Ho-Sung Rhee

faculty_img Academic TitleAssistant Professor

Campus: UTM

CSB Appointment: Full

Primary Undergraduate Department:
Biology

Graduate Programs:
Cell and Systems Biology
Neuroscience
Developmental Biology

Titles and Honors:
Howard Hughes Medical Institute Postdoctoral Fellow (2014-2017)
Connaught Scholar (2018-2020)

Academic or Administrative Appointments:


Education:
Ph.D. Pennsylvania State University 2011
M.Sc. Seoul National University 2006
B.Sc. Sogang University 2004

 

Mailing Address

Department of Cell and Systems Biology
University of Toronto
3359 Mississauga Road
Mississauga, Ontario L5L 1C6
Canada

 

Contact Information
Office phone: 905-569-4931
Office: DV 3045
Lab: DV 1083
Lab phone: 905-828-3751
Email: hosung.rhee@utoronto.ca
URL:http://sites.utm.utoronto.ca/rhee/

 

Research Areas

Cell Biology
Molecular Biology
Systems Biology
Neurobiology

 

Research
 


In the mammalian body, there are thousands of cell types, which have the same genomic DNA in the nucleus of each cell. Genetically identical cells express a distinct set of genes during embryonic development, resulting in a variety of cell types. Our research focuses on understanding how a unique neuronal cell identity is established and maintained during mammalian development. This knowledge is key to understanding neurodevelopment and modelling neurodegenerative diseases. We study the molecular bases of global gene regulatory networks involved in neural development and disease.

To understand these processes, we utilize molecular, cellular, and epigenetics approaches combined with cutting-edge genomic techniques and stem cell differentiation methods. For example, we developed a novel genomic mapping method, called ChIP-exo, to detect the genomic binding locations of proteins (Cell, 2011). Furthermore, by using efficient embryonic stem cell differentiation and genome engineering systems (Neuron, 2016), we are studying how neuronal transcription factors control gene expression during motor neuron development. Our expertise in these comprehensive approaches provides greater detail and insight into molecular mechanisms of cell type-specific gene expression programs in the mammalian central nervous system.

 

Publications

 

 

2016

Top

Expression of Terminal Effector Genes in Mammalian Neurons Is Maintained by a Dynamic Relay of Transient Enhancers. Rhee HS, Closser M, Guo Y, Bashkirova EV, Tan GC, Gifford DK, Wichterle H. Neuron. 2016 Dec 21;92(6):1252-1265. doi: 10.1016/j.neuron.2016.11.037. Epub 2016 Dec 8.
pubmed
 

2014

Top

Subnucleosomal structures and nucleosome asymmetry across a genome. Rhee HS, Bataille AR, Zhang L, Pugh BF. Cell. 2014 Dec 4;159(6):1377-88. doi: 10.1016/j.cell.2014.10.054.
pubmed
A comprehensive and high-resolution genome-wide response of p53 to stress.Chang GS, Chen XA, Park B, Rhee HS, Li P, Han KH, Mishra T, Chan-Salis KY, Li Y, Hardison RC, Wang Y, Pugh BF. Cell Rep. 2014 Jul 24;8(2):514-27. doi: 10.1016/j.celrep.2014.06.030. Epub 2014 Jul 17.
pubmed
 

2013

Top

Kinetic competition between elongation rate and binding of NELF controls promoter-proximal pausing. Li J, Liu Y, Rhee HS, Ghosh SK, Bai L, Pugh BF, Gilmour DS. Mol Cell. 2013 Jun 6;50(5):711-22. doi: 10.1016/j.molcel.2013.05.016.
pubmed
 

2012

Top

ChIP-exo method for identifying genomic location of DNA-binding proteins with near-single-nucleotide accuracy. Rhee HS, Pugh BF. Curr Protoc Mol Biol. 2012 Oct;Chapter 21:Unit 21.24. doi: 10.1002/0471142727.mb2124s100.
pubmed
Genome-wide structure and organization of eukaryotic pre-initiation complexes. Rhee HS, Pugh BF. Nature. 2012 Jan 18;483(7389):295-301. doi: 10.1038/nature10799.
pubmed
 

2011

Top

Comprehensive genome-wide protein-DNA interactions detected at single-nucleotide resolution. Rhee HS, Pugh BF.(N Y) Cell. 2011 Dec 9;147(6):1408-19. doi: 10.1016/j.cell.2011.11.013.
pubmed
 

2009

Top

Interaction of transcriptional regulators with specific nucleosomes across the Saccharomyces genome. Koerber RT, Rhee HS, Jiang C, Pugh BF.Mol Cell. 2009 Sep 24;35(6):889-902. doi: 10.1016/j.molcel.2009.09.011.
pubmed
 

2006

Top

Fatty acids, inhibitors for the DNA binding of c-Myc/Max dimer, suppress proliferation and induce apoptosis of differentiated HL-60 human leukemia cell.Jung KC, Park CH, Hwang YH, Rhee HS, Lee JH, Kim HK, Yang CH. Leukemia. 2006 Jan;20(1):122-7.
pubmed
 

2005

Top

Momordin I, an inhibitor of AP-1, suppressed osteoclastogenesis through inhibition of NF-kappaB and AP-1 and also reduced osteoclast activity and survival. Hwang YH, Lee JW, Hahm ER, Jung KC, Lee JH, Park CH, Rhee HS, Ryu JM, Kim HK, Yang CH. Biochem Biophys Res Commun. 2005 Nov 25;337(3):815-23. Epub 2005 Sep 28.
pubmed
Negative regulation of beta-catenin/Tcf signaling by naringenin in AGS gastric cancer cell. Lee JH, Park CH, Jung KC, Rhee HS, Yang CH. Biochem Biophys Res Commun. 2005 Sep 30;335(3):771-6.
pubmed
Determination of the dissociation constants for recombinant c-Myc, Max, and DNA complexes: the inhibitory effect of linoleic acid on the DNA-binding step. Jung KC, Rhee HS, Park CH, Yang CH. Biochem Biophys Res Commun. 2005 Aug 19;334(1):269-75.
pubmed
Ionomycin downregulates beta-catenin/Tcf signaling in colon cancer cell line. Park CH, Hahm ER, Lee JH, Jung KC, Rhee HS, Yang CH. Carcinogenesis. 2005 Nov;26(11):1929-33. Epub 2005 Jun 1.
pubmed