Course Description

This is an advanced laboratory course and assumes a strong background in molecular genetics and some prior laboratory experience. Students will develop the laboratory and team-work skills needed to succeed in the workplace, particularly the multi-disciplinary environment that integrates modern biological approaches.

The course will focus on molecular plant biology from DNA to RNA to protein. Since CSB491H1 is a team-based research course students will have to conduct their experiments semi-independently. This course consists of 2 parts. In the first half students will learn a variety of techniques. Based on this knowledge students will develop a research project which they will conduct in the second half of the course.

Some of the techniques covered in the course will include genotyping of mutants and transgenic plants, gene expression analysis by RT and Q-RT-PCR, protein expression by Western Blotting, and fluorescent microscopy. Furthermore, students will learn to apply basic genetic principles for mutant characterization. The goal of the course is to gain an understanding how to plan and conduct a research project as a team. l

The course also includes a tutorial session, where the different concepts relevant to the course will be discussed.


CSB330H1 / CSB350H1 with a minimum grade of 77% and approval of the instructor

Recommended Preparation

BIO251H1 or higher level plant biology course
BCH311H1 / CSB349H1 / MGY311Y1 taken concurrently

Not Offered

CSB491H1 is not offered in 2022-23.

Ancillary Fees

This course has a lab fee of $50.
Lab coat and safety glasses are required and the approximate cost is $25. Students are responsible for purchasing these items.

Balloted Course

This is a balloted course. You must apply to the Department of Cell and Systems Biology for admission. Please go to Undergraduate Forms for a ballot form.


Prof. D. Christendat
Prof. E. Nambara

Course Coordinator

Prof. W. Moeder

Contact Hours

48P, 12T

Required Text(s)/Readings

No required textbook.

Evaluation (Subject to change)

Four quizzes (10%)
Two lab reports (50%)
Two presentations (20%)
Lab performance (20%)

Last updated on May 31st, 2022