Leslie T. Buck
Professor
Campus
St. George (downtown)
CSB Appointment
Full
Research Areas
Animal Biology, Cell Biology, Neurobiology, Physiology
Education
Ph.D. University of British Columbia 1993
B.Sc.. Brock University 1986
Primary Undergraduate Department
Cell & Systems Biology
Graduate Programs
Cell & Systems Biology
Collaborative Program in Neuroscience
Academic or Administrative Appointments
Associate Chair for Graduate Studies – July 1, 2009 to July 1, 2015
Research Description
We use a comparative neurobiological/physiological/molecular approach to understand how animals survive environmental extremes, in particular how painted turtles and goldfish survive without oxygen for weeks.
Contact Information
Office Phone: 416-978-3506
Office: RW 329B
Lab: RW328/RW329
Lab Phone: 416-946-7238
Email
Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Harbord St.
Toronto, ON M5S 3G5
Canada
Recent News
Publications
2021
GABA receptor inhibition and severe hypoxia induce a paroxysmal depolarization shift in goldfish neurons
Hossein-Javaheri N, Buck LT
2021, Journal of Neurophysiology, 10.1152/jn.00149.2020
Scavenging of reactive oxygen species mimics the anoxic response in goldfish pyramidal neurons
Pillai V, Buck L, Lari E
2021, Journal of Experimental Biology, 10.1242/jeb.238147
Exposure to low temperature prepares the turtle brain to withstand anoxic environments during overwintering
Lari E, Buck LT
2021, Journal of Experimental Biology, 10.1242/jeb.242793
Cytoskeletal Arrest: An Anoxia Tolerance Mechanism
Myrka A, Buck L
2021, Metabolites, 10.3390/metabo11080561
2019
Mitochondrial matrix pH acidifies during anoxia and is maintained by the FF-ATPase in anoxia-tolerant painted turtle cortical neurons.
Hawrysh PJ, Buck LT
2019, FEBS open bio, 9, 571-581, 30984533
Oxygen-sensitive interneurons exhibit increased activity and GABA release during ROS scavenging in the cerebral cortex of the western painted turtle
Hawrysh PJ, Buck LT
2019, Journal of Neurophysiology, 10.1152/jn.00104.2019
2018
Taurine activates glycine and GABA receptor currents in anoxia-tolerant painted turtle pyramidal neurons.
Miles AR, Hawrysh PJ, Hossein-Javaheri N, Buck LT
2018, The Journal of experimental biology, 221, 30237241
50 years of comparative biochemistry: The legacy of Peter Hochachka
Buck L, Burness G, Campbell K, Darveau C, Driedzic W, Guderley H, McClelland G, Moon T, Moyes C, Schulte P
2018, Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 10.1016/j.cbpb.2018.02.002
The hypoxia-tolerant vertebrate brain: Arresting synaptic activity
Buck LT, Pamenter ME
2018, Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 10.1016/j.cbpb.2017.11.015
2017
Assessment of anoxia tolerance and photoperiod dependence of GABAergic polarity in the pond snail Lymnaea stagnalis
Buck LT, Bond HC, Malik A
2017, Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 10.1016/j.cbpa.2016.09.016
2016
Stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and GABA receptor inhibition.
Hossein-Javaheri N, Wilkie MP, Lado WE, Buck LT
2017, The Journal of experimental biology, 220, 695-704, 27923876
Assessment of anoxia tolerance and photoperiod dependence of GABAergic polarity in the pond snail Lymnaea stagnalis.
Buck LT, Bond HC, Malik A
2017, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 203, 193-200, 27664385
Phosphorylation of the mitochondrial ATP-sensitive potassium channel occurs independently of PKCε in turtle brain.
Hawrysh PJ, Miles AR, Buck LT
2016, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 200, 44-53, 27280321
Stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and GABA receptor inhibition
Hossein-Javaheri N, Wilkie MP, Lado WE, Buck LT
2016, Journal of Experimental Biology, 10.1242/jeb.146605
2015
Decreases in mitochondrial reactive oxygen species initiate GABAA receptor-mediated electrical suppression in anoxia-tolerant turtle neurons
Hogg DW, Pamenter ME, Dukoff DJ, Buck LT
2015, The Journal of physiology, 25781154
Proteomic changes in the brain of the western painted turtle (Chrysemys picta bellii) during exposure to anoxia
Smith RW, Cash P, Hogg DW, Buck LT
2015, Proteomics, 25583675
Transcriptomic Responses of the Heart and Brain to Anoxia in the Western Painted Turtle.
Keenan SW, Hill CA, Kandoth C, Buck LT, Warren DE
2015, PloS one, 10, e0131669, 26147940
Sensing and surviving hypoxia in vertebrates.
Jonz MG, Buck LT, Perry SF, Schwerte T, Zaccone G
2016, Annals of the New York Academy of Sciences, 1365, 43-58, 25959851
2014
Scavenging ROS dramatically increases NMDA receptor whole cell currents in painted turtle cortical neurons
Dukoff DJ, Hogg DW, Hawrsyh PJ, Buck LT
2014, The Journal of experimental biology, 25063855
Environmental remodelling of GABAergic and glutamatergic neurotransmission: rise of the anoxia-tolerant turtle brain
Hogg DW, Hawrysh PJ, Buck LT
2014, Journal of thermal biology, 44, 85-92, 25086978
2013
Anoxia-mediated calcium release through the mitochondrial permeability transition pore silences NMDA receptor currents in turtle neurons
Hawrysh PJ, Buck LT
2013, The Journal of experimental biology, 216, 4375-87, 24259257
The western painted turtle genome, a model for the evolution of extreme physiological adaptations in a slowly evolving lineage
Bradley Shaffer H, Minx P, Warren DE, Shedlock AM, Thomson RC, Valenzuela N, Abramyan J, Amemiya CT, Badenhorst D, Biggar KK, Borchert GM, Botka CW, Bowden RM, Braun EL, Bronikowski AM, Bruneau BG, Buck LT, Capel B, Castoe TA, Czerwinski M, Delehaunty KD, Edwards SV, Fronick CC, Fujita MK, Fulton L, Graves TA, Green RE, Haerty W, Hariharan R, Hernandez O, Hillier LW, Holloway AK, Janes D, Janzen FJ, Kandoth C, Kong L, de Koning AJ, Li Y, Literman R, McGaugh SE, Mork L, O'Laughlin M, Paitz RT, Pollock DD, Ponting CP, Radhakrishnan S, Raney BJ, Richman JM, St John J, Schwartz T, Sethuraman A, Spinks PQ, Storey KB, Thane N, Vinar T, Zimmerman LM, Warren WC, Mardis ER, Wilson RK
2013, Genome biology, 14, R28, 23537068
Oxygen-sensitive reduction in Ca²⁺-activated K⁺ channel open probability in turtle cerebrocortex
Rodgers-Garlick CI, Hogg DW, Buck LT
2013, Neuroscience, 237, 243-54, 23384611
2012
Painted turtle cortex is resistant to an in vitro mimic of the ischemic mammalian penumbra
Pamenter ME, Hogg DW, Gu XQ, Buck LT, Haddad GG
2012, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 32, 2033-43, 22805876
Oxygen sensitive synaptic neurotransmission in anoxia-tolerant turtle cerebrocortex
Buck LT, Hogg DW, Rodgers-Garlick C, Pamenter ME
2012, Advances in experimental medicine and biology, 758, 71-9, 23080145
2011
The relationship between NMDA receptor function and the high ammonia tolerance of anoxia-tolerant goldfish
Wilkie MP, Pamenter ME, Duquette S, Dhiyebi H, Sangha N, Skelton G, Smith MD, Buck LT
2011, The Journal of experimental biology, 214, 4107-20, 22116753
Endogenous GABA(A) and GABA(B) receptor-mediated electrical suppression is critical to neuronal anoxia tolerance
Pamenter ME, Hogg DW, Ormond J, Shin DS, Woodin MA, Buck LT
2011, Proceedings of the National Academy of Sciences of the United States of America, 108, 11274-9, 21690381
2010
Regulation of AMPA receptor currents by mitochondrial ATP-sensitive K+ channels in anoxic turtle neurons
Zivkovic G, Buck LT
2010, Journal of neurophysiology, 104, 1913-22, 20685922
Adenosinergic modulation of neuronal activity in the pond snail Lymnaea stagnalis
Malik A, Buck LT
2010, The Journal of experimental biology, 213, 1126-32, 20228349
2009
Matching cellular metabolic supply and demand in energy-stressed animals
Staples JF, Buck LT
2009, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 153, 95-105, 19535026
2008
delta-Opioid receptor antagonism induces NMDA receptor-dependent excitotoxicity in anoxic turtle cortex
Pamenter ME, Buck LT
2008, The Journal of experimental biology, 211, 3512-7, 18931323
Evidence of anoxia-induced channel arrest in the brain of the goldfish (Carassius auratus)
Wilkie MP, Pamenter ME, Alkabie S, Carapic D, Shin DS, Buck LT
2008, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 148, 355-62, 18620076
Endogenous reductions in N-methyl-d-aspartate receptor activity inhibit nitric oxide production in the anoxic freshwater turtle cortex
Pamenter ME, Hogg DW, Buck LT
2008, FEBS letters, 582, 1738-42, 18466771
Neuronal membrane potential is mildly depolarized in the anoxic turtle cortex
Pamenter ME, Buck LT
2008, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 150, 410-4, 18519169
Adenosine A1 receptor activation mediates NMDA receptor activity in a pertussis toxin-sensitive manner during normoxia but not anoxia in turtle cortical neurons
Pamenter ME, Shin DS, Buck LT
2008, Brain research, 1213, 27-34, 18455705
2007
Mitochondrial ATP-sensitive K+ channels regulate NMDAR activity in the cortex of the anoxic western painted turtle
Pamenter ME, Shin DS, Cooray M, Buck LT
2008, The Journal of physiology, 586, 1043-58, 18079161
AMPA receptors undergo channel arrest in the anoxic turtle cortex
Pamenter ME, Shin DS, Buck LT
2008, American journal of physiology. Regulatory, integrative and comparative physiology, 294, R606-13, 18056983
Anoxia-induced changes in reactive oxygen species and cyclic nucleotides in the painted turtle
Pamenter ME, Richards MD, Buck LT
2007, Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 177, 473-81, 17347830
Hypoxia tolerance in reptiles, amphibians, and fishes: life with variable oxygen availability
Bickler PE, Buck LT
2007, Annual review of physiology, 69, 145-70, 17037980
2006
Excitatory actions of GABA mediate severe-hypoxia-induced depression of neuronal activity in the pond snail (Lymnaea stagnalis)
Cheung U, Moghaddasi M, Hall HL, Smith JJ, Buck LT, Woodin MA
2006, The Journal of experimental biology, 209, 4429-35, 17079713
Piscine insights into comparisons of anoxia tolerance, ammonia toxicity, stroke and hepatic encephalopathy
Walsh PJ, Veauvy CM, McDonald MD, Pamenter ME, Buck LT, Wilkie MP
2007, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 147, 332-43, 17046301
2005
Calcium and protein phosphatase 1/2A attenuate N-methyl-D-aspartate receptor activity in the anoxic turtle cortex
Shin DS, Wilkie MP, Pamenter ME, Buck LT
2005, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 142, 50-7, 16139540
2004
Adenosine as a signal for ion channel arrest in anoxia-tolerant organisms
Buck LT
2004, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 139, 401-14, 15544964
Time-dependent expression of heat shock proteins 70 and 90 in tissues of the anoxic western painted turtle
Ramaglia V, Buck LT
2004, The Journal of experimental biology, 207, 3775-84, 15371485
Bacterial infection and tissue-specific Hsp72, -73 and -90 expression in western painted turtles
Ramaglia V, Harapa GM, White N, Buck LT
2004, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 138, 139-48, 15450861
2003
Gap junctions do not underlie changes in whole-cell conductance in anoxic turtle brain
Shin DS, Ghai H, Cain SW, Buck LT
2003, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 134, 181-94, 12507621
Effect of anoxia and pharmacological anoxia on whole-cell NMDA receptor currents in cortical neurons from the western painted turtle
Shin DS, Buck LT
2003, Physiological and biochemical zoology : PBZ, 76, 41-51, 12695985
Tissue-specific expression of inducible and constitutive Hsp70 isoforms in the western painted turtle
Scott MA, Locke M, Buck LT
2003, The Journal of experimental biology, 206, 303-11, 12477900