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MSc Exit Seminar – Peilu Gan -Tuesday, May 29, 2018
May 29, 2018 @ 10:10 am - 11:00 am
MSc Exit Seminar
Tuesday, May 29, 2018 at 10:10pm, SW 403- University of Toronto at Scarborough
Peilu Gan (Hasenkampf Lab)
The Role of the Arabidopsis Hop2 Protein in Promoting Homologous Chromosome Interactions and Blocking Nonhomologous Interactions
Abstract:
The Homologous Pairing Protein 2 (Hop2) is important for its role in reciprocal genetic exchange in meiosis. It is thought to operate as a part of the double-strand break (DSB) repair pathway. Recent models give two potential roles for Hop2: it acts to promote interactions between homologous chromosomes, or it acts to block interactions between non-homologous chromosomes. The goal of my study was to see if the Hop2 protein acted to block non-homologous interactions by analyzing its role in haploid plants. Haploid hop2-1 mutants were analyzed by light and fluorescent microscopy and compared with haploid WT plants. Like WT haploids, hop2-1 mutants showed univalents in early meiosis. However, unlike WT haploid plants, hop2-1 haploid mutants showed large amounts of DNA fragmentation and chromosomal bridging in anaphase and metaphase for both Meiosis I and Meiosis II. This suggests that Hop2 acts to block non-homologous interactions.