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MSc Exit Seminar – Nina Kirischian (Guttman/Desveaux lab)
June 27, 2017 @ 2:00 pm - 2:30 pm
MSc Exit Seminar
Tuesday June 27th, 2:00 pm – Earth Sciences Centre, Rm. 3087
Nina Kirischian (Guttman/Desveaux lab)
“Identification of novel resistance specificity in Arabidopsis thaliana against Pseudomonas syringae strains”
Abstract
Pseudomonas syringae is a plant pathogen that infects a diverse set of hosts by injecting type III secreted effectors (T3SEs) into plant cells to cause disease. Plants like Arabidopsis thaliana have evolved resistance (R) proteins to recognize these effectors and trigger robust immunity. Co-evolutionary pressures have led to effector and R protein diversification as plants and pathogens struggle for survival. To date, there are >60 T3SE families in P. syringae and ~160 predicted R proteins in Arabidopsis, yet, less than a dozen R protein-effector pairs are characterized. To tackle this problem, we took 2 approaches. 1) We screened Arabidopsis ecotypes for resistance to PtoDC3000. We identified the Uod-1 ecotype and propose HopM1 as a potential T3SE triggering immunity. 2) We screened P. syringae strains for those capable of triggering immunity in the Arabidopsis Col-0 ecotype. We identified two strains with immune-eliciting effectors that have yet to be characterized.