Sergey V. Plotnikov

faculty_img Academic Title: Assistant Professor

Campus: St. George

CSB Appointment: Full

Primary Undergraduate Department:
Cell & Systems Biology

Graduate Programs:
Cell & Systems Biology

Titles and Honors:
Academic or Administrative Appointments:
Education:
Ph.D. Institute of Marine Biology, Vladivostok, Russia

 

Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Harbord St.
Toronto, ON M5S 3G5
Canada

 

Contact Information
Office phone: 416-978-1734
Office: RW332A
Lab: RW330
Lab phone: 416-978-3476
Email: sergey.plotnikov@utoronto.ca
URL:

 

Research Areas
Animal Biology
Cell Biology
Developmental Biology
Quantitative Biology

 

Research

Cell migration is central in a wide range of physiological and pathological processes, including development, wound healing and cancer metastasis. In the animal, cell migration is governed by a variety of extracellular cues that control activity of specific signaling pathways and regulate cytoskeletal dynamics. These cues are diverse in nature and include biochemical gradients, mechanical forces, and gradients of extracellular matrix stiffness and topology. Although several major signaling pathways have been previously implicated as master regulators of cell migration guided by the biochemical cues, the mechanisms by which cells sense mechanical stimuli and transduce them into cellular responses, such as migration, are largely unknown.
We aim to uncover how migration of animal cells is controlled by mechanical signals form the extracellular environment. Our lab uses a multifaceted experimental approach, combining cell biology, molecular biology, and biophysics techniques with high-resolution quantitative optical microscopy to address the following specific questions: (i) unraveling molecular mechanisms that transduce physical signals experienced by cells into activity of signaling pathways, (ii) assess contribution of directed cell migration guided by physical cues to pathological conditions, (iii) understanding how mechanosensitive cellular responses are modulated by chemical guidance cues, such as spatial gradients of growth factors.

 

Publications

2015

Top
Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly.Skau CT, Plotnikov SV, Doyle AD, Waterman CM.Proc. Natl. Acad. Sci. U.S.A. 2015 Apr;
pubmed
Rac1-dependent phosphorylation and focal adhesion recruitment of myosin IIA regulates migration and mechanosensing.Pasapera AM, Plotnikov SV, Fischer RS, Case LB, Egelhoff TT, Waterman CM.Curr. Biol. 2015 Jan;25(2):175-86
pubmed

2014

Top
High-resolution traction force microscopy.Plotnikov SV, Sabass B, Schwarz US, Waterman CM.Methods Cell Biol. 2014;123:367-94
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2013

Top
Vinculin-actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth.Thievessen I, Thompson PM, Berlemont S, Plevock KM, Plotnikov SV, Zemljic-Harpf A, Ross RS, Davidson MW, Danuser G, Campbell SL, Waterman CM.J. Cell Biol. 2013 Jul;202(1):163-77
pubmed
Guiding cell migration by tugging.Plotnikov SV, Waterman CM.Curr. Opin. Cell Biol. 2013 Oct;25(5):619-26
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2012

Top
Force fluctuations within focal adhesions mediate ECM-rigidity sensing to guide directed cell migration.Plotnikov SV, Pasapera AM, Sabass B, Waterman CM.Cell 2012 Dec;151(7):1513-27
pubmed
Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure.Chen X, Nadiarynkh O, Plotnikov S, Campagnola PJ.Nat Protoc 2012 Apr;7(4):654-69
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2009

Top
Cardiac fibroblasts require focal adhesion kinase for normal proliferation and migration.Manso AM, Kang SM, Plotnikov SV, Thievessen I, Oh J, Beggs HE, Ross RS.Am. J. Physiol. Heart Circ. Physiol. 2009 Mar;296(3):H627-38
pubmed

2008

Top
Measurement of muscle disease by quantitative second-harmonic generation imaging.Plotnikov SV, Kenny AM, Walsh SJ, Zubrowski B, Joseph C, Scranton VL, Kuchel GA, Dauser D, Xu M, Pilbeam CC, Adams DJ, Dougherty RP, Campagnola PJ, Mohler WA.J Biomed Opt 2008 Jul-Aug;13(4):044018
pubmed

2007

Top
Second harmonic generation imaging microscopy studies of osteogenesis imperfecta.Nadiarnykh O, Plotnikov S, Mohler WA, Kalajzic I, Redford-Badwal D, Campagnola PJ.J Biomed Opt 2007 Sep-Oct;12(5):051805
pubmed

2006

Top
Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres.Plotnikov SV, Millard AC, Campagnola PJ, Mohler WA.Biophys. J. 2006 Jan;90(2):693-703
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Optical clearing for improved contrast in second harmonic generation imaging of skeletal muscle.Plotnikov S, Juneja V, Isaacson AB, Mohler WA, Campagnola PJ.Biophys. J. 2006 Jan;90(1):328-39
pubmed