Coordinator
Dr. Pauline Wang
Offered Winter 2025 session
Enrolment limited to 2 graduate students (CSB grads with priority)
Weight
Half credit (0.5 FCE)
Time
Tuesdays 12 pm – 4 pm
Location
St. George campus – Earth Sciences Centre, room 4076
Description
Genomics and proteomics have revolutionized biological research. It is now theoretically possible to fully characterize the structure, organization, regulation and interaction of all genes, proteins and small bioactive molecules in an organism. CSB 1025H/S is an intensive and rigorous laboratory course that will teach students how to produce and analyze data that are central to the fields of genomics and proteomics. The course is divided into three modules, the first of which focuses on genomics, the second on transcriptomics, and the third on proteomics. Each module begins with at least two wet labs where students generate data and end with computer labs where students analyze the data. In this way students will learn how to conduct an experiment from beginning to end. Techniques taught include DNA and RNA extraction, shotgun library construction, PCR, DNA sequencing, expression profiling using microarrays, 2D-gel proteome analysis, mass spectrometry and associated bioinformatics analyses such as sequence analysis and assembly, and statistical analysis of microarray and mass spectrometry data. This is an advanced laboratory and computer-based course, and assumes a strong background in molecular genetics and some prior laboratory experience.
Required Text: No required textbook. Information will be provided through lectures presented in the first wet lab and first computer lab of each module.
Evaluation: Three quizzes (15%), three lab reports (60%), lab performance (25%). Graduate students have an additional grant proposal (20%).
Prerequisite: BIO 260H1/HMB 265H1 (Genetics), BIO 255Y1/CSB 330H1/350H1 or by permission of the instructor. Recommended Preparation: BCH 311H1/CSB 349H1/MGY 311Y1
Last updated on August 9th, 2024