CSB Seminar Series – Shane Hutson, PhD – Vanderbilt University

CSB Departmental Seminar

Shane Hutson, PhD

Vanderbilt University

TITLE: Something’s wrong in the cellular neighborhood: the biophysics of how cells respond to laser-induced wounds

ABSTRACT: When an epithelial layer of cells is injured, surrounding cells respond in a distance-dependent manner to reseal the wound. So, how do the surrounding cells “know” that there is a wound nearby, i.e., that something is wrong in the cellular neighborhood? Across a wide range of organisms, the first response of epithelial cells to local wounds is a dramatic increase in cytosolic calcium. We have investigated this process in fruit flies using fast and reproducible laser wounds. Our results show that this increase occurs quickly – calcium floods into damaged cells within 15 milliseconds, moves into adjacent cells over ~20 s, and appears in a much larger set of surrounding cells via a delayed second expansion over 40-300 s – but calcium is nonetheless a reporter: cells must detect wounds even earlier. We will discuss how measurements of laser-tissue interactions can be combined with quantitative image analysis and the genetic tools available in fruit flies to tease apart wound detection in terms of its biophysical and biochemical mechanisms. We will discuss the experimental evidence and a corresponding computational model developed to match experimental observations, test the plausibility of hypothesized mechanisms, and make experimentally testable predictions. We will then discuss how these early signals relate to subsequent cell behaviors such as the formation of wound-induced syncytia and the re-establishment of epithelial tension. This work supported by NIH Grant 1R01GM130130.

HOST: Tony Harris

LOCATION: Cell and Systems Biology, 25 Harbord Street, Suite 432