Ho-Sung Rhee

faculty_img Academic TitleAssistant Professor

Campus: UTM

CSB Appointment: Full

Primary Undergraduate Department:
Biology

Graduate Programs:
Cell & Systems Biology

Titles and Honors:Academic or Administrative Appointments:

Education:
Ph.D. Pennsylvania State University 2011
M.Sc. Seoul National University 2006
B.Sc. Sogang University 2004

 

Mailing Address

Department of Cell and Systems Biology
University of Toronto
3359 Mississauga Road
Mississauga, Ontario L5L 1C6
Canada

 

Contact Information
Office phone: 905-569-4931
Office: DV 3045
Lab: DV 3045
Lab phone:
Email: hosung.rhee@utoronto.ca
URL:

 

Research Areas

Molecular Biology
Functional Biology
Cell Biology
Neurobiology

 

Research
 

Research in our laboratory focuses on understanding how genes are controlled and a unique neuronal cell identity is established during embryonic development. The acquisition of cell fate is driven by a cell type-specific gene expression program activated by programming transcription factors (TFs). Nearly every gene is packaged into chromatin by an array of nuclear proteins in a tiny nucleus of the cell. The recruitment of TFs to their target genes is accompanied with profound chromatin regulation. The genomic targets and functions of nuclear proteins in pluripotent cells have been identified. However, it is largely unknown how chromatin organization impacts the recruitment of programming TFs to their target genes, controlling specification of postmitotic neuronal cell identity. It results in challenges of stem cell differentiation into specific neurons for disease modeling and regenerative medicine. To understand these processes, we utilize cutting-edge stem cell programming and systems biology approaches. Previously, we developed a novel genomic mapping method, called ChIP-exo, to detect the genomic binding locations of proteins at near base-pair resolution (Rhee and Pugh, Cell. 2011). Furthermore, by using efficient stem cell differentiation and genome engineering systems, we revealed how the programming TFs control gene expression programs during motor neuron development (Rhee et al., Neuron. 2016). Our expertise in these comprehensive approaches provides greater detail and insight into chromatin regulatory mechanisms of cell type-specific gene expression programs in the mammalian central nervous system. The long-term goal of our laboratory is to dissect the global gene regulatory networks involved in neural development and disease.

 

Publications

 


2016
2014
2013
2012
2011
2009
2007
2006
2005
 

2016

Top

Expression of Terminal Effector Genes in Mammalian Neurons Is Maintained by a Dynamic Relay of Transient Enhancers. Rhee HS, Closser M, Guo Y, Bashkirova EV, Tan GC, Gifford DK, Wichterle H. Neuron. 2016 Dec 21;92(6):1252-1265. doi: 10.1016/j.neuron.2016.11.037. Epub 2016 Dec 8.
pubmed
 

2014

Top

Subnucleosomal structures and nucleosome asymmetry across a genome. Rhee HS, Bataille AR, Zhang L, Pugh BF. Cell. 2014 Dec 4;159(6):1377-88. doi: 10.1016/j.cell.2014.10.054.
pubmed
A comprehensive and high-resolution genome-wide response of p53 to stress.Chang GS, Chen XA, Park B, Rhee HS, Li P, Han KH, Mishra T, Chan-Salis KY, Li Y, Hardison RC, Wang Y, Pugh BF. Cell Rep. 2014 Jul 24;8(2):514-27. doi: 10.1016/j.celrep.2014.06.030. Epub 2014 Jul 17.
pubmed
 

2013

Top

Kinetic competition between elongation rate and binding of NELF controls promoter-proximal pausing. Li J, Liu Y, Rhee HS, Ghosh SK, Bai L, Pugh BF, Gilmour DS. Mol Cell. 2013 Jun 6;50(5):711-22. doi: 10.1016/j.molcel.2013.05.016.
pubmed
 

2012

Top

ChIP-exo method for identifying genomic location of DNA-binding proteins with near-single-nucleotide accuracy. Rhee HS, Pugh BF. Curr Protoc Mol Biol. 2012 Oct;Chapter 21:Unit 21.24. doi: 10.1002/0471142727.mb2124s100.
pubmed
Genome-wide structure and organization of eukaryotic pre-initiation complexes. Rhee HS, Pugh BF. Nature. 2012 Jan 18;483(7389):295-301. doi: 10.1038/nature10799.
pubmed
 

2011

Top

Comprehensive genome-wide protein-DNA interactions detected at single-nucleotide resolution. Rhee HS, Pugh BF.(N Y) Cell. 2011 Dec 9;147(6):1408-19. doi: 10.1016/j.cell.2011.11.013.
pubmed
 

2009

Top

Interaction of transcriptional regulators with specific nucleosomes across the Saccharomyces genome. Koerber RT, Rhee HS, Jiang C, Pugh BF.Mol Cell. 2009 Sep 24;35(6):889-902. doi: 10.1016/j.molcel.2009.09.011.
pubmed
 

2006

Top

Fatty acids, inhibitors for the DNA binding of c-Myc/Max dimer, suppress proliferation and induce apoptosis of differentiated HL-60 human leukemia cell.Jung KC, Park CH, Hwang YH, Rhee HS, Lee JH, Kim HK, Yang CH. Leukemia. 2006 Jan;20(1):122-7.
pubmed
 

2005

Top

Momordin I, an inhibitor of AP-1, suppressed osteoclastogenesis through inhibition of NF-kappaB and AP-1 and also reduced osteoclast activity and survival. Hwang YH, Lee JW, Hahm ER, Jung KC, Lee JH, Park CH, Rhee HS, Ryu JM, Kim HK, Yang CH. Biochem Biophys Res Commun. 2005 Nov 25;337(3):815-23. Epub 2005 Sep 28.
pubmed
Negative regulation of beta-catenin/Tcf signaling by naringenin in AGS gastric cancer cell. Lee JH, Park CH, Jung KC, Rhee HS, Yang CH. Biochem Biophys Res Commun. 2005 Sep 30;335(3):771-6.
pubmed
Determination of the dissociation constants for recombinant c-Myc, Max, and DNA complexes: the inhibitory effect of linoleic acid on the DNA-binding step. Jung KC, Rhee HS, Park CH, Yang CH. Biochem Biophys Res Commun. 2005 Aug 19;334(1):269-75.
pubmed
Ionomycin downregulates beta-catenin/Tcf signaling in colon cancer cell line. Park CH, Hahm ER, Lee JH, Jung KC, Rhee HS, Yang CH. Carcinogenesis. 2005 Nov;26(11):1929-33. Epub 2005 Jun 1.
pubmed