Current Techniques in Gene Regulation

Module: Current Techniques in Gene Regulation

CSB1020H/F, Teaching Section LEC 0134

Instructor

Professor Ho Sung Rhee (hosung.rhee@utoronto.ca)

Offered Fall 2022 (six weeks, Nov. 3rd – Dec. 8th, 2021)

Enrolment limited to 8 graduate students

Schedule

3:10 PM – 6:00 PM, Wednesdays

Weight

One module (0.25 FCE)

Location

Online in Fall 2021

Course Description

This course will examine cutting-edge techniques to study gene regulation. Techniques to be discussed include: cell (re)programming into specific cell types, long-range chromatin interaction analysis, protein-DNA interaction analysis using nuclease (e.g., CUT&RUN), current strategies for genome-editing in vivo and in vitro systems such as CRISPR-Cas9, and identifying gene regulatory networks and elements at the single cell level using next-generation sequencing technologies (e.g., scRNA-seq, snATAC-seq). Lectures and seminars will involve presentations and discussions of current topics in gene regulation. Students will present recently published research articles, lead a discussion, and write a report.

Evaluation

1st seminar presentation:       27%

2nd seminar presentation:      27%

Participation:                           22%

Written assignment:                24%

Pre-requisites: Background in cell and molecular biology

Reading materials: Required readings will be primary research articles and reviews, and will be provided during class.

Website: Quercus – https://q.utoronto.ca

Last updated on August 11th, 2022