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MSc Exit Seminar – James Colapinto (Berleth lab)
January 11, 2017 @ 10:10 am - 10:40 am
MSc Exit Seminar
Wednesday January 11th, 10:10 am – Earth Sciences Centre, Rm. 3087
James Colapinto (Berleth lab)
“ Cis-regulatory Elements Controlling Expression of the Auxin Response Factor MONOPTEROS and its Irrepressible Variant MPΔ”
Abstract
The AUXIN RESPONSE FACTORS (ARFs) mediate changes in gene expression that are associated with auxin response in plants. One of the best-characterized ARFs is ARF5/MONOPTEROS(MP), for which strong loss-of-function and gain-of-function associated defects have been reported. Recent evidence has indicated that auxin response may be driven in part by MP-mediated control of its own expression. Here it is shown that removal of individual ARF-binding auxin response elements (AuxREs) from the MP promoter results in differential expression in specific tissues, while creation of supernumerary AuxREs is associated with auxin response-related phenotypic changes. The irrepressible MP variant MPΔ was used to track potentially expression-stage specific regions of the MP promoter and to determine in which tissues MPΔ can exert its gain-of-function effects independent of the activity of other, closely related ARFs.