Heather McFarlane

Assistant Professor


Campus

St. George (downtown)

CSB Appointment

Full

Research Areas

Cell Biology, Developmental Biology, Genetics / Genomics, Molecular Biology, Plant Biology, Systems Biology

Education

Ph.D. (Botany), Univeristy of British Columbia, 2013
M.Sc. (Biology), McGill University, 2008
BSc (Cell Biology), University of British Columbia, 2006

Titles and Honors

Canada Research Chair in Plant Cell Biology

Primary Undergraduate Department

Cell & Systems Biology

Graduate Programs

Cell & Systems Biology

Research Description

We ask and answer questions about the fundamental mechanisms by which plants sense their environment through the cell wall and how plants adjust their growth in response to these signals.


Contact Information

Office Phone: 416-978-0523
Office: ESC3071
Lab: ESC3058
Lab Phone: 416-978-0837
Email

Mailing Address

Department of Cell & Systems Biology
University of Toronto
25 Willcocks St.
Toronto, ON M5S 3B2
Canada

Visit lab’s website


Publications

2020

EPSIN1 and MTV1 define functionally overlapping but molecularly distinct trans-Golgi network subdomains in Arabidopsis

Heinze L, Freimuth N, Rößling A, Hahnke R, Riebschläger S, Fröhlich A, Sampathkumar A, McFarlane HE, Sauer M
2020, Proceedings of the National Academy of Sciences, 10.1073/pnas.2004822117

The Cytoskeleton and Its Role in Determining Cellulose Microfibril Angle in Secondary Cell Walls of Woody Tree Species

Tobias , Spokevicius , McFarlane , Bossinger
2020, Plants, 10.3390/plants9010090

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2019

Separating Golgi proteins from cis to trans reveals underlying properties of cisternal localization.

Parsons HT, Stevens TJ, McFarlane HE, Vidal-Melgosa S, Griss J, Lawrence N, Butler R, Sousa MML, Salemi M, Willats WGT, Petzold CJ, Heazlewood JL, Lilley KS
2019, The Plant cell, 31266899

Separating Golgi proteins from cis to trans reveals underlying properties of cisternal localization.

Parsons HT, Stevens TJ, McFarlane HE, Vidal-Melgosa S, Griss J, Lawrence N, Butler R, Sousa MML, Salemi M, Willats WGT, Petzold CJ, Heazlewood JL, Lilley KS
2019, The Plant cell, 31266899

The companion of cellulose synthase 1 confers salt tolerance through a Tau-like mechanism in plants.

Kesten C, Wallmann A, Schneider R, McFarlane HE, Diehl A, Khan GA, van Rossum BJ, Lampugnani ER, Szymanski WG, Cremer N, Schmieder P, Ford KL, Seiter F, Heazlewood JL, Sanchez-Rodriguez C, Oschkinat H, Persson S
2019, Nature communications, 10, 857, 30787279

A GDSL Esterase/Lipase Catalyzes the Esterification of Lutein in Bread Wheat

Watkins JL, Li M, McQuinn RP, Chan KX, McFarlane HE, Ermakova M, Furbank RT, Mares D, Dong C, Chalmers KJ, Sharp P, Mather DE, Pogson BJ
2019, The Plant Cell, 10.1105/tpc.19.00272

Separating Golgi Proteins from Cis to Trans Reveals Underlying Properties of Cisternal Localization

Parsons HT, Stevens TJ, McFarlane HE, Vidal-Melgosa S, Griss J, Lawrence N, Butler R, Sousa MM, Salemi M, Willats WG, Petzold CJ, Heazlewood JL, Lilley KS
2019, The Plant Cell, 10.1105/tpc.19.00081

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2018

A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids.

Ebert B, Rautengarten C, McFarlane HE, Rupasinghe T, Zeng W, Ford K, Scheller HV, Bacic A, Roessner U, Persson S, Heazlewood JL
2018, Nature plants, 4, 792-801, 30224661

Inhibition of TOR Represses Nutrient Consumption, Which Improves Greening after Extended Periods of Etiolation.

Zhang Y, Zhang Y, McFarlane HE, Obata T, Richter AS, Lohse M, Grimm B, Persson S, Fernie AR, Giavalisco P
2018, Plant physiology, 178, 101-117, 30049747

Cellulose synthesis during cell plate assembly.

Chen HW, Persson S, Grebe M, McFarlane HE
2018, Physiologia plantarum, 164, 17-26, 29418000

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2017

Two Complementary Mechanisms Underpin Cell Wall Patterning during Xylem Vessel Development.

Schneider R, Tang L, Lampugnani ER, Barkwill S, Lathe R, Zhang Y, McFarlane HE, Pesquet E, Niittyla T, Mansfield SD, Zhou Y, Persson S
2017, The Plant cell, 29, 2433-2449, 28947492

The elaborate route for UDP-arabinose delivery into the Golgi of plants.

Rautengarten C, Birdseye D, Pattathil S, McFarlane HE, Saez-Aguayo S, Orellana A, Persson S, Hahn MG, Scheller HV, Heazlewood JL, Ebert B
2017, Proceedings of the National Academy of Sciences of the United States of America, 114, 4261-4266, 28373556

Multiscale Structural Analysis of Plant ER-PM Contact Sites.

McFarlane HE, Lee EK, van Bezouwen LS, Ross B, Rosado A, Samuels AL
2017, Plant & cell physiology, 58, 478-484, 28100648

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2016

Cell cycle-regulated PLEIADE/AtMAP65-3 links membrane and microtubule dynamics during plant cytokinesis.

Steiner A, Rybak K, Altmann M, McFarlane HE, Klaeger S, Nguyen N, Facher E, Ivakov A, Wanner G, Kuster B, Persson S, Braun P, Hauser MT, Assaad FF
2016, The Plant journal : for cell and molecular biology, 88, 531-541, 27420177

Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis.

Zhang Y, Nikolovski N, Sorieul M, Vellosillo T, McFarlane HE, Dupree R, Kesten C, Schneider R, Driemeier C, Lathe R, Lampugnani E, Yu X, Ivakov A, Doblin MS, Mortimer JC, Brown SP, Persson S, Dupree P
2016, Nature communications, 7, 11656, 27277162

Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings.

de Michele R, McFarlane HE, Parsons HT, Meents MJ, Lao J, González Fernández-Niño SM, Petzold CJ, Frommer WB, Samuels AL, Heazlewood JL
2016, Journal of proteome research, 15, 900-13, 26781341

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2015

The impact of abiotic factors on cellulose synthesis.

Wang T, McFarlane HE, Persson S
2016, Journal of experimental botany, 67, 543-52, 26552883

A cascade of sequentially expressed sucrose transporters in the seed coat and endosperm provides nutrition for the Arabidopsis embryo.

Chen LQ, Lin IW, Qu XQ, Sosso D, McFarlane HE, Londoño A, Samuels AL, Frommer WB
2015, The Plant cell, 27, 607-19, 25794936

A cascade of sequentially expressed sucrose transporters in the seed coat and endosperm provides nutrition for the Arabidopsis embryo.

Chen LQ, Lin IW, Qu XQ, Sosso D, McFarlane HE, Londoño A, Samuels AL, Frommer WB
2015, The Plant cell, 27, 607-19, 25794936

The Arabidopsis synaptotagmin1 is enriched in endoplasmic reticulum-plasma membrane contact sites and confers cellular resistance to mechanical stresses.

Pérez-Sancho J, Vanneste S, Lee E, McFarlane HE, Esteban Del Valle A, Valpuesta V, Friml J, Botella MA, Rosado A
2015, Plant physiology, 168, 132-43, 25792253

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2014

The cell biology of cellulose synthesis.

McFarlane HE, Döring A, Persson S
2014, Annual review of plant biology, 65, 69-94, 24579997

The cell biology of cellulose synthesis.

McFarlane HE, Döring A, Persson S
2014, Annual review of plant biology, 65, 69-94, 24579997

Golgi- and trans-Golgi network-mediated vesicle trafficking is required for wax secretion from epidermal cells.

McFarlane HE, Watanabe Y, Yang W, Huang Y, Ohlrogge J, Samuels AL
2014, Plant physiology, 164, 1250-60, 24468625

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2013

Cell wall polysaccharides are mislocalized to the Vacuole in echidna mutants.

McFarlane HE, Watanabe Y, Gendre D, Carruthers K, Levesque-Tremblay G, Haughn GW, Bhalerao RP, Samuels L
2013, Plant & cell physiology, 54, 1867-80, 24058145

Cell wall polysaccharides are mislocalized to the Vacuole in echidna mutants.

McFarlane HE, Watanabe Y, Gendre D, Carruthers K, Levesque-Tremblay G, Haughn GW, Bhalerao RP, Samuels L
2013, Plant & cell physiology, 54, 1867-80, 24058145

Cell wall polysaccharides are mislocalized to the Vacuole in echidna mutants.

McFarlane HE, Watanabe Y, Gendre D, Carruthers K, Levesque-Tremblay G, Haughn GW, Bhalerao RP, Samuels L
2013, Plant & cell physiology, 54, 1867-80, 24058145

ECHIDNA-mediated post-Golgi trafficking of auxin carriers for differential cell elongation.

Boutté Y, Jonsson K, McFarlane HE, Johnson E, Gendre D, Swarup R, Friml J, Samuels L, Robert S, Bhalerao RP
2013, Proceedings of the National Academy of Sciences of the United States of America, 110, 16259-64, 24043780

Trans-Golgi network localized ECHIDNA/Ypt interacting protein complex is required for the secretion of cell wall polysaccharides in Arabidopsis.

Gendre D, McFarlane HE, Johnson E, Mouille G, Sjödin A, Oh J, Levesque-Tremblay G, Watanabe Y, Samuels L, Bhalerao RP
2013, The Plant cell, 25, 2633-46, 23832588

Patterning and lifetime of plasma membrane-localized cellulose synthase is dependent on actin organization in Arabidopsis interphase cells.

Sampathkumar A, Gutierrez R, McFarlane HE, Bringmann M, Lindeboom J, Emons AM, Samuels L, Ketelaar T, Ehrhardt DW, Persson S
2013, Plant physiology, 162, 675-88, 23606596

Patterning and lifetime of plasma membrane-localized cellulose synthase is dependent on actin organization in Arabidopsis interphase cells.

Sampathkumar A, Gutierrez R, McFarlane HE, Bringmann M, Lindeboom J, Emons AM, Samuels L, Ketelaar T, Ehrhardt DW, Persson S
2013, Plant physiology, 162, 675-88, 23606596

Mitochondrial AtPAM16 is required for plant survival and the negative regulation of plant immunity.

Huang Y, Chen X, Liu Y, Roth C, Copeland C, McFarlane HE, Huang S, Lipka V, Wiermer M, Li X
2013, Nature communications, 4, 2558, 24153405

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2012

RDR1 and SGS3, components of RNA-mediated gene silencing, are required for the regulation of cuticular wax biosynthesis in developing inflorescence stems of Arabidopsis.

Lam P, Zhao L, McFarlane HE, Aiga M, Lam V, Hooker TS, Kunst L
2012, Plant physiology, 159, 1385-95, 22689894

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2011

Plant cell wall secretion and lipid traffic at membrane contact sites of the cell cortex.

Samuels L, McFarlane HE
2012, Protoplasma, S19-23, 22160188

Cellulose synthesis via the FEI2 RLK/SOS5 pathway and cellulose synthase 5 is required for the structure of seed coat mucilage in Arabidopsis.

Harpaz-Saad S, McFarlane HE, Xu S, Divi UK, Forward B, Western TL, Kieber JJ
2011, The Plant journal : for cell and molecular biology, 68, 941-53, 21883548

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2010

Arabidopsis ABCG transporters, which are required for export of diverse cuticular lipids, dimerize in different combinations.

McFarlane HE, Shin JJ, Bird DA, Samuels AL
2010, The Plant cell, 22, 3066-75, 20870961

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2008

Analysis of the Golgi apparatus in Arabidopsis seed coat cells during polarized secretion of pectin-rich mucilage.

Young RE, McFarlane HE, Hahn MG, Western TL, Haughn GW, Samuels AL
2008, The Plant cell, 20, 1623-38, 18523060

Cortical microtubules mark the mucilage secretion domain of the plasma membrane in Arabidopsis seed coat cells.

McFarlane HE, Young RE, Wasteneys GO, Samuels AL
2008, Planta, 227, 1363-75, 18309515

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