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PhD Transfer Exam- Kaitlin Montanera (Rhee lab)
January 30 @ 12:30 pm - 1:00 pm
PhD Transfer Exam
Thursday, January 30th, 2020 at 11:30am – MN-6128, University of Toronto, Mississauga
Kaitlin Montanera (Rhee lab)
“Role of Neuron-Specific Chromatin Remodelling Factor, CREST, in Spinal Motor Neurons”
Over one meter of DNA is packaged into the nucleus of eukaryotic cells, in the form of chromatin, to accommodate a large amount of genetic information. ATP-dependent chromatin remodellers are major contributors to changes in chromatin structure leading to cell fate specification. ATP-dependent chromatin-remodeller Brg/Brm-associated factor (BAF) complexes have specific subunits required for gene expression of post-mitotic neurons. CREST is a calcium-dependent transcriptional coactivator that is a dedicated, nonexchangeable and functional subunit of neuronal BAF complexes. By creating CREST knockout cell lines and using an efficient mouse stem cell differentiation method, I aim to examine the role of CREST in spinal motor neurons. I aim to determine the genomic mechanisms by which CREST is recruited to regulatory regions and its impact on the maintenance of neuronal gene expression. This study will provide novel insight into how distinct chromatin remodelling subunits play a role in neuron-specific gene expression.