Tony Harris

faculty_img
Academic Title: Professor

Campus: St. George

CSB Appointment: Full

Primary Undergraduate Department:
Cell & Systems Biology

Graduate Programs:
Cell & Systems Biology

Titles and Honors:
Canada Research Chair in Cell Polarity and Animal Development (2005-2015)
Member, CIHR College of Reviewers
Member, Faculty of 1000 (Pattern formation section)

Academic or Administrative Appointments:
Associate Chair for Undergraduate Studies

 

Education:
Ph.D. Toronto 2003
B.Sc., Western 1996

Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Harbord St.
Toronto, ON M5S 3G5
Canada
Contact Information
Office phone: 416-946-0873
Office: RW 517A/518
Lab: RW 507
Lab phone: 416-946-7225
Email: tony.harris@utoronto.ca
URL: http://harris.csb.utoronto.ca
Research Areas
Cell Biology
Developmental Biology
Molecular Biology
Systems Biology
Research
My lab pursues how cells and tissues are built.  Using the Drosophila embryo as a model system, our core approach is to manipulate molecules with genetics and to visualize effects on molecular complexes, cells and tissues using spinning disk confocal microscopy.  We investigate how cytoskeletal networks form and function to shape cells and tissues, studying specific molecular regulators of these networks as well as how distinct networks impact each other, the cell cortex, and cell-cell adhesion complexes for multicellular development.  We study cell polarity (how pathways direct assembly of molecular materials to particular locations of the cell), self-organization (how molecular control circuits amplify or downregulate complex formation), and emergent, multi-scale effects (how the positioning and activities of molecular materials induce physical changes to whole cells or tissues).  Our fundamental research is relevant to human diseases, regenerative medicine, bioengineering, and materials science.

 

I enjoy working on difficult problems with trainees who are fascinated about fundamental mechanisms of cell biology.  People with backgrounds in molecular/cellular/developmental biology are encouraged to contact me, as are those with backgrounds in mathematical simulations interested in applying this approach to cell biology.  Please email a statement of interest, your CV and a copy of your academic record.

Publications

2019

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Arf-GEF localization and function at myosin-rich adherens junctions via coiled-coil heterodimerization with an adaptor protein..Zheng S, West JJ, Yu CG, Harris TJC. Mol. Biol. Cell 2019 Dec 15; 30(26):3090-3103
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Par-1 controls the composition and growth of cortical actin caps during embryo cleavage..Jiang T, Harris TJC. J. Cell Biol. 2019 Dec 02; 218(12):4195-4214
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Apical polarity proteins recruit the RhoGEF Cysts to promote junctional myosin assembly..Silver JT, Wirtz-Peitz F, Simões S, Pellikka M, Yan D, Binari R, Nishimura T, Li Y, Harris TJC, Perrimon N, Tepass U. J. Cell Biol. 2019 Oct 07; 218(10):3397-3414
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Key roles of Arf small G proteins and biosynthetic trafficking for animal development..Rodrigues FF, Harris TJC. Small GTPases 2019 11; 10(6):403-410
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2018

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Dynamics of PAR Proteins Explain the Oscillation and Ratcheting Mechanisms in Dorsal Closure..Durney CH, Harris TJC, Feng JJ. Biophys. J. 2018 12 04; 115(11):2230-2241
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Collision of Expanding Actin Caps with Actomyosin Borders for Cortical Bending and Mitotic Rounding in a Syncytium..Zhang Y, Yu JC, Jiang T, Fernandez-Gonzalez R, Harris TJC. Dev. Cell 2018 06 04; 45(5):551-564.e4
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Organizing Complex Tissue Architecture by Pushing and Pulling Cell Contacts..Zhang Y, Harris TJC. Dev. Cell 2018 02 26; 44(4):407-409
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Sculpting epithelia with planar polarized actomyosin networks: Principles from Drosophila..Harris TJC. Semin. Cell Dev. Biol. 2018 09; 81():54-61
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2017

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Protein clustering for cell polarity: Par-3 as a paradigm..Harris TJC. F1000Res 2017; 6():1620
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An Actomyosin-Arf-GEF Negative Feedback Loop for Tissue Elongation under Stress..West JJ, Zulueta-Coarasa T, Maier JA, Lee DM, Bruce AEE, Fernandez-Gonzalez R, Harris TJC. Curr. Biol. 2017 Aug 07; 27(15):2260-2270.e5
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2016

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The Arf GAP Asap promotes Arf1 function at the Golgi for cleavage furrow biosynthesis in Drosophila..Rodrigues FF, Shao W, Harris TJ. Mol. Biol. Cell 2016 10 15; 27(20):3143-3155
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Cadherin Trafficking for Tissue Morphogenesis: Control and Consequences..West JJ, Harris TJ. Traffic 2016 12; 17(12):1233-1243
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2015

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PH Domain-Arf G Protein Interactions Localize the Arf-GEF Steppke for Cleavage Furrow Regulation in Drosophila.Lee DM, Rodrigues FF, Yu CG, Swan M, Harris TJ. PLoS ONE 2015;10(11):e0142562
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A Par-1-Par-3-Centrosome Cell Polarity Pathway and Its Tuning for Isotropic Cell Adhesion.Jiang T, McKinley RF, McGill MA, Angers S, Harris TJ. Curr. Biol. 2015 Oct;25(20):2701-8
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Polarized E-cadherin endocytosis directs actomyosin remodeling during embryonic wound repair.Hunter MV, Lee DM, Harris TJ, Fernandez-Gonzalez R. J. Cell Biol. 2015 Aug;210(5):801-16
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Germ Cell Segregation from the Drosophila Soma Is Controlled by an Inhibitory Threshold Set by the Arf-GEF Steppke.Lee DM, Wilk R, Hu J, Krause HM, Harris TJ. Genetics 2015 Jul;200(3):863-72
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Stepping stone: a cytohesin adaptor for membrane cytoskeleton restraint in the syncytial Drosophila embryo.Liu J, Lee DM, Yu CG, Angers S, Harris TJ. Mol. Biol. Cell 2015 Feb;26(4):711-25
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2014

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Coordinating the cytoskeleton and endocytosis for regulated plasma membrane growth in the early Drosophila embryo.Lee DM, Harris TJ. Bioarchitecture 2014 Mar-Apr;4(2):68-74
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Peptide binding properties of the three PDZ domains of Bazooka (Drosophila Par-3).Yu CG, Tonikian R, Felsensteiner C, Jhingree JR, Desveaux D, Sidhu SS, Harris TJ. PLoS ONE 2014;9(1):e86412
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2013

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Adherens junction distribution mechanisms during cell-cell contact elongation in Drosophila.Goldenberg G, Harris TJ. PLoS ONE 2013;8(11):e79613
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Bazooka inhibits aPKC to limit antagonism of actomyosin networks during amnioserosa apical constriction.David DJ, Wang Q, Feng JJ, Harris TJ. Development 2013 Dec;140(23):4719-29
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An Arf-GEF regulates antagonism between endocytosis and the cytoskeleton for Drosophila blastoderm development.Lee DM, Harris TJ. Curr. Biol. 2013 Nov;23(21):2110-20
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2012

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Displacement of basolateral Bazooka/PAR-3 by regulated transport and dispersion during epithelial polarization in Drosophila.McKinley RF, Harris TJ. Mol. Biol. Cell 2012 Nov;23(22):4465-71
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Interactions between the PDZ domains of Bazooka (Par-3) and phosphatidic acid: in vitro characterization and role in epithelial development.Yu CG, Harris TJ. Mol. Biol. Cell 2012 Sep;23(18):3743-53
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An introduction to adherens junctions: from molecular mechanisms to tissue development and disease.Harris TJ. Subcell. Biochem. 2012;60:1-5
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Assembly of Bazooka polarity landmarks through a multifaceted membrane-association mechanism.McKinley RF, Yu CG, Harris TJ. J. Cell. Sci. 2012 Mar;125(Pt 5):1177-90
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Adherens junction assembly and function in the Drosophila embryo.Harris TJ. Int Rev Cell Mol Biol 2012;293:45-83
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Live imaging of Drosophila embryos: quantifying protein numbers and dynamics at subcellular locations.David DJ, McGill MA, McKinley RF, Harris TJ. Methods Mol. Biol. 2012;839:1-17
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2010

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Adherens junctions: from molecules to morphogenesis.Harris TJ, Tepass U. Nat. Rev. Mol. Cell Biol. 2010 Jul;11(7):502-14
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The PAR complex regulates pulsed actomyosin contractions during amnioserosa apical constriction in Drosophila.David DJ, Tishkina A, Harris TJ. Development 2010 May;137(10):1645-55
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A modifier screen for Bazooka/PAR-3 interacting genes in the Drosophila embryo epithelium.Shao W, Wu J, Chen J, Lee DM, Tishkina A, Harris TJ. PLoS ONE 2010;5(4):e9938
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2009

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How the cytoskeleton helps build the embryonic body plan: models of morphogenesis from Drosophila.Harris TJ, Sawyer JK, Peifer M. Curr. Top. Dev. Biol. 2009;89:55-85
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Independent cadherin-catenin and Bazooka clusters interact to assemble adherens junctions.McGill MA, McKinley RF, Harris TJ. J. Cell Biol. 2009 Jun;185(5):787-96
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2008

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Control of cell flattening and junctional remodeling during squamous epithelial morphogenesis in Drosophila.Pope KL, Harris TJ. Development 2008 Jul;135(13):2227-38
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2007

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aPKC controls microtubule organization to balance adherens junction symmetry and planar polarity during development.Harris TJ, Peifer M. Dev. Cell 2007 May;12(5):727-38
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2005

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The positioning and segregation of apical cues during epithelial polarity establishment in Drosophila.Harris TJ, Peifer M. J. Cell Biol. 2005 Aug;170(5):813-23
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Decisions, decisions: beta-catenin chooses between adhesion and transcription.Harris TJ, Peifer M. Trends Cell Biol. 2005 May;15(5):234-7
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2004

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Regulation of cell-cell adhesion during Dictyostelium development.Siu CH, Harris TJ, Wang J, Wong E. Semin. Cell Dev. Biol. 2004 Dec;15(6):633-41
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Adherens junction-dependent and -independent steps in the establishment of epithelial cell polarity in Drosophila.Harris TJ, Peifer M. J. Cell Biol. 2004 Oct;167(1):135-47
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2003

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Cytoskeleton interactions involved in the assembly and function of glycoprotein-80 adhesion complexes in dictyostelium.Harris TJ, Ravandi A, Awrey DE, Siu CH. J. Biol. Chem. 2003 Jan;278(4):2614-23
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2002

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Reciprocal raft-receptor interactions and the assembly of adhesion complexes.Harris TJ, Siu CH. Bioessays 2002 Nov;24(11):996-1003
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2001

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Assembly of glycoprotein-80 adhesion complexes in Dictyostelium. Receptor compartmentalization and oligomerization in membrane rafts..Harris TJ, Ravandi A, Siu CH. J. Biol. Chem. 2001 Dec 28; 276(52):48764-74
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Involvement of a triton-insoluble floating fraction in Dictyostelium cell-cell adhesion..Harris TJ, Awrey DE, Cox BJ, Ravandi A, Tsang A, Siu CH. J. Biol. Chem. 2001 May 25; 276(21):18640-8
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