doi: 10.1002/adhm.201200195. Epub 2012 Sep 5.pubmed
A single cell bioengineering approach to elucidate mechanisms of adult stem cell self-renewal. Gilbert PM, Corbel S, Doyonnas R, Havenstrite K, Magnusson KE, Blau HM. Integr Biol (Camb). 2012 Apr;4(4):360-7. doi: 10.1039/c2ib00148a. Epub 2012 Feb 10.
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2011

Top

Single-cell phospho-specific flow cytometric analysis demonstrates biochemical and functional heterogeneity in human hematopoietic stem and progenitor compartments. Gibbs KD Jr, Gilbert PM, Sachs K, Zhao F, Blau HM, Weissman IL, Nolan GP, Majeti R. Blood. 2011 Apr 21;117(16):4226-33. doi: 10.1182/blood-2010-07-298232. Epub 2011 Feb 28.
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Engineering a stem cell house into a home. Gilbert PM, Blau HM. Stem Cell Res Ther. 2011 Jan 31;2(1):3. doi: 10.1186/scrt44.
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2010

Top

Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture. Gilbert PM, Havenstrite KL, Magnusson KE, Sacco A, Leonardi NA, Kraft P, Nguyen NK, Thrun S, Lutolf MP, Blau HM. Science. 2010 Aug 27;329(5995):1078-81. doi: 10.1126/science.1191035. Epub 2010 Jul 15.
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HOXA9 regulates BRCA1 expression to modulate human breast tumor phenotype. Gilbert PM, Mouw JK, Unger MA, Lakins JN, Gbegnon MK, Clemmer VB, Benezra M, Licht JD, Boudreau NJ, Tsai KK, Welm AL, Feldman MD, Weber BL, Weaver VM. J Clin Invest. 2010 May;120(5):1535-50. doi: 10.1172/JCI39534. Epub 2010 Apr 12.
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2009

Top

Designing materials to direct stem-cell fate. Lutolf MP, Gilbert PM, Blau HM. Nature. 2009 Nov 26;462(7272):433-41. doi: 10.1038/nature08602.pubmed
A home away from home: challenges and opportunities in engineering in vitro muscle satellite cell niches. Cosgrove BD, Sacco A, Gilbert PM, Blau HM. Differentiation. 2009 Sep-Oct;78(2-3):185-94. doi: 10.1016/j.diff.2009.08.004.
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2005

Top

Saccharomyces cerevisiae Rab-GDI displacement factor ortholog Yip3p forms distinct complexes with the Ypt1 Rab GTPase and the reticulon Rtn1p.Geng J, Shin ME, Gilbert PM, Collins RN, Burd CG. Eukaryot Cell. 2005 Jul;4(7):1166-74.
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2004

Top

Watch thy neighbor: cancer is a communal affair. Weaver VM, Gilbert P. J Cell Sci. 2004 Mar 15;117(Pt 8):1287-90.
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2003

Top

Dual prenylation is required for Rab protein localization and function. Calero M, Chen CZ, Zhu W, Winand N, Havas KA, Gilbert PM, Burd CG, Collins RN. Mol Biol Cell. 2003 May;14(5):1852-67. Epub 2003 Feb 6.
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2001

Top

FYVE domain targets Pib1p ubiquitin ligase to endosome and vacuolar membranes. Shin ME, Ogburn KD, Varban OA, Gilbert PM, Burd CG. J Biol Chem. 2001 Nov 2;276(44):41388-93. Epub 2001 Aug 28.
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GDP dissociation inhibitor domain II required for Rab GTPase recycling. Gilbert PM, Burd CG. J Biol Chem. 2001 Mar 16;276(11):8014-20. Epub 2000 Dec 14.
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