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PhD Proposal Examination – Lida Langroudi (Mitchell lab)

January 19, 2017 @ 11:10 am - 12:10 pm

PhD Proposal Exam


Thursday January 19th, 11:10 am – Ramsay Wright Building, Rm. 432


Lida Langroudi (Mitchell lab)

Evaluating the role of Myrf in early development


Throughout development, the precise spatio-temporal regulation of transcription is achieved through stage specific transcription factors (TFs) in response to internal and external queues. Using embryonic stem cells (mESCs) as a model system to recapitulate early embryonic fate indicated a robust up regulation of Myelin Regulatory Factor (MYRF) upon pluripotency exit. MYRF is well known for transcriptional regulation of myelination genes in oligodendrocytes, allowing terminal differentiation and survival in the central nervous system. It has been determined that MYRF works cooperatively with SOX10 to activate myelination genes by targeting their regulatory sequences. Although MYRF has been reported embryonic lethal, the causality and time of death has not been determined. Among the pluripotency factors that maintain self-renewal of ESCs, SOX2 is an essential transcription factor in embryonic development and ectodermal lineage where aberrations have been linked to many developmental defects. Considering the expression and relative association of MYRF and SOX family, I hypothesize that MYRF plays an essential role in early embryonic development by working cooperatively with SOX2. My preliminary data indicate co-expression and localization of MYRF with SOX2 in ESCs and pre-implanted embryos. First of all, to explore the role of MYRF in early embryonic development, I propose a knockout approach to examine the time and cause of embryonic lethality. Secondly, to investigate the transcriptional regulatory role of MYRF in ESCs, I will seek nuclear complexes associated with MYRF with emphasis on SOX2. Finally, by gaining new insight to cooperative factors involved with MYRF, I will determine the relative association and function of theses novel interactors in embryonic development.

Ramsay Wright is a wheelchair accessible building.




January 19, 2017
11:10 am - 12:10 pm
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Ramsay Wright Building, Room 432
25 Harbord St.
Toronto, ON M5S 3G5 Canada