Module: Current Techniques in Gene Regulation

CSB1020H/F, Teaching Section LEC 0134


Professor Ho Sung Rhee (

Offered Fall 2022 (six weeks, Nov. 3rd – Dec. 8th, 2021)

Enrolment limited to 8 graduate students


3:10 PM – 6:00 PM, Wednesdays


One module (0.25 FCE)


Online in Fall 2021

Course Description

This course will examine cutting-edge techniques to study gene regulation. Techniques to be discussed include: cell (re)programming into specific cell types, long-range chromatin interaction analysis, protein-DNA interaction analysis using nuclease (e.g., CUT&RUN), current strategies for genome-editing in vivo and in vitro systems such as CRISPR-Cas9, and identifying gene regulatory networks and elements at the single cell level using next-generation sequencing technologies (e.g., scRNA-seq, snATAC-seq). Lectures and seminars will involve presentations and discussions of current topics in gene regulation. Students will present recently published research articles, lead a discussion, and write a report.


1st seminar presentation:       27%

2nd seminar presentation:      27%

Participation:                           22%

Written assignment:                24%

Pre-requisites: Background in cell and molecular biology

Reading materials: Required readings will be primary research articles and reviews, and will be provided during class.

Website: Quercus –


Last updated on August 11th, 2022