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CSB Departmental Seminar: Allosteric regulation of cell adhesion machinery under tension
October 19, 2019 @ 11:00 am - 12:00 pm
Ph.D. Proteomics Scientist Noboru Ishiyama
Black Diamond Therapeutics
“Allosteric regulation of cell adhesion machinery under tension”
Abstract:
Our study aims to understand how α-catenin functions as a mechanosensitive F-actin-binding protein to control the cadherin-actin linkage crucial for cadherin-mediated cell-cell adhesion. We determined crystal structures of the dimeric α-catenin actin-binding domain (ABD) with an enhanced F-actin binding mutation, and discovered that an N-terminal α1-helix plays a key role in the binding and bundling of F-actin. Remarkably, our biochemical and cell biology data unveiled the involvement of α1-helix in the mechanosensory function of α-catenin during dynamic adherens junction (AJ) remodelling in cellulo and in vivo. Based on these results, we propose a new model of force-dependent interactions between cadherin-catenin complexes and the actin cytoskeleton facilitated by α-catenin at AJs.
Friday, Oct 18th, 2019
11:00 a.m
Ramsay Wright, 432
Host: Ulrich Tepass: u.tepass@utoronto.ca