Eiji Nambara
Professor
Campus
St. George (downtown)
CSB Appointment
Full
Research Areas
Biotechnology, Chemical Biology, Genetics / Genomics, Metabolomics, Molecular Biology, Plant Biology, Systems Biology
Education
Ph.D. Nagoya University 1994
Primary Undergraduate Department
Cell & Systems Biology
Graduate Programs
Cell & Systems Biology
Developmental Biology
Research Description
Plant hormones are low-abundant compounds, which elicit numerous biological processes essential for plant growth and development. Recent progress in functional genomics reveals that elucidation of hormone action mechanism is the key to engineer plants with enhanced performance as well as to evaluating the short- and long-term effects of genetic modifications in plants. My laboratory will study how hormone levels are regulated. In particular, we will focus on elucidating the molecular mechanisms by which regulate abscisic acid metabolism and movement.
Contact Information
Office Phone: 416-978-4668
Office: ESC4024
Lab: ESC4026
Lab Phone: 416-978-1604
Email
Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Willcocks St.
Toronto, ON M5S 3B2
Canada
Recent News
Publications
2024
Calcium signaling triggers early high humidity responses in Arabidopsis thaliana
Hussain S, Suda H, Nguyen CH, Yan D, Toyota M, Yoshioka K, Nambara E
2024, Proceedings of the National Academy of Sciences, 10.1073/pnas.2416270121
2023
Multi‐omics atlas of combinatorial abiotic stress responses in wheat
Da Ros L, Bollina V, Soolanayakanahally R, Pahari S, Elferjani R, Kulkarni M, Vaid N, Risseuw E, Cram D, Pasha A, Esteban E, Konkin D, Provart N, Nambara E, Kagale S
2023, The Plant Journal, 10.1111/tpj.16332
2020
flasher, a novel mutation in a glucosinolate modifying enzyme, conditions changes in plant architecture and hormone homeostasis
Garrido AN, Supijono E, Boshara P, Douglas SJ, Stronghill PE, Li B, Nambara E, Kliebenstein DJ, Riggs CD
2020, The Plant Journal, 10.1111/tpj.14878
Hydrolysis of abscisic acid glucose ester occurs locally and quickly in response to dehydration
Nambara E, Brookbank BP, Hussain S
2020, Journal of Experimental Botany, 10.1093/jxb/eraa026
2018
Family Members and Their Individual Roles: An Arabidopsis Arogenate Dehydratase ADT2 and its Role in Seed Development
Xu Z, Nambara E
2018, Plant and Cell Physiology, 10.1093/pcp/pcy222
2014
The Functions of the Endosperm During Seed Germination
Yan D, Duermeyer L, Leoveanu C, Nambara E
2014, Plant & cell physiology, 24964910
Functional characterization of xanthoxin dehydrogenase in rice
Endo A, Nelson KM, Thoms K, Abrams SR, Nambara E, Sato Y
2014, Journal of plant physiology, 171, 1231-40, 25014258
Designed abscisic acid analogs as antagonists of PYL-PP2C receptor interactions
Takeuchi J, Okamoto M, Akiyama T, Muto T, Yajima S, Sue M, Seo M, Kanno Y, Kamo T, Endo A, Nambara E, Hirai N, Ohnishi T, Cutler SR, Todoroki Y
2014, Nature chemical biology, 10, 477-82, 24792952
Amplification of ABA biosynthesis and signaling through a positive feedback mechanism in seeds
Nonogaki M, Sall K, Nambara E, Nonogaki H
2014, The Plant journal : for cell and molecular biology, 78, 527-39, 24520869
2013
Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance
Okamoto M, Peterson FC, Defries A, Park SY, Endo A, Nambara E, Volkman BF, Cutler SR
2013, Proceedings of the National Academy of Sciences of the United States of America, 110, 12132-7, 23818638
Interplay between sucrose and folate modulates auxin signaling in Arabidopsis
Stokes ME, Chattopadhyay A, Wilkins O, Nambara E, Campbell MM
2013, Plant physiology, 162, 1552-65, 23690535
Combining association mapping and transcriptomics identify HD2B histone deacetylase as a genetic factor associated with seed dormancy in Arabidopsis thaliana
Yano R, Takebayashi Y, Nambara E, Kamiya Y, Seo M
2013, The Plant journal : for cell and molecular biology, 74, 815-28, 23464703
2012
Seed biology in the 21st century: perspectives and new directions
Nambara E, Nonogaki H
2012, Plant & cell physiology, 53, 1-4, 22241887
2011
Thermoinhibition uncovers a role for strigolactones in Arabidopsis seed germination
Toh S, Kamiya Y, Kawakami N, Nambara E, McCourt P, Tsuchiya Y
2012, Plant & cell physiology, 53, 107-17, 22173099
Tissue-specific transcriptome analysis reveals cell wall metabolism, flavonol biosynthesis and defense responses are activated in the endosperm of germinating Arabidopsis thaliana seeds
Endo A, Tatematsu K, Hanada K, Duermeyer L, Okamoto M, Yonekura-Sakakibara K, Saito K, Toyoda T, Kawakami N, Kamiya Y, Seki M, Nambara E
2012, Plant & cell physiology, 53, 16-27, 22147073
Opening a new era of ABA research
Nambara E, Kuchitsu K
2011, Journal of plant research, 124, 431-5, 21698351
ABA 9′-hydroxylation is catalyzed by CYP707A in Arabidopsis
Okamoto M, Kushiro T, Jikumaru Y, Abrams SR, Kamiya Y, Seki M, Nambara E
2011, Phytochemistry, 72, 717-22, 21414645
Functional analysis of abscisic acid 8′-hydroxylase
Endo A, Kimura M, Kawakami N, Nambara E
2011, Methods in molecular biology (Clifton, N.J.), 773, 135-47, 21898254
2010
Comprehensive hormone profiling in developing Arabidopsis seeds: examination of the site of ABA biosynthesis, ABA transport and hormone interactions
Kanno Y, Jikumaru Y, Hanada A, Nambara E, Abrams SR, Kamiya Y, Seo M
2010, Plant & cell physiology, 51, 1988-2001, 20959378
A small-molecule screen identifies new functions for the plant hormone strigolactone
Tsuchiya Y, Vidaurre D, Toh S, Hanada A, Nambara E, Kamiya Y, Yamaguchi S, McCourt P
2010, Nature chemical biology, 6, 741-9, 20818397
The lesion-mimic mutant cpr22 shows alterations in abscisic acid signaling and abscisic acid insensitivity in a salicylic acid-dependent manner
Mosher S, Moeder W, Nishimura N, Jikumaru Y, Joo SH, Urquhart W, Klessig DF, Kim SK, Nambara E, Yoshioka K
2010, Plant physiology, 152, 1901-13, 20164209
Stored and neosynthesized mRNA in Arabidopsis seeds: effects of cycloheximide and controlled deterioration treatment on the resumption of transcription during imbibition
Kimura M, Nambara E
2010, Plant molecular biology, 73, 119-29, 20099072
Genome-wide analysis of endogenous abscisic acid-mediated transcription in dry and imbibed seeds of Arabidopsis using tiling arrays
Okamoto M, Tatematsu K, Matsui A, Morosawa T, Ishida J, Tanaka M, Endo TA, Mochizuki Y, Toyoda T, Kamiya Y, Shinozaki K, Nambara E, Seki M
2010, The Plant journal : for cell and molecular biology, 62, 39-51, 20088898
2009
Nitrate responses of Arabidopsis cho1 mutants: obvious only when excess nitrate is supplied
Nambara E, Yamagishi K, Tatematsu K, Nakabayashi K, Kamiya Y
2009, Plant signaling & behavior, 4, 1166-8, 20514237
Temporal expression patterns of hormone metabolism genes during imbibition of Arabidopsis thaliana seeds: a comparative study on dormant and non-dormant accessions
Preston J, Tatematsu K, Kanno Y, Hobo T, Kimura M, Jikumaru Y, Yano R, Kamiya Y, Nambara E
2009, Plant & cell physiology, 50, 1786-800, 19713425
CHOTTO1, a putative double APETALA2 repeat transcription factor, is involved in abscisic acid-mediated repression of gibberellin biosynthesis during seed germination in Arabidopsis
Yano R, Kanno Y, Jikumaru Y, Nakabayashi K, Kamiya Y, Nambara E
2009, Plant physiology, 151, 641-54, 19648230
Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry
Okamoto M, Hanada A, Kamiya Y, Yamaguchi S, Nambara E
2009, Methods in molecular biology (Clifton, N.J.), 495, 53-60, 19085147
2008
CHOTTO1, a double AP2 domain protein of Arabidopsis thaliana, regulates germination and seedling growth under excess supply of glucose and nitrate
Yamagishi K, Tatematsu K, Yano R, Preston J, Kitamura S, Takahashi H, McCourt P, Kamiya Y, Nambara E
2009, Plant & cell physiology, 50, 330-40, 19109301
The Arabidopsis abscisic acid catabolic gene CYP707A2 plays a key role in nitrate control of seed dormancy
Matakiadis T, Alboresi A, Jikumaru Y, Tatematsu K, Pichon O, Renou JP, Kamiya Y, Nambara E, Truong HN
2009, Plant physiology, 149, 949-60, 19074630
Vascular system is a node of systemic stress responses: Competence of the cell to synthesize abscisic acid and its responsiveness to external cues
Endo A, Koshiba T, Kamiya Y, Nambara E
2008, Plant signaling & behavior, 3, 1138-40, 19704460
High humidity induces abscisic acid 8′-hydroxylase in stomata and vasculature to regulate local and systemic abscisic acid responses in Arabidopsis
Okamoto M, Tanaka Y, Abrams SR, Kamiya Y, Seki M, Nambara E
2009, Plant physiology, 149, 825-34, 19036833
Interaction of light and hormone signals in germinating seeds
Seo M, Nambara E, Choi G, Yamaguchi S
2009, Plant molecular biology, 69, 463-72, 19031046
The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity
Piskurewicz U, Jikumaru Y, Kinoshita N, Nambara E, Kamiya Y, Lopez-Molina L
2008, The Plant cell, 20, 2729-45, 18941053
The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access
Goda H, Sasaki E, Akiyama K, Maruyama-Nakashita A, Nakabayashi K, Li W, Ogawa M, Yamauchi Y, Preston J, Aoki K, Kiba T, Takatsuto S, Fujioka S, Asami T, Nakano T, Kato H, Mizuno T, Sakakibara H, Yamaguchi S, Nambara E, Kamiya Y, Takahashi H, Hirai MY, Sakurai T, Shinozaki K, Saito K, Yoshida S, Shimada Y
2008, The Plant journal : for cell and molecular biology, 55, 526-42, 18419781
Arabidopsis transcriptome analysis under drought, cold, high-salinity and ABA treatment conditions using a tiling array
Matsui A, Ishida J, Morosawa T, Mochizuki Y, Kaminuma E, Endo TA, Okamoto M, Nambara E, Nakajima M, Kawashima M, Satou M, Kim JM, Kobayashi N, Toyoda T, Shinozaki K, Seki M
2008, Plant & cell physiology, 49, 1135-49, 18625610
Co-regulation of ribosomal protein genes as an indicator of growth status: Comparative transcriptome analysis on axillary shoots and seeds in Arabidopsis
Tatematsu K, Kamiya Y, Nambara E
2008, Plant signaling & behavior, 3, 450-2, 19704481
Drought induction of Arabidopsis 9-cis-epoxycarotenoid dioxygenase occurs in vascular parenchyma cells
Endo A, Sawada Y, Takahashi H, Okamoto M, Ikegami K, Koiwai H, Seo M, Toyomasu T, Mitsuhashi W, Shinozaki K, Nakazono M, Kamiya Y, Koshiba T, Nambara E
2008, Plant physiology, 147, 1984-93, 18550687
Phytochrome- and gibberellin-mediated regulation of abscisic acid metabolism during germination of photoblastic lettuce seeds
Sawada Y, Aoki M, Nakaminami K, Mitsuhashi W, Tatematsu K, Kushiro T, Koshiba T, Kamiya Y, Inoue Y, Nambara E, Toyomasu T
2008, Plant physiology, 146, 1386-96, 18184730
2007
High temperature-induced abscisic acid biosynthesis and its role in the inhibition of gibberellin action in Arabidopsis seeds
Toh S, Imamura A, Watanabe A, Nakabayashi K, Okamoto M, Jikumaru Y, Hanada A, Aso Y, Ishiyama K, Tamura N, Iuchi S, Kobayashi M, Yamaguchi S, Kamiya Y, Nambara E, Kawakami N
2008, Plant physiology, 146, 1368-85, 18162586
Transient expression of AtNCED3 and AAO3 genes in guard cells causes stomatal closure in Vicia faba
Melhorn V, Matsumi K, Koiwai H, Ikegami K, Okamoto M, Nambara E, Bittner F, Koshiba T
2008, Journal of plant research, 121, 125-31, 18060348
Transcription factor AtTCP14 regulates embryonic growth potential during seed germination in Arabidopsis thaliana
Tatematsu K, Nakabayashi K, Kamiya Y, Nambara E
2008, The Plant journal : for cell and molecular biology, 53, 42-52, 17953649
Global analysis of della direct targets in early gibberellin signaling in Arabidopsis
Zentella R, Zhang ZL, Park M, Thomas SG, Endo A, Murase K, Fleet CM, Jikumaru Y, Nambara E, Kamiya Y, Sun TP
2007, The Plant cell, 19, 3037-57, 17933900
Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8′-hydroxylase in rice
Saika H, Okamoto M, Miyoshi K, Kushiro T, Shinoda S, Jikumaru Y, Fujimoto M, Arikawa T, Takahashi H, Ando M, Arimura S, Miyao A, Hirochika H, Kamiya Y, Tsutsumi N, Nambara E, Nakazono M
2007, Plant & cell physiology, 48, 287-98, 17205969
2006
Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism
Seo M, Hanada A, Kuwahara A, Endo A, Okamoto M, Yamauchi Y, North H, Marion-Poll A, Sun TP, Koshiba T, Kamiya Y, Yamaguchi S, Nambara E
2006, The Plant journal : for cell and molecular biology, 48, 354-66, 17010113
A quick release mechanism for abscisic acid
Schroeder JI, Nambara E
2006, Cell, 126, 1023-5, 16990126
A plant growth retardant, uniconazole, is a potent inhibitor of ABA catabolism in Arabidopsis
Saito S, Okamoto M, Shinoda S, Kushiro T, Koshiba T, Kamiya Y, Hirai N, Todoroki Y, Sakata K, Nambara E, Mizutani M
2006, Bioscience, biotechnology, and biochemistry, 70, 1731-9, 16819156
Field studies on the regulation of abscisic acid content and germinability during grain development of barley: molecular and chemical analysis of pre-harvest sprouting
Chono M, Honda I, Shinoda S, Kushiro T, Kamiya Y, Nambara E, Kawakami N, Kaneko S, Watanabe Y
2006, Journal of experimental botany, 57, 2421-34, 16798848
CYP707A3, a major ABA 8′-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana
Umezawa T, Okamoto M, Kushiro T, Nambara E, Oono Y, Seki M, Kobayashi M, Koshiba T, Kamiya Y, Shinozaki K
2006, The Plant journal : for cell and molecular biology, 46, 171-82, 16623881
CYP707A1 and CYP707A2, which encode abscisic acid 8′-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis
Okamoto M, Kuwahara A, Seo M, Kushiro T, Asami T, Hirai N, Kamiya Y, Koshiba T, Nambara E
2006, Plant physiology, 141, 97-107, 16543410
Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy
Lefebvre V, North H, Frey A, Sotta B, Seo M, Okamoto M, Nambara E, Marion-Poll A
2006, The Plant journal : for cell and molecular biology, 45, 309-19, 16412079
2005
Identification of cis-elements that regulate gene expression during initiation of axillary bud outgrowth in Arabidopsis
Tatematsu K, Ward S, Leyser O, Kamiya Y, Nambara E
2005, Plant physiology, 138, 757-66, 15908603
Genome-wide profiling of stored mRNA in Arabidopsis thaliana seed germination: epigenetic and genetic regulation of transcription in seed
Nakabayashi K, Okamoto M, Koshiba T, Kamiya Y, Nambara E
2005, The Plant journal : for cell and molecular biology, 41, 697-709, 15703057
Abscisic acid biosynthesis and catabolism
Nambara E, Marion-Poll A
2005, Annual review of plant biology, 56, 165-85, 15862093
2004
Comparative studies on the Arabidopsis aldehyde oxidase (AAO) gene family revealed a major role of AAO3 in ABA biosynthesis in seeds
Seo M, Aoki H, Koiwai H, Kamiya Y, Nambara E, Koshiba T
2004, Plant & cell physiology, 45, 1694-703, 15574845
The Arabidopsis cytochrome P450 CYP707A encodes ABA 8′-hydroxylases: key enzymes in ABA catabolism
Kushiro T, Okamoto M, Nakabayashi K, Yamagishi K, Kitamura S, Asami T, Hirai N, Koshiba T, Kamiya Y, Nambara E
2004, The EMBO journal, 23, 1647-56, 15044947
The FUS3 transcription factor functions through the epidermal regulator TTG1 during embryogenesis in Arabidopsis
Tsuchiya Y, Nambara E, Naito S, McCourt P
2004, The Plant journal : for cell and molecular biology, 37, 73-81, 14675433
2003
ABA action and interactions in seeds
Nambara E, Marion-Poll A
2003, Trends in plant science, 8, 213-7, 12758038
Hormone evolution: The key to signalling
Kushiro T, Nambara E, McCourt P
2003, Nature, 422, 122, 12634761
2002
A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions
Cheng WH, Endo A, Zhou L, Penney J, Chen HC, Arroyo A, Leon P, Nambara E, Asami T, Seo M, Koshiba T, Sheen J
2002, The Plant cell, 14, 2723-43, 12417697
Storage proteins
Fujiwara T, Nambara E, Yamagishi K, Goto DB, Naito S
2002, The Arabidopsis book / American Society of Plant Biologists, 1, e0020, 22303197