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MSc Exit Seminar – Reem Merwass (AbouHaidar lab)

May 12, 2016 @ 1:10 pm - 2:10 pm

MSc Exit Seminar

Thursday May 12th, 1:10 pm – Earth Sciences Centre, Rm. 3087

Reem Merwass (AbouHaidar lab)

Requirement(s) for the Replication of Lucerne Transient Streak Virus Satellite RNA


The satellite RNA of Lucerne Transient Streak Virus (LTSV) is a 322-nucleotide, single-stranded circular RNA with a high degree of base-pairing (70%) that has a rod-like structure very similar to that of viroids. As it does not encode any translation products and cannot replicate independently of a helper virus, the satellite RNA is proposed to rely on viral-encoded proteins for the replication and cell-to-cell movement that facilitate its systemic infection in a host. To investigate the requirements for replication and cell-to-cell movement of the LTSV satellite RNA, transgenic plant systems were generated to express the viral RNA-dependent RNA polymerase. Results of infectivity assays of these transgenic lines demonstrated for the first time that the viral encoded RNA-dependent RNA polymerase is necessary and sufficient for the replication of LTSV satellite RNA, and no additional viral-encoded protein products for its cell-to cell or systemic transport are needed. Since some studies of cell-to-cell movement in plants indicate that plasmodesmata have a molecular size exclusion limit between 700 and 800 daltons, the LTSV satellite RNA (with a molecular size of approximately 109 kDa) may be able to interact with plasmodesmata in such a way as to allow for its movement without the assistance of other viral or host-encoded proteins.


May 12, 2016
1:10 pm - 2:10 pm
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