The Pseudomonas syringae Type III Effector HopF2 Suppresses Arabidopsis Stomatal Immunity

Hurley B, Lee D, Mott A, Wilton M, Liu J, Liu YC, Angers S, Coaker G, Guttman DS, Desveaux D

PLoS ONE 2014;9(12):e114921

PMID: 25503437


Pseudomonas syringae subverts plant immune signalling through injection of type III secreted effectors (T3SE) into host cells. The T3SE HopF2 can disable Arabidopsis immunity through Its ADP-ribosyltransferase activity. Proteomic analysis of HopF2 interacting proteins identified a protein complex containing ATPases required for regulating stomatal aperture, suggesting HopF2 may manipulate stomatal immunity. Here we report HopF2 can inhibit stomatal immunity independent of its ADP-ribosyltransferase activity. Transgenic expression of HopF2 in Arabidopsis inhibits stomatal closing in response to P. syringae and increases the virulence of surface inoculated P. syringae. Further, transgenic expression of HopF2 inhibits flg22 induced reactive oxygen species production. Intriguingly, ADP-ribosyltransferase activity is dispensable for inhibiting stomatal immunity and flg22 induced reactive oxygen species. Together, this implies HopF2 may be a bifunctional T3SE with ADP-ribosyltransferase activity required for inhibiting apoplastic immunity and an independent function required to inhibit stomatal immunity.

Congratulations to Prof. Jennifer Mitchell for great public interest in her new Genes & Development paper

Congratulations to Prof. Jennifer Mitchell on her recent Genes & Development paper:

A Sox2 distal enhancer cluster regulates embryonic stem cell differentiation potential

Harry Y. Zhou, Yulia Katsman, Navroop K. Dhaliwal, Scott Davidson, Neil N. Macpherson, Moorthy Sakthidevi, Felicia Collura and Jennifer A. Mitchell

Genes & Dev. 2014. 28: 2699-2711


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