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MSc Exit Seminar- ZhenHuan Xu
September 19, 2019 @ 10:00 am - 11:00 am
The Investigation of ABA Transport in Arabidopsis thaliana and Brassica napus
Abscisic acid (ABA) is a phytohormone that plays a major role in plant responses to biotic and abiotic stresses. Increases in ABA levels have been reported in conditions such as drought, high salinity and low temperature. Although the movement of ABA within plants has been documented, its migration pattern is still debatable. Recent application of Positron Emission Tomography (PET) on plants using radioactive tracers has provided a potential new method of studying ABA migration within plants. In order to study the localization pattern of 3’-F-ABA in planta, I performed histochemical analyses of b-Glucuronidase (GUS) expression in GUS reporter lines, and report that the radioactive tracer, 3’-18F-ABA, has similar bioactivity to ABA. Liquid chromatography tandem-mass spectrometry (LC-MS/MS)-based phytochemical analyses of 3’-F-ABA- or ABA-treated Arabidopsis thaliana and Brassica napus plants also showed a bi-directional distribution towards the reproductive organs and to the roots, suggesting a phloem-mediated transport of ABA under non-stressed conditions.
Supervisor: Prof. Eiji Nambara