Rodrigo Fernandez-Gonzalez
Associate Professor
Campus
St. George (downtown)
CSB Appointment
Cross
Research Areas
Cell Biology, Developmental Biology, Quantitative Biology /Modelling
Education
Ph.D. University of California, Berkeley and San Francisco 2006
B.Sc. Universidad Autonoma, Madrid, Spain 2000
Titles and Honors
Canada Research Chair in Quantitative Cell Biology and Morphogenesis
Primary Undergraduate Department
Institute of Biomaterials and Biomedical Engineering (IBBME)
Graduate Programs
Cell & Systems Biology
Ecology & Evolutionary Biology
Research Description
Cytoskeletal dynamics and mechanical forces in the assembly and repair of epithelial tissues.
Contact Information
Office Phone: 416-978-7368
Office: MaRS 2 - West Tower, 1411
Lab Phone: 416-978-5911
Email
Mailing Address
MaRS 2 – West Tower
661 University Avenue, 14th Floor
Toronto, Ontario, M5G 1M1
Institute of Biomaterials & Biomedical Engineering
University of Toronto
164 College St.
Toronto, Ontario, M5S 3G9
Canada
Recent News
Publications
Laser ablation to investigate cell and tissue mechanics in vivo. Zulueta-Coarasa T, Fernandez-Gonzalez R. Integrative Mechanobiology: Micro and Nano Techniques in Cell Mechanobiology (edited by Y Sun, DH Kim and C Simmons). Cambridge University Press. 2015.
2022
The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis
Sheppard L, Green DG, Lerchbaumer G, Rothenberg KE, Fernandez-Gonzalez R, Tepass U
2022, Journal of Cell Biology, 10.1083/jcb.202108091
2021
The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium.
Willoughby PM, Allen M, Yu J, Korytnikov R, Chen T, Liu Y, So I, Macpherson N, Mitchell JA, Fernandez-Gonzalez R, Bruce AE
2021, eLife, 10, 33755014
p38-mediated cell growth and survival drive rapid embryonic wound repair
Scepanovic G, Hunter MV, Kafri R, Fernandez-Gonzalez R
2021, Cell Reports, 10.1016/j.celrep.2021.109874
2020
REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects
Lee S, Hadipour-Lakmehsari S, Murthy HR, Gibb N, Miyake T, Teng ACT, Cosme J, Yu JC, Moon M, Lim S, Wong V, Liu P, Billia F, Fernandez-Gonzalez R, Stagljar I, Sharma P, Kislinger T, Scott IC, Gramolini AO
2020, Nature Communications, 10.1038/s41467-019-14143-9
DDR1 (Discoidin Domain Receptor-1)-RhoA (Ras Homolog Family Member A) Axis Senses Matrix Stiffness to Promote Vascular Calcification
Ngai D, Lino M, Rothenberg KE, Simmons CA, Fernandez-Gonzalez R, Bendeck MP
2020, Arteriosclerosis, Thrombosis, and Vascular Biology, 10.1161/ATVBAHA.120.314697
2019
Forceful closure: cytoskeletal networks in embryonic wound repair
Rothenberg KE, Fernandez-Gonzalez R, Bement W
2019, Molecular Biology of the Cell, 10.1091/mbc.E18-04-0248
The Crk adapter protein is essential for Drosophila embryogenesis, where it regulates multiple actin-dependent morphogenic events
Spracklen AJ, Thornton-Kolbe EM, Bonner AN, Florea A, Compton PJ, Fernandez-Gonzalez R, Peifer M, Fehon R
2019, Molecular Biology of the Cell, 10.1091/mbc.E19-05-0302
Actin and myosin dynamics are independent during Drosophila embryonic wound repair
Kobb AB, Rothenberg KE, Fernandez-Gonzalez R, Fehon R
2019, Molecular Biology of the Cell, 10.1091/mbc.E18-11-0703
Role of α-Catenin and its mechanosensing properties in regulating Hippo/YAP-dependent tissue growth
Peifer M, Sarpal R, Yan V, Kazakova L, Sheppard L, Yu JC, Fernandez-Gonzalez R, Tepass U
2019, PLOS Genetics, 10.1371/journal.pgen.1008454
2018
Collision of Expanding Actin Caps with Actomyosin Borders for Cortical Bending and Mitotic Rounding in a Syncytium
Zhang Y, Yu JC, Jiang T, Fernandez-Gonzalez R, Harris TJ
2018, Developmental Cell, 10.1016/j.devcel.2018.04.024
Dynamic force patterns promote collective cell movements during embryonic wound repair
Zulueta-Coarasa T, Fernandez-Gonzalez R
2018, Nature Physics, 10.1038/s41567-018-0111-2
Modeling cell intercalation during Drosophila germband extension
Siang LC, Fernandez-Gonzalez R, Feng JJ
2018, Physical Biology, 10.1088/1478-3975/aad865
Oxidative Stress Orchestrates Cell Polarity to Promote Embryonic Wound Healing
Hunter MV, Willoughby PM, Bruce AE, Fernandez-Gonzalez R
2018, Developmental Cell, 10.1016/j.devcel.2018.10.013
Force-dependent allostery of the α-catenin actin-binding domain controls adherens junction dynamics and functions
Ishiyama N, Sarpal R, Wood MN, Barrick SK, Nishikawa T, Hayashi H, Kobb AB, Flozak AS, Yemelyanov A, Fernandez-Gonzalez R, Yonemura S, Leckband DE, Gottardi CJ, Tepass U, Ikura M
2018, Nature Communications, 10.1038/s41467-018-07481-7
Oriented Cell Division: The Pull of the Pole
Scepanovic G, Fernandez-Gonzalez R
2018, Developmental Cell, 10.1016/j.devcel.2018.11.040
Oxidative Stress Orchestrates Cell Polarity to Promote Embryonic Wound Healing
Hunter MV, Willoughby PM, Bruce AE, Fernandez-Gonzalez R
2018, Developmental Cell, 10.1016/j.devcel.2018.10.013
2017
Tension (re)builds: Biophysical mechanisms of embryonic wound repair
Zulueta-Coarasa T, Fernandez-Gonzalez R
2017, Mechanisms of Development, 10.1016/j.mod.2016.11.004
Tension regulates myosin dynamics duringDrosophilaembryonic wound repair
Kobb AB, Zulueta-Coarasa T, Fernandez-Gonzalez R
2017, Journal of Cell Science, 10.1242/jcs.196139
Automated cell tracking identifies mechanically oriented cell divisions duringDrosophilaaxis elongation
Wang MFZ, Hunter MV, Wang G, McFaul C, Yip CM, Fernandez-Gonzalez R
2017, Development, 10.1242/dev.141473
Cell–cell and cell–extracellular matrix adhesions cooperate to organize actomyosin networks and maintain force transmission during dorsal closure
Goodwin K, Lostchuck EE, Cramb KML, Zulueta-Coarasa T, Fernandez-Gonzalez R, Tanentzapf G, Fehon R
2017, Molecular Biology of the Cell, 10.1091/mbc.E17-01-0033
Myosin II promotes the anisotropic loss of the apical domain during Drosophila neuroblast ingression
Tepass U, Fernandez-Gonzalez R, Wang MF, Oh Y, Simões S
2017, Journal of Cell Biology, 10.1083/jcb.201608038
Shape of my heart: Cell-cell adhesion and cytoskeletal dynamics during Drosophila cardiac morphogenesis
McFaul CM, Fernandez-Gonzalez R
2017, Experimental Cell Research, 10.1016/j.yexcr.2017.03.057
An Actomyosin-Arf-GEF Negative Feedback Loop for Tissue Elongation under Stress
West JJ, Zulueta-Coarasa T, Maier JA, Lee DM, Bruce AE, Fernandez-Gonzalez R, Harris TJ
2017, Current Biology, 10.1016/j.cub.2017.06.038
2016
Local mechanical forces promote polarized junctional assembly and axis elongation in Drosophila
Yu JC, Fernandez-Gonzalez R
2016, eLife, 10.7554/eLife.10757
Basal Cell-Extracellular Matrix Adhesion Regulates Force Transmission during Tissue Morphogenesis
Goodwin K, Ellis S, Lostchuck E, Zulueta-Coarasa T, Fernandez-Gonzalez R, Tanentzapf G
2016, Developmental Cell, 10.1016/j.devcel.2016.11.003
2015
An in vitro model of tissue boundary formation for dissecting the contribution of different boundary forming mechanisms
Javaherian S, D\'Arcangelo E, Slater B, Zulueta-Coarasa T, Fernandez-Gonzalez R, McGuigan AP
2015, Integrative Biology, 10.1039/c4ib00272e
Anisotropic stress orients remodelling of mammalian limb bud ectoderm
Lau K, Tao H, Liu H, Wen J, Sturgeon K, Sorfazlian N, Lazic S, Burrows JTA, Wong MD, Li D, Deimling S, Ciruna B, Scott I, Simmons C, Henkelman RM, Williams T, Hadjantonakis A, Fernandez-Gonzalez R, Sun Y, Hopyan S
2015, Nature Cell Biology, 10.1038/ncb3156
Polarized E-cadherin endocytosis directs actomyosin remodeling during embryonic wound repair
Fernandez-Gonzalez R, Harris TJ, Lee DM, Hunter MV
2015, Journal of Cell Biology, 10.1083/jcb.201501076
A biomechanical model for cell polarization and intercalation duringDrosophilagermband extension
Lan H, Wang Q, Fernandez-Gonzalez R, Feng JJ
2015, Physical Biology, 10.1088/1478-3975/12/5/056011
2014
Automated multidimensional image analysis reveals a role for Abl in embryonic wound repair
Zulueta-Coarasa T, Tamada M, Lee EJ, Fernandez-Gonzalez R
2014, Development (Cambridge, England), 141, 2901-11, 24948602
Automated multidimensional image analysis reveals a role for Abl in embryonic wound repair
Zulueta-Coarasa T, Tamada M, Lee EJ, Fernandez-Gonzalez R
2014, Development, 10.1242/dev.106898
Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis
Leung CYB, Fernandez-Gonzalez R
2014, , 10.1007/978-1-4939-1164-6_7
2013
Gastrulation: cell polarity comes full circle
Hunter MV, Fernandez-Gonzalez R
2013, Current biology : CB, 23, R845-8, 24070446
Wounded cells drive rapid epidermal repair in the early Drosophila embryo
Fernandez-Gonzalez R, Zallen JA
2013, Molecular biology of the cell, 24, 3227-37, 23985320
Wounded cells drive rapid epidermal repair in the early Drosophila embryo
Fernandez-Gonzalez R, Zallen JA, Fehon R
2013, Molecular Biology of the Cell, 10.1091/mbc.E13-05-0228
Gastrulation: Cell Polarity Comes Full Circle
Hunter M, Fernandez-Gonzalez R
2013, Current Biology, 10.1016/j.cub.2013.08.003
2012
Feeling the squeeze: live-cell extrusion limits cell density in epithelia
Fernandez-Gonzalez R, Zallen JA
2012, Cell, 149, 965-7, 22632965
2011
Oscillatory behaviors and hierarchical assembly of contractile structures in intercalating cells
Fernandez-Gonzalez R, Zallen JA
2011, Physical biology, 8, 045005, 21750365
2010
3D reconstruction of histological sections: Application to mammary gland tissue
Arganda-Carreras I, Fernández-González R, Muñoz-Barrutia A, Ortiz-De-Solorzano C
2010, Microscopy research and technique, 73, 1019-29, 20232465
Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation
Simões Sde M, Blankenship JT, Weitz O, Farrell DL, Tamada M, Fernandez-Gonzalez R, Zallen JA
2010, Developmental cell, 19, 377-88, 20833361
Integration of contractile forces during tissue invagination
Martin AC, Gelbart M, Fernandez-Gonzalez R, Kaschube M, Wieschaus EF
2010, The Journal of cell biology, 188, 735-49, 20194639
Use of stem cell markers in dissociated mammary populations
Shelton DN, Fernandez-Gonzalez R, Illa-Bochaca I, Ortiz-de-Solorzano C, Barcellos-Hoff MH, Welm BE
2010, Methods in molecular biology (Clifton, N.J.), 621, 49-55, 20405358
Limiting-dilution transplantation assays in mammary stem cell studies
Illa-Bochaca I, Fernandez-Gonzalez R, Shelton DN, Welm BE, Ortiz-de-Solorzano C, Barcellos-Hoff MH
2010, Methods in molecular biology (Clifton, N.J.), 621, 29-47, 20405357
In situ analysis of cell populations: long-term label-retaining cells
Fernandez-Gonzalez R, Illa-Bochaca I, Shelton DN, Welm BE, Barcellos-Hoff MH, Ortiz-de-Solorzano C
2010, Methods in molecular biology (Clifton, N.J.), 621, 1-28, 20405356
2009
Cell mechanics and feedback regulation of actomyosin networks
Fernandez-Gonzalez R, Zallen JA
2009, Science signaling, 2, pe78, 20009102
Myosin II dynamics are regulated by tension in intercalating cells
Fernandez-Gonzalez R, Simoes Sde M, Röper JC, Eaton S, Zallen JA
2009, Developmental cell, 17, 736-43, 19879198
Q&A: quantitative approaches to planar polarity and tissue organization
Marcinkevicius E, Fernandez-Gonzalez R, Zallen JA
2009, Journal of biology, 8, 103, 20064196
2008
Mapping mammary gland architecture using multi-scale in situ analysis
Fernandez-Gonzalez R, Illa-Bochaca I, Welm BE, Fleisch MC, Werb Z, Ortiz-de-Solorzano C, Barcellos-Hoff MH
2009, Integrative biology : quantitative biosciences from nano to macro, 1, 80-9, 20023794
Epithelial organization: may the force be with you
Fernandez-Gonzalez R, Zallen JA
2008, Current biology : CB, 18, R163-5, 18302918
2006
Quantitative in vivo microscopy: the return from the ‘omics’
Fernández-González R, Muñoz-Barrutia A, Barcellos-Hoff MH, Ortiz-de-Solorzano C
2006, Current opinion in biotechnology, 17, 501-10, 16899361
High-throughput analysis of multispectral images of breast cancer tissue
Adiga U, Malladi R, Fernandez-Gonzalez R, Ortiz de Solorzano C
2006, IEEE transactions on image processing : a publication of the IEEE Signal Processing Society, 15, 2259-68, 16900681
2005
A tool for the quantitative spatial analysis of complex cellular systems
Fernandez-Gonzalez R, Barcellos-Hoff MH, Ortiz-de-Solórzano C
2005, IEEE transactions on image processing : a publication of the IEEE Signal Processing Society, 14, 1300-13, 16190466
2004
Quantitative image analysis in mammary gland biology
Fernandez-Gonzalez R, Barcellos-Hoff MH, Ortiz-de-Solórzano C
2004, Journal of mammary gland biology and neoplasia, 9, 343-59, 15838604
A tool for the quantitative spatial analysis of mammary gland epithelium
Fernandez-Gonzalez R, de Solorzano CO
2004, Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, 2, 1549-52, 17271993