Emma Master
Professor
Campus
St. George (downtown)
CSB Appointment
Cross
Research Areas
Biotechnology, Chemical Biology, Genetics / Genomics, Microbiology, Molecular Biology, Proteomics
Education
2002: Ph.D. Environmental Biochemistry, University of British Columbia, Vancouver, B.C., Canada
1995: B.Sc (Honours) Microbiology & Immunology, McGill University, Montreal, Canada
Primary Undergraduate Department
Chemical Engineering and Applied Chemistry
Graduate Programs
Chemical Engineering and Applied Chemistry, Cells and Systems Biology
Academic or Administrative Appointments
Associate Chair Research (CHE), Associate Director of BioZone
Research Description
Our goal is to discover, design and produce biocatalysts that tailor the chemical and physical functionality of major renewable bioresources (e.g., cellulose, hemicellulose, lignin, chitin) for their broader use in value-added materials.
Our focus on biocatalysts that upgrade, rather than degrade, major bioresources provide biomanufacturing technologies that generate bio-based materials with high atom efficiency and potential to reduce reliance on petrochemicals.
Our approach benefits from three key advantages of biocatalyts: 1) catalytic specificity that allows predictable modification of complex substrates, 2) mild and waster-based reaction requirements that retain beneficial structural properties of the starting material, and 3) exquisite ability to create new biocatalyst designs through protein modelling and engineering, expanding their potential in synthetic applications.
Contact Information
Office Phone: 416-946-7861
Office: WB420C
Lab: BioZone Centre for Applied Bioscience and Bioengineering
Email
Mailing Address
Department of Chemical Engineering & Applied Chemistry
University of Toronto
200 College St.
Toronto, ON M5S 3E5
Canada
Publications
2023
Xylan glucuronic acid side chains fix suberin‐like aliphatic compounds to wood cell walls
Derba‐Maceluch M, Mitra M, Hedenström M, Liu X, Gandla ML, Barbut FR, Abreu IN, Donev EN, Urbancsok J, Moritz T, Jönsson LJ, Tsang A, Powlowski J, Master ER, Mellerowicz EJ
2023, New Phytologist, 10.1111/nph.18712
Loosenin-Like Proteins from Phanerochaete carnosa Impact Both Cellulose and Chitin Fiber Networks
Monschein M, Ioannou E, Koitto T, Al Amin LAKM, Varis JJ, Wagner ER, Mikkonen KS, Cosgrove DJ, Master ER, Nygård Y
2023, Applied and Environmental Microbiology, 10.1128/aem.01863-22
2022
Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93.
Zhao H, Karppi J, Nguyen TTM, Bellemare A, Tsang A, Master E, Tenkanen M
2022, Biotechnology for biofuels and bioproducts, 15, 135, 36476312
From acetone fractionation to lignin-based phenolic and polyurethane resins
Arefmanesh M, Nikafshar S, Master ER, Nejad M
2022, Industrial Crops and Products, 10.1016/j.indcrop.2022.114604
Proteome of the Wood Decay Fungus Fomitopsis pinicola Is Altered by Substrate
Sabat G, Ahrendt S, Wu B, Gaskell J, Held BW, Toapanta C, Vuong TV, Lipzen A, Zhang J, Schilling JS, Master E, Grigoriev IV, Blanchette RA, Hibbett DS, Bhatnagar J, Cullen D, Rokas A
2022, Microbiology Resource Announcements, 10.1128/mra.00586-22
Enzymatic upgrading of heteroxylans for added-value chemicals and polymers
Vuong TV, Master ER
2022, Current Opinion in Biotechnology, 10.1016/j.copbio.2021.07.001
Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans
Penttinen L, Kouhi V, Fauré R, Skarina T, Stogios P, Master E, Jurak E
2022, Molecules, 10.3390/molecules27092655
PACER: a novel 3D plant cell wall model for the analysis of non-catalytic and enzymatic responses
Monschein M, Jurak E, Paasela T, Koitto T, Lambauer V, Pavicic M, Enjalbert T, Dumon C, Master ER
2022, Biotechnology for Biofuels and Bioproducts, 10.1186/s13068-022-02128-8
Enzymatic synthesis of kraft lignin-acrylate copolymers using an alkaline tolerant laccase
Arefmanesh M, Vuong TV, Nikafshar S, Wallmo H, Nejad M, Master ER
2022, Applied Microbiology and Biotechnology, 10.1007/s00253-022-11916-z
An SCPPPQ1/LAM332 protein complex enhances the adhesion and migration of oral epithelial cells: Implications for dentogingival regeneration
Nouri S, Holcroft J, Caruso L, Vuong TV, Simmons CA, Master ER, Ganss B
2022, Acta Biomaterialia, 10.1016/j.actbio.2022.05.035
Chitin-Active Lytic Polysaccharide Monooxygenases Are Rare in Cellulomonas Species
Li J, Goddard-Borger ED, Raji O, Saxena H, Solhi L, Mathieu Y, Master ER, Wakarchuk WW, Brumer H, Kelly RM
2022, Applied and Environmental Microbiology, 10.1128/aem.00968-22
2021
The coordinated action of glucuronoyl esterase and α‐glucuronidase promotes the disassembly of lignin–carbohydrate complexes
Raji O, Arnling Bååth J, Vuong TV, Larsbrink J, Olsson L, Master ER
2021, FEBS Letters, 10.1002/1873-3468.14019
Could open science stimulate industry partnerships in chemical engineering university research?
Hart K, An S, Edwards AM, Mahadevan R, Master ER, Edwards EA
2021, The Canadian Journal of Chemical Engineering, 10.1002/cjce.24077
Structural characterization of the family GH115 α-glucuronidase from Amphibacillus xylanus yields insight into its coordinated action with α-arabinofuranosidases
Yan R, Wang W, Vuong TV, Xiu Y, Skarina T, Di Leo R, Gatenholm P, Toriz G, Tenkanen M, Stogios PJ, Master ER
2021, New Biotechnology, 10.1016/j.nbt.2021.01.005
Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs
Haddad Momeni M, Fredslund F, Bissaro B, Raji O, Vuong TV, Meier S, Nielsen TS, Lombard V, Guigliarelli B, Biaso F, Haon M, Grisel S, Henrissat B, Welner DH, Master ER, Berrin J, Abou Hachem M
2021, Nature Communications, 10.1038/s41467-021-22372-0
Polysaccharide utilization loci-driven enzyme discovery reveals BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylans
Hameleers L, Penttinen L, Ikonen M, Jaillot L, Fauré R, Terrapon N, Deuss PJ, Hakulinen N, Master ER, Jurak E
2021, Biotechnology for Biofuels, 10.1186/s13068-021-01976-0
The Comparative Abilities of a Small Laccase and a Dye-Decoloring Peroxidase From the Same Bacterium to Transform Natural and Technical Lignins
Vuong TV, Singh R, Eltis LD, Master ER
2021, Frontiers in Microbiology, 10.3389/fmicb.2021.723524
2020
Enzymatic production of 4–methyl d-glucaric acid from hardwood xylan.
Vuong TV, Master ER
2020, Biotechnology for biofuels, 13, 51, 32190116
Atypical lignification in eastern leatherwood (Dirca palustris)
Mottiar Y, Gierlinger N, Jeremic D, Master ER, Mansfield SD
2020, New Phytologist, 10.1111/nph.16394
Quantitative Comparison of Pyranose Dehydrogenase Action on Diverse Xylooligosaccharides
Karppi J, Zhao H, Chong S, Koistinen AE, Tenkanen M, Master E
2020, Frontiers in Chemistry, 10.3389/fchem.2020.00011
2019
Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades
Aumala V, Mollerup F, Jurak E, Blume F, Karppi J, Koistinen AE, Schuiten E, Voß M, Bornscheuer U, Deska J, Master ER
2019, ChemSusChem, 10.1002/cssc.201802580
RETRACTED ARTICLE: Evolution of substrate-specific gene expression and RNA editing in brown rot wood-decaying fungi
Wu B, Gaskell J, Zhang J, Toapanta C, Ahrendt S, Grigoriev IV, Blanchette RA, Schilling JS, Master E, Cullen D, Hibbett DS
2019, The ISME Journal, 10.1038/s41396-019-0359-2
Kinetics and regioselectivity of three GH62 α-L-arabinofuranosidases from plant pathogenic fungi
Sarch C, Suzuki H, Master ER, Wang W
2019, Biochimica et Biophysica Acta (BBA) - General Subjects, 10.1016/j.bbagen.2019.03.020
A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family
Berrin J, Mollerup F, Aumala V, Parikka K, Mathieu Y, Brumer H, Tenkanen M, Master E
2019, PLOS ONE, 10.1371/journal.pone.0216546
2018
Chemo-enzymatic Synthesis of Clickable Xylo-oligosaccharide Monomers from Hardwood 4-O-Methylglucuronoxylan
MacCormick B, Vuong TV, Master ER
2018, Biomacromolecules, 10.1021/acs.biomac.7b01642
Direct analysis by time-of-flight secondary ion mass spectrometry reveals action of bacterial laccase-mediator systems on both hardwood and softwood samples
Goacher RE, Braham EJ, Michienzi CL, Flick RM, Yakunin AF, Master ER
2018, Physiologia Plantarum, 10.1111/ppl.12688
Pichia pastoris is a Suitable Host for the Heterologous Expression of Predicted Class I and Class II Hydrophobins for Discovery, Study, and Application in Biotechnology
Gandier J, Master E
2018, Microorganisms, 10.3390/microorganisms6010003
A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans
Razeq FM, Jurak E, Stogios PJ, Yan R, Tenkanen M, Kabel MA, Wang W, Master ER
2018, Biotechnology for Biofuels, 10.1186/s13068-018-1074-3
Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot Fungus Fomitopsis pinicola
Wu B, Gaskell J, Held BW, Toapanta C, Vuong T, Ahrendt S, Lipzen A, Zhang J, Schilling JS, Master E, Grigoriev IV, Blanchette RA, Cullen D, Hibbett DS, Kivisaar M
2018, Applied and Environmental Microbiology, 10.1128/AEM.00991-18
Analysis of the substrate specificity of α-L-arabinofuranosidases by DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis
Maurício da Fonseca MJ, Jurak E, Kataja K, Master ER, Berrin J, Stals I, Desmet T, Van Landschoot A, Briers Y
2018, Applied Microbiology and Biotechnology, 10.1007/s00253-018-9389-3
Dynamics of the Phanerochaete carnosa transcriptome during growth on aspen and spruce
Jurak E, Suzuki H, van Erven G, Gandier JA, Wong P, Chan K, Ho CY, Gong Y, Tillier E, Rosso M, Kabel MA, Miyauchi S, Master ER
2018, BMC Genomics, 10.1186/s12864-018-5210-z
2017
Microplate-Based Detection of Lytic Polysaccharide Monooxygenase Activity by Fluorescence-Labeling of Insoluble Oxidized Products
Vuong TV, Liu B, Sandgren M, Master ER
2017, Biomacromolecules, 10.1021/acs.biomac.6b01790
Transgenic expression of fungal accessory hemicellulases in Arabidopsis thaliana triggers transcriptional patterns related to biotic stress and defense response
Zabotina OA, Tsai AY, Chan K, Ho C, Canam T, Capron R, Master ER, Bräutigam K
2017, PLOS ONE, 10.1371/journal.pone.0173094
Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision
Gandier J, Langelaan DN, Won A, O’Donnell K, Grondin JL, Spencer HL, Wong P, Tillier E, Yip C, Smith SP, Master ER
2017, Scientific Reports, 10.1038/srep45863
Enzymatically Debranched Xylans in Graft Copolymerization
Littunen K, Mai-Gisondi G, Seppälä J, Master ER
2017, Biomacromolecules, 10.1021/acs.biomac.7b00229
Functional comparison of versatile carbohydrate esterases from families CE1, CE6 and CE16 on acetyl-4-O-methylglucuronoxylan and acetyl-galactoglucomannan
Mai-Gisondi G, Maaheimo H, Chong S, Hinz S, Tenkanen M, Master E
2017, Biochimica et Biophysica Acta (BBA) - General Subjects, 10.1016/j.bbagen.2017.06.002
Draft genome sequence of a monokaryotic model brown-rot fungus Postia (Rhodonia) placenta SB12
Gaskell J, Kersten P, Larrondo LF, Canessa P, Martinez D, Hibbett D, Schmoll M, Kubicek CP, Martinez AT, Yadav J, Master E, Magnuson JK, Yaver D, Berka R, Lail K, Chen C, LaButti K, Nolan M, Lipzen A, Aerts A, Riley R, Barry K, Henrissat B, Blanchette R, Grigoriev IV, Cullen D
2017, Genomics Data, 10.1016/j.gdata.2017.08.003
Action of a GH115 α-glucuronidase from Amphibacillus xylanus at alkaline condition promotes release of 4- O -methylglucopyranosyluronic acid from glucuronoxylan and arabinoglucuronoxylan
Yan R, Vuong TV, Wang W, Master ER
2017, Enzyme and Microbial Technology, 10.1016/j.enzmictec.2017.05.004
A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family
Andberg M, Mollerup F, Parikka K, Koutaniemi S, Boer H, Juvonen M, Master E, Tenkanen M, Kruus K, Cullen D
2017, Applied and Environmental Microbiology, 10.1128/AEM.01383-17
Replacing 100% of phenol in phenolic adhesive formulations with lignin
Kalami S, Arefmanesh M, Master E, Nejad M
2017, Journal of Applied Polymer Science, 10.1002/app.45124
Comparative Metagenomics of Cellulose- and Poplar Hydrolysate-Degrading Microcosms from Gut Microflora of the Canadian Beaver (Castor canadensis) and North American Moose (Alces americanus) after Long-Term Enrichment
Wong MT, Wang W, Couturier M, Razeq FM, Lombard V, Lapebie P, Edwards EA, Terrapon N, Henrissat B, Master ER
2017, Frontiers in Microbiology, 10.3389/fmicb.2017.02504
Constructing arabinofuranosidases for dual arabinoxylan debranching activity
Wang W, Andric N, Sarch C, Silva BT, Tenkanen M, Master ER
2017, Biotechnology and Bioengineering, 10.1002/bit.26445
2014
Expression of a fungal glucuronoyl esterase in Populus: Effects on wood properties and saccharification efficiency
Latha Gandla M, Derba-Maceluch M, Liu X, Gerber L, Master ER, Mellerowicz EJ, Jönsson LJ
2014, Phytochemistry, 24997793
Sequence diversity and gene expression analyses of expansin-related proteins in the white-rot basidiomycete, Phanerochaete carnosa
Suzuki H, Vuong TV, Gong Y, Chan K, Ho CY, Master ER, Kondo A
2014, Fungal genetics and biology : FG & B, 24880035
Elucidation of the Molecular Basis for Arabinoxylan-Debranching Activity of a Thermostable Family GH62 α-l-Arabinofuranosidase from Streptomyces thermoviolaceus
Wang W, Mai-Gisondi G, Stogios PJ, Kaur A, Xu X, Cui H, Turunen O, Savchenko A, Master ER
2014, Applied and environmental microbiology, 80, 5317-29, 24951792
The impact of alkali pretreatment and post-pretreatment conditioning on the surface properties of rice straw affecting cellulose accessibility to cellulases
Karuna N, Zhang L, Walton JH, Couturier M, Oztop MH, Master ER, McCarthy MJ, Jeoh T
2014, Bioresource technology, 167, 232-40, 24983695
Fusion of a xylan-binding module to gluco-oligosaccharide oxidase increases activity and promotes stable immobilization
Vuong TV, Master ER
2014, PloS one, 9, e95170, 24736604
Advancing lignocellulose bioconversion through direct assessment of enzyme action on insoluble substrates
Goacher RE, Selig MJ, Master ER
2014, Current opinion in biotechnology, 27, 123-33, 24525082
2013
Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictum and variants with reduced substrate inhibition
Vuong TV, Vesterinen AH, Foumani M, Juvonen M, Seppälä J, Tenkanen M, Master ER
2013, Biotechnology for biofuels, 6, 148, 24119501
Towards practical time-of-flight secondary ion mass spectrometry lignocellulolytic enzyme assays
Goacher RE, Tsai AY, Master ER
2013, Biotechnology for biofuels, 6, 132, 24034438
Biochemical studies of the multicopper oxidase (small laccase) from Streptomyces coelicolor using bioactive phytochemicals and site-directed mutagenesis
Sherif M, Waung D, Korbeci B, Mavisakalyan V, Flick R, Brown G, Abou-Zaid M, Yakunin AF, Master ER
2013, Microbial biotechnology, 6, 588-97, 23815400
2012
Comparative genomics of the white-rot fungi, Phanerochaete carnosa and P. chrysosporium, to elucidate the genetic basis of the distinct wood types they colonize
Suzuki H, MacDonald J, Syed K, Salamov A, Hori C, Aerts A, Henrissat B, Wiebenga A, VanKuyk PA, Barry K, Lindquist E, LaButti K, Lapidus A, Lucas S, Coutinho P, Gong Y, Samejima M, Mahadevan R, Abou-Zaid M, de Vries RP, Igarashi K, Yadav JS, Grigoriev IV, Master ER
2012, BMC genomics, 13, 444, 22937793
Constitutive expression of a fungal glucuronoyl esterase in Arabidopsis reveals altered cell wall composition and structure
Tsai AY, Canam T, Gorzsás A, Mellerowicz EJ, Campbell MM, Master ER
2012, Plant biotechnology journal, 10, 1077-87, 22924998
Mode of coniferous wood decay by the white rot fungus Phanerochaete carnosa as elucidated by FTIR and ToF-SIMS
Mahajan S, Jeremic D, Goacher RE, Master ER
2012, Applied microbiology and biotechnology, 94, 1303-11, 22290642
Application of time-of-flight-secondary ion mass spectrometry for the detection of enzyme activity on solid wood substrates
Goacher RE, Edwards EA, Yakunin AF, Mims CA, Master ER
2012, Analytical chemistry, 84, 4443-51, 22507179
Expression and regulation of genes encoding lignocellulose-degrading activity in the genus Phanerochaete
MacDonald J, Suzuki H, Master ER
2012, Applied microbiology and biotechnology, 94, 339-51, 22391967
2011
Time-dependent profiles of transcripts encoding lignocellulose-modifying enzymes of the white rot fungus Phanerochaete carnosa grown on multiple wood substrates
Macdonald J, Master ER
2012, Applied and environmental microbiology, 78, 1596-600, 22210217
Altered substrate specificity of the gluco-oligosaccharide oxidase from Acremonium strictum
Foumani M, Vuong TV, Master ER
2011, Biotechnology and bioengineering, 108, 2261-9, 21455933
Transcriptomic responses of the softwood-degrading white-rot fungus Phanerochaete carnosa during growth on coniferous and deciduous wood
MacDonald J, Doering M, Canam T, Gong Y, Guttman DS, Campbell MM, Master ER
2011, Applied and environmental microbiology, 77, 3211-8, 21441342
2010
Expanding the library of secondary ions that distinguish lignin and polysaccharides in time-of-flight secondary ion mass spectrometry analysis of wood
Goacher RE, Jeremic D, Master ER
2011, Analytical chemistry, 83, 804-12, 21190327
Mining bacterial genomes for novel arylesterase activity
Wang L, Mavisakalyan V, Tillier ER, Clark GW, Savchenko AV, Yakunin AF, Master ER
2010, Microbial biotechnology, 3, 677-90, 21255363
Proteomic characterization of lignocellulose-degrading enzymes secreted by Phanerochaete carnosa grown on spruce and microcrystalline cellulose
Mahajan S, Master ER
2010, Applied microbiology and biotechnology, 86, 1903-14, 20306191
2009
KORRIGAN1 and its aspen homolog PttCel9A1 decrease cellulose crystallinity in Arabidopsis stems
Takahashi J, Rudsander UJ, Hedenström M, Banasiak A, Harholt J, Amelot N, Immerzeel P, Ryden P, Endo S, Ibatullin FM, Brumer H, del Campillo E, Master ER, Scheller HV, Sundberg B, Teeri TT, Mellerowicz EJ
2009, Plant & cell physiology, 50, 1099-115, 19398462
Substrate recognition and hydrolysis by a fungal xyloglucan-specific family 12 hydrolase
Powlowski J, Mahajan S, Schapira M, Master ER
2009, Carbohydrate research, 344, 1175-9, 19433323
2008
Biphenyl dioxygenase from an arctic isolate is not cold adapted
Master ER, Agar NY, Gómez-Gil L, Powlowski JB, Mohn WW, Eltis LD
2008, Applied and environmental microbiology, 74, 3908-11, 18424535
Comparative NMR analysis of cellooligosaccharide hydrolysis by GH9 bacterial and plant endo-1,4-beta-glucanases
Rudsander UJ, Sandstrom C, Piens K, Master ER, Wilson DB, Brumer Iii H, Kenne L, Teeri TT
2008, Biochemistry, 47, 5235-41, 18402467
A xyloglucan-specific family 12 glycosyl hydrolase from Aspergillus niger: recombinant expression, purification and characterization
Master ER, Zheng Y, Storms R, Tsang A, Powlowski J
2008, The Biochemical journal, 411, 161-70, 18072936
2005
Biphenyl uptake by psychrotolerant Pseudomonas sp. strain Cam-1 and mesophilic Burkholderia sp. strain LB400
Master ER, McKinlay JJ, Stewart GR, Mohn WW
2005, Canadian journal of microbiology, 51, 399-404, 16088335
2004
Recombinant expression and enzymatic characterization of PttCel9A, a KOR homologue from Populus tremula x tremuloides
Master ER, Rudsander UJ, Zhou W, Henriksson H, Divne C, Denman S, Wilson DB, Teeri TT
2004, Biochemistry, 43, 10080-9, 15287736
2003
N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A
Henriksson H, Denman SE, Campuzano ID, Ademark P, Master ER, Teeri TT, Brumer H
2003, The Biochemical journal, 375, 61-73, 12826015