Module: Current Techniques in Gene Regulation
CSB1020H/F, Teaching Section LEC 0134
Instructor
Professor Ho Sung Rhee (hosung.rhee@utoronto.ca)
Offered Fall 2022 (six weeks, Nov. 3rd – Dec. 8th, 2021)
Enrolment limited to 8 graduate students
Schedule
3:10 PM – 6:00 PM, Wednesdays
Weight
One module (0.25 FCE)
Location
Online in Fall 2021
Course Description
This course will examine cutting-edge techniques to study gene regulation. Techniques to be discussed include: cell (re)programming into specific cell types, long-range chromatin interaction analysis, protein-DNA interaction analysis using nuclease (e.g., CUT&RUN), current strategies for genome-editing in vivo and in vitro systems such as CRISPR-Cas9, and identifying gene regulatory networks and elements at the single cell level using next-generation sequencing technologies (e.g., scRNA-seq, snATAC-seq). Lectures and seminars will involve presentations and discussions of current topics in gene regulation. Students will present recently published research articles, lead a discussion, and write a report.
Evaluation
1st seminar presentation: 27%
2nd seminar presentation: 27%
Participation: 22%
Written assignment: 24%
Pre-requisites: Background in cell and molecular biology
Reading materials: Required readings will be primary research articles and reviews, and will be provided during class.
Website: Quercus – https://q.utoronto.ca
Last updated on September 14th, 2021