Voula Kanelis

Associate Professor



CSB Appointment


Research Areas

Structural Biology


Ph.D. University of Toronto 2001
B.Sc. University of Western Ontario 1995

Titles and Honors

CIHR New Investigator (2012)

Primary Undergraduate Department

Chemistry, UTM

Graduate Programs

Cell & Systems Biology

Research Description

We study nucleotide binding domains (NBDs) from the sulfonylurea receptors (SURs). The SUR proteins, which are members of the ATP binding cassette (ABC) superfamily of proteins, form regulatory domains of ATP sensitive K+ channels. Mutations in the NBDs of the SUR proteins result in diabetes, hyperinsulinism, and cardiac disorders.

Contact Information

Office Phone: 905-569-4542
Office: DV4042

Mailing Address

Department of Cell & Systems Biology
University of Toronto
1265 Military Trail
Scarborough, ON M1C 1A4

Visit lab’s website



Successful development and use of a thermodynamic stability screen for optimizing the yield of nucleotide binding domains

de Araujo ED, Kanelis V
2014, Protein expression and purification, 103, 38-47, 25153533

HNH proteins are a widespread component of phage DNA packaging machines

Kala S, Cumby N, Sadowski PD, Hyder BZ, Kanelis V, Davidson AR, Maxwell KL
2014, Proceedings of the National Academy of Sciences of the United States of America, 111, 6022-7, 24711378

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NMR and fluorescence studies of drug binding to the first nucleotide binding domain of SUR2A

López-Alonso JP, de Araujo ED, Kanelis V
2012, Biochemistry, 51, 9211-22, 23078514

The protein gp74 from the bacteriophage HK97 functions as a HNH endonuclease

Moodley S, Maxwell KL, Kanelis V
2012, Protein science : a publication of the Protein Society, 21, 809-18, 22434504

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The first nucleotide binding domain of the sulfonylurea receptor 2A contains regulatory elements and is folded and functions as an independent module

de Araujo ED, Ikeda LK, Tzvetkova S, Kanelis V
2011, Biochemistry, 50, 6655-66, 21714514

NMR spectroscopy to study the dynamics and interactions of CFTR

Kanelis V, Chong PA, Forman-Kay JD
2011, Methods in molecular biology (Clifton, N.J.), 741, 377-403, 21594798

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The solution structure of the C-terminal Ig-like domain of the bacteriophage λ tail tube protein

Pell LG, Gasmi-Seabrook GM, Morais M, Neudecker P, Kanelis V, Bona D, Donaldson LW, Edwards AM, Howell PL, Davidson AR, Maxwell KL
2010, Journal of molecular biology, 403, 468-79, 20826161

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NMR evidence for differential phosphorylation-dependent interactions in WT and DeltaF508 CFTR

Kanelis V, Hudson RP, Thibodeau PH, Thomas PJ, Forman-Kay JD
2010, The EMBO journal, 29, 263-77, 19927121

The phage lambda major tail protein structure reveals a common evolution for long-tailed phages and the type VI bacterial secretion system

Pell LG, Kanelis V, Donaldson LW, Howell PL, Davidson AR
2009, Proceedings of the National Academy of Sciences of the United States of America, 106, 4160-5, 19251647

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Regulation of Nedd4-2 self-ubiquitination and stability by a PY motif located within its HECT-domain

Bruce MC, Kanelis V, Fouladkou F, Debonneville A, Staub O, Rotin D
2008, The Biochemical journal, 415, 155-63, 18498246

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CFTR regulatory region interacts with NBD1 predominantly via multiple transient helices

Baker JM, Hudson RP, Kanelis V, Choy WY, Thibodeau PH, Thomas PJ, Forman-Kay JD
2007, Nature structural & molecular biology, 14, 738-45, 17660831

Nuclear magnetic resonance (NMR) solution structure, dynamics, and binding properties of the kringle IV type 8 module of apolipoprotein(a)

Chitayat S, Kanelis V, Koschinsky ML, Smith SP
2007, Biochemistry, 46, 1732-42, 17263558

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Structural determinants for high-affinity binding in a Nedd4 WW3* domain-Comm PY motif complex

Kanelis V, Bruce MC, Skrynnikov NR, Rotin D, Forman-Kay JD
2006, Structure (London, England : 1993), 14, 543-53, 16531238

Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy

Tugarinov V, Kanelis V, Kay LE
2006, Nature protocols, 1, 749-54, 17406304

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Probing slow dynamics in high molecular weight proteins by methyl-TROSY NMR spectroscopy: application to a 723-residue enzyme

Korzhnev DM, Kloiber K, Kanelis V, Tugarinov V, Kay LE
2004, Journal of the American Chemical Society, 126, 3964-73, 15038751

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Affinity and specificity of interactions between Nedd4 isoforms and the epithelial Na+ channel

Henry PC, Kanelis V, O'Brien MC, Kim B, Gautschi I, Forman-Kay J, Schild L, Rotin D
2003, The Journal of biological chemistry, 278, 20019-28, 12654927

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