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MSc Exit Seminar – Shafinaz Eisha (McGowan Lab)

August 27, 2021 @ 12:00 pm - 12:30 pm

Effect of Maternal High Fat Diet and Milk-Derived Exosome Supplementation on DNA Methyltransferase 1 in the Brain


Maternal high fat diet (mHFD) exposure during perinatal life shapes offspring’s neurodevelopment trajectories. mHFD also influences the bioactive composition of maternal milk, including milk-derived exosomes (MDEs), nanovesicles that transport functional microRNAs (miRNA). miRNA-148/152 regulate DNA methyltransferase-1 (DNMT1), enzyme that catalyzes DNA methylation modifications, which in turn downregulates fat-mass and obesity-associated (Fto) gene via promoter hypermethylation. The main objectives of this thesis were: 1) examine the effects of mHFD exposure on DNMTs and Fto expressions in postnatal day 7 rats and 2) investigate MDE-supplementation effects on DNMTs and Fto expressions using a human microglia cell line. DNMT1 and Fto transcript abundances remained unchanged in the PVN and ventral hippocampus but decreased in the retroperitoneal fat of mHFD offspring. In microglia, MDE-supplementation resulted in increased DNMT1 expression only. These findings indicate that DNMT1 and Fto exhibit tissue-specific sensitivity to mHFD exposure in neonates, and that DNMT1 is altered by MDE-supplementation in microglia.


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Meeting ID: 894 4303 3929

Host: Patrick McGowan (patrick.mcgowan@utoronto.ca)



August 27, 2021
12:00 pm - 12:30 pm
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