|Department of Cell and Systems Biology
University of Toronto,
25 Harbord Street,
Animal development is breathtaking process whereby a single cell gives rise to all of the body’s specialized cells and tissues. How would you reverse engineer this process to re-create tissue if it’s lost or damaged? That’s exactly what happens during limb regeneration in salamanders – an assortment of complex tissues must produce and organize progenitors to recapitulate the re-development of lost structures. What makes this even more impressive is that unlike the embryo, that has a stereotypical limb size, regenerative processes are robust enough to adapt to changes in the size and position of the missing limb piece. Our primary research aim is to understand how cell intrinsic and microenvironmental extrinsic signals orchestrate cells and tissue to perfectly reconstruct missing tissue. We combine live imaging of transgenic axolotls with ex vivo cell biology and molecular biology to understand the principles that underlie tissue regeneration and apply what we learn to mouse and human models with the goal of improving regeneration and wound healing. The Currie lab is launching this summer and is recruiting trainees at all levels. Please contact Josh about opportunities to be a part of our research.
|Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration.Gerber T, Murawala P, Knapp D, Masselink W, Schuez M, Hermann S, Gac-Santel M, Nowoshilow S, Kageyama J, Khattak S, Currie JD, Camp JG, Tanaka EM, Treutlein B. Science. 2018 Oct 26;362(6413). pii: eaaq0681. doi: 10.1126/science.aaq0681. Epub 2018 Sep 27.
|Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage. Kaucka M, Zikmund T, Tesarova M, Gyllborg D, Hellander A, Jaros J, Kaiser J, Petersen J, Szarowska B, Newton PT, Dyachuk V, Li L, Qian H, Johansson AS, Mishina Y, Currie JD, Tanaka EM, Erickson A, Dudley A, Brismar H, Southam P, Coen E, Chen M, Weinstein LS, Hampl A, Arenas E, Chagin AS, Fried K, Adameyko I. Elife. 2017 Apr 17;6. pii: e25902. doi: 10.7554/eLife.25902.
|Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools. Currie JD, Kawaguchi A, Traspas RM, Schuez M, Chara O, Tanaka EM. Dev Cell. 2016 Nov 21;39(4):411-423. doi: 10.1016/j.devcel.2016.10.013. Epub 2016 Nov 10.
|Regeneration: the ultimate example of wound healing. Murawala P, Tanaka EM, Currie JD. Semin Cell Dev Biol. 2012 Dec;23(9):954-62. doi: 10.1016/j.semcdb.2012.09.013. Epub 2012 Oct 8.