Josh Milstein

Associate Professor



CSB Appointment


Research Areas

Bioinformatics / Computational Biology, Microbiology, Quantitative Biology /Modelling


Ph.D. University of Colorado, Boulder 2004

Primary Undergraduate Department

Chemical and Physical Sciences, UTM

Graduate Programs

Physics, Cell & Systems Biology

Research Description

We are interested in problems ranging from the molecular basis of cell signaling to the ecology and population dynamics of bacteria within biofilms and the microbiome.

Contact Information

Office Phone: 905-569-4598
Office: DV4053
Lab: DV5032

Mailing Address

Department of Cell & Systems Biology
University of Toronto
3359 Mississauga Road
Mississauga, ON L5L 1C6

Visit lab’s website



Single-molecule counting applied to the study of GPCR oligomerization

Milstein JN, Nino DF, Zhou X, Gradinaru CC
2022, Biophysical Journal, 10.1016/j.bpj.2022.07.034

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An Ultrastable and Dense Single‐Molecule Click Platform for Sensing Protein–Deoxyribonucleic Acid Interactions

Visser EWA, Miladinovic J, Milstein JN
2021, Small Methods, 10.1002/smtd.202001180

Estimating the dynamic range of quantitative single-molecule localization microscopy

Nino DF, Milstein JN
2021, Biophysical Journal, 10.1016/j.bpj.2021.08.024

Highly Potent Photoinactivation of Bacteria Using a Water-Soluble, Cell-Permeable, DNA-Binding Photosensitizer

Digby EM, Ma T, Zipfel WR, Milstein JN, Beharry AA
2021, ACS Infectious Diseases, 10.1021/acsinfecdis.1c00313

An expectation–maximization approach to quantifying protein stoichiometry with single-molecule imaging

Boonkird A, Nino DF, Milstein JN, Bateman A
2021, Bioinformatics Advances, 10.1093/bioadv/vbab032

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FOCAL3D: A 3-dimensional clustering package for single-molecule localization microscopy

Papin JA, Nino DF, Djayakarsana D, Milstein JN
2020, PLOS Computational Biology, 10.1371/journal.pcbi.1008479

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