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MSc Exit Seminar – Betty Geng (Goring Lab)

December 1, 2020 @ 1:00 pm - 1:30 pm

Investigating the role of BRASSIKIN1 (BKN1) in the compatible pollen-stigma interactions in Arabidopsis species 



The basal compatible pollen response pathway in the stigma is initiated upon recognition of compatible pollen. A previous attempt to identify candidate genes in this signal transduction pathway led to the discovery of two tandemly-linked genes that encode pseudokinases, BRASSIKIN1 (BKN1) and BKN2. Interestingly, Arabidopsis thaliana Col-0 is predicted to encode a truncated BKN1, but A. thaliana Hh-0 and Arabidopsis lyrata BKN1 genes encode ful length proteins. The A. thaliana bkn2 mutant in the Col-0 accession showed a mild pollen hydration defect when wild-type pollen was placed on the mutant stigmas, and the bkn1-bkn2 double mutant did not show additional defects. To further understand the role of BKN1 in the compatible pollen pathway, BKN1 cDNAs from A. lyrata and A. thaliana Hh-0 were used to complement the bkn1-3 bkn2-5 mutant. Additionally, BKN1 deletions were generated in the A. thaliana Hh-0 accession with the CRISPR/Cas9 system. Results show that BKN1 complementation restored the minor pollen hydration defects when wild-type pollen was placed on stigmas from the complementation lines, and no significant fertility defect was seen in A. thaliana Hh-0 bkn1 mutants, both results suggesting the functional redundancy between BKN1 and BKN2.

Join Zoom Meeting

Tuesday, December 1st, 2020 at 1:00pm

Meeting ID: 825 8768 6586

Host: Daphne Goring (d.goring@utoronto.ca)


December 1, 2020
1:00 pm - 1:30 pm