Tony Harris

faculty_img Academic Title: Professor

Campus: St. George

CSB Appointment: Full

Primary Undergraduate Department:
Cell & Systems Biology

Graduate Programs:
Cell & Systems Biology

Titles and Honors:
Canada Research Chair in Cell Polarity and Animal Development (2005-2015)
Member, CIHR College of Reviewers
Member, Faculty of 1000 (Pattern formation section)

Academic or Administrative Appointments:
Associate Chair for Undergraduate Studies

Education:
Ph.D. Toronto 2003
B.Sc., Western 1996

 

Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Harbord St.
Toronto, ON M5S 3G5
Canada

 

Contact Information
Office phone: 416-946-0873
Office: RW 529C
Lab: RW 529 / 531
Lab phone: 416-946-7225
Email: tony.harris@utoronto.ca
URL: https://sites.google.com/site/harrislabutoronto/home

 

Research Areas
Cell Biology
Developmental Biology
Molecular Biology
Systems Biology

 

Research
We study the molecular bases of how animal cells and tissues are built. We use confocal and time-lapse microscopy in combination with Drosophila genetics and molecular biology to study how epithelial cells are formed and re-shaped during development. Epithelia are sheets of adherent cells that form boundaries between our body compartments. Each side of an epithelial sheet has distinct molecular properties. This epithelial polarity is critical for guiding embryo development and directing adult functions (e.g. nutrient uptake in the gut), and its loss is associated with cancer. We are focusing on the molecular machinery responsible for forming epithelial cells (polarity, adhesion, cytoskeletal and membrane trafficking complexes). We are identifying the proteins making up this machinery and determining how they function together in short-range and long-range circuits to establish, maintain and remodel epithelial structure in the Drosophila embryo.

 

I enjoy working on difficult problems with trainees who are excited about cell biology.  People with backgrounds in molecular/cellular/developmental biology are encouraged to contact me, as are those with backgrounds in mathematical simulations interested in applying this approach to cell biology.  Please email a statement of interest, your CV and a copy of your academic record.

 

Publications

 

2017

Top

An Actomyosin-Arf-GEF Negative Feedback Loop for Tissue Elongation under Stress. West JJ, Zulueta-Coarasa T, Maier JA, Lee DM1, Bruce AEE, Fernandez-Gonzalez R, Harris TJC. Curr Biol. 2017 Aug 7;27(15):2260-2270.e5. doi: 10.1016/j.cub.2017.06.038. Epub 2017 Jul 20.
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2016

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The Arf GAP Asap promotes Arf1 function at the Golgi for cleavage furrow biosynthesis in Drosophila.Rodrigues, Francisco F, Wei Shao, and Tony J C Harris. 2016. Molecular biology of the cell, no. 20 ( 17).
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Cadherin trafficking for tissue morphogenesis: control and consequences. West, Junior J, and Tony J C Harris. 2016. Traffic (Copenhagen, Denmark) ( 23). doi:10.1111/tra.12407.
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2015

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PH Domain-Arf G Protein Interactions Localize the Arf-GEF Steppke for Cleavage Furrow Regulation in Drosophila.Lee DM, Rodrigues FF, Yu CG, Swan M, Harris TJ.PLoS ONE 2015;10(11):e0142562
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A Par-1-Par-3-Centrosome Cell Polarity Pathway and Its Tuning for Isotropic Cell Adhesion.Jiang T, McKinley RF, McGill MA, Angers S, Harris TJ.Curr. Biol. 2015 Oct;25(20):2701-8
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Polarized E-cadherin endocytosis directs actomyosin remodeling during embryonic wound repair.Hunter MV, Lee DM, Harris TJ, Fernandez-Gonzalez R.J. Cell Biol. 2015 Aug;210(5):801-16
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Germ Cell Segregation from the Drosophila Soma Is Controlled by an Inhibitory Threshold Set by the Arf-GEF Steppke.Lee DM, Wilk R, Hu J, Krause HM, Harris TJ.Genetics 2015 Jul;200(3):863-72
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Stepping stone: a cytohesin adaptor for membrane cytoskeleton restraint in the syncytial Drosophila embryo.Liu J, Lee DM, Yu CG, Angers S, Harris TJ.Mol. Biol. Cell 2015 Feb;26(4):711-25
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2014

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Coordinating the cytoskeleton and endocytosis for regulated plasma membrane growth in the early Drosophila embryo.Lee DM, Harris TJ.Bioarchitecture 2014 Mar-Apr;4(2):68-74
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Peptide binding properties of the three PDZ domains of Bazooka (Drosophila Par-3).Yu CG, Tonikian R, Felsensteiner C, Jhingree JR, Desveaux D, Sidhu SS, Harris TJ.PLoS ONE 2014;9(1):e86412
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2013

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Adherens junction distribution mechanisms during cell-cell contact elongation in Drosophila.Goldenberg G, Harris TJ.PLoS ONE 2013;8(11):e79613
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Bazooka inhibits aPKC to limit antagonism of actomyosin networks during amnioserosa apical constriction.David DJ, Wang Q, Feng JJ, Harris TJ.Development 2013 Dec;140(23):4719-29
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An Arf-GEF regulates antagonism between endocytosis and the cytoskeleton for Drosophila blastoderm development.Lee DM, Harris TJ.Curr. Biol. 2013 Nov;23(21):2110-20
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2012

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Displacement of basolateral Bazooka/PAR-3 by regulated transport and dispersion during epithelial polarization in Drosophila.McKinley RF, Harris TJ.Mol. Biol. Cell 2012 Nov;23(22):4465-71
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Interactions between the PDZ domains of Bazooka (Par-3) and phosphatidic acid: in vitro characterization and role in epithelial development.Yu CG, Harris TJ.Mol. Biol. Cell 2012 Sep;23(18):3743-53
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An introduction to adherens junctions: from molecular mechanisms to tissue development and disease.Harris TJ.Subcell. Biochem. 2012;60:1-5
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Assembly of Bazooka polarity landmarks through a multifaceted membrane-association mechanism.McKinley RF, Yu CG, Harris TJ.J. Cell. Sci. 2012 Mar;125(Pt 5):1177-90
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Adherens junction assembly and function in the Drosophila embryo.Harris TJ.Int Rev Cell Mol Biol 2012;293:45-83
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Live imaging of Drosophila embryos: quantifying protein numbers and dynamics at subcellular locations.David DJ, McGill MA, McKinley RF, Harris TJ.Methods Mol. Biol. 2012;839:1-17
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2010

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Adherens junctions: from molecules to morphogenesis.Harris TJ, Tepass U.Nat. Rev. Mol. Cell Biol. 2010 Jul;11(7):502-14
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The PAR complex regulates pulsed actomyosin contractions during amnioserosa apical constriction in Drosophila.David DJ, Tishkina A, Harris TJ.Development 2010 May;137(10):1645-55
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A modifier screen for Bazooka/PAR-3 interacting genes in the Drosophila embryo epithelium.Shao W, Wu J, Chen J, Lee DM, Tishkina A, Harris TJ.PLoS ONE 2010;5(4):e9938
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2009

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How the cytoskeleton helps build the embryonic body plan: models of morphogenesis from Drosophila.Harris TJ, Sawyer JK, Peifer M.Curr. Top. Dev. Biol. 2009;89:55-85
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Independent cadherin-catenin and Bazooka clusters interact to assemble adherens junctions.McGill MA, McKinley RF, Harris TJ.J. Cell Biol. 2009 Jun;185(5):787-96
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2008

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Control of cell flattening and junctional remodeling during squamous epithelial morphogenesis in Drosophila.Pope KL, Harris TJ.Development 2008 Jul;135(13):2227-38
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2007

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aPKC controls microtubule organization to balance adherens junction symmetry and planar polarity during development.Harris TJ, Peifer M.Dev. Cell 2007 May;12(5):727-38
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2005

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The positioning and segregation of apical cues during epithelial polarity establishment in Drosophila.Harris TJ, Peifer M.J. Cell Biol. 2005 Aug;170(5):813-23
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Decisions, decisions: beta-catenin chooses between adhesion and transcription.Harris TJ, Peifer M.Trends Cell Biol. 2005 May;15(5):234-7
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2004

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Regulation of cell-cell adhesion during Dictyostelium development.Siu CH, Harris TJ, Wang J, Wong E.Semin. Cell Dev. Biol. 2004 Dec;15(6):633-41
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Adherens junction-dependent and -independent steps in the establishment of epithelial cell polarity in Drosophila.Harris TJ, Peifer M.J. Cell Biol. 2004 Oct;167(1):135-47
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2003

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Cytoskeleton interactions involved in the assembly and function of glycoprotein-80 adhesion complexes in dictyostelium.Harris TJ, Ravandi A, Awrey DE, Siu CH.J. Biol. Chem. 2003 Jan;278(4):2614-23
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2002

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Reciprocal raft-receptor interactions and the assembly of adhesion complexes.Harris TJ, Siu CH.Bioessays 2002 Nov;24(11):996-1003
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