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MSc Exit Seminar – Mia Husić (Lovejoy lab)
September 9, 2016 @ 11:30 am - 12:00 pm
MSc Exit Seminar
Friday September 9th, 11:30 am – Ramsay Wright Building, Rm. 432
Mia Husić (Lovejoy lab)
“Binding, activation and cellular actions of teneurin C-terminal associated peptide (TCAP)-1 with its putative receptor, latrophilin-1 (ADGRL1), in immortalized cell lines“
Abstract
The teneurins are multifunctional type-II transmembrane proteins that bind the adhesion G-protein coupled receptor subfamily L/latrophilin (ADGRL) in the only intermolecular synaptic adhesion unit conserved between invertebrates and vertebrates. However, the specific interaction between teneurin and ADGRL is not well understood. The distal extracellular region of teneurin possesses a potentially cleavable bioactive peptide termed the ‘teneurin C-terminal associated peptide’ (TCAP). A synthetic version of TCAP-1 is highly active in cells, causing increased expression of cytoskeletal components and actin re-arrangement. Yet the mechanism by which TCAP-1 induces these effects remains unclear. HCT116 cells over-expressing ADGRL1 have increased uptake of TCAP-1 compared to wild-type cells. ADGRL1 expression causes increased adhesion between cells and reduces their expression of f-actin. TCAP-1 treatment induces f-actin polymerization and modulation of cellular morphology only in ADGRL1-expressing cells. These data are the first to establish TCAP-1 as an endogenous ADGRL1 ligand, and further elucidate its mode of action.
Ramsay Wright is a wheelchair accessible building.