Ho-Sung Rhee
Associate Professor
Campus
UTM
CSB Appointment
Full
Research Areas
Animal Biology, Bioinformatics / Computational Biology, Cell Biology, Developmental Biology, Genetics / Genomics, Molecular Biology, Neurobiology, Systems Biology
Education
Ph.D. Pennsylvania State University 2011
M.Sc. Seoul National University 2006
B.Sc. Sogang University 2004
Titles and Honors
Howard Hughes Medical Institute Postdoctoral Fellow 2017
Connaught Scholar 2020
Member, CIHR College of Reviewers
Primary Undergraduate Department
Biology, UTM
Graduate Programs
Cell & Systems Biology
Neuroscience
Developmental Biology
Research Description
How are genes expressed from DNA to make a neuron? How does misregulation of neuronal genes cause neurological diseases? We aim to understand how a neuron is specified and maintained during mammalian development. The acquisition of cell fate is driven by a cell-type-specific gene expression program activated by DNA-binding proteins such as transcription factors. Nearly every gene is packaged into chromatin, which is a complex of DNA and protein in the nucleus of the cell. The recruitment of transcription factors to their target genes is accompanied by dynamic chromatin regulation. We study mammalian gene regulation for more efficient stem cell differentiation methods to make specific types of neurons. To understand these processes, we use stem cell differentiation and utilize molecular, cellular, and epigenetics approaches. By using our cutting-edge approaches, we are studying how neuronal transcription factors control gene expression during neural development. Our expertise in these comprehensive approaches provides greater detail and insight into epigenetic mechanisms of cell type-specific gene expression programs in the mammalian central nervous system. We are now exploring whether this integration of multiple approaches is instrumental in identifying predictive rules for the progression and treatment of neurodegenerative diseases.
Contact Information
Office Phone: 905-569-4931
Office: DV3045
Lab: DV1083
Email
Mailing Address
Department of Biology
University of Toronto
3359 Mississauga Road
Mississauga, ON L5L 1C6
Canada
Publications
2023
Crosstalk between chromatin, chromosomes, and epigenetics
Ialongo A, Yeh S, Rhee HS
2023, Biochemistry and Cell Biology, 10.1139/bcb-2023-0165
2022
The ChIP-Exo Method to Identify Genomic Locations of DNA-Binding Proteins at Near Single Base-Pair Resolution
Yeh S, Rhee HS
2022, Methods in Molecular Biology, 10.1007/978-1-0716-2847-8_4
Extended intergenic DNA contributes to neuron-specific expression of neighboring genes in the mammalian nervous system
Jaura R, Yeh S, Montanera KN, Ialongo A, Anwar Z, Lu Y, Puwakdandawa K, Rhee HS
2022, Nature Communications, 10.1038/s41467-022-30192-z
2020
ChIP-exo: A method to study chromatin structure and organization at near-nucleotide resolution
Montanera KN, Anwar Z, Shibin SM, Rhee HS
2020, , 10.1016/B978-0-12-819414-0.00016-1
High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
Montanera KN, Rhee HS
2020, Journal of Visualized Experiments, 10.3791/61124
ChIP-exo: A method to study chromatin structure and organization at near-nucleotide resolution
Montanera KN, Anwar Z, Shibin SM, Rhee HS
2020, , 10.1016/B978-0-12-819414-0.00016-1
2016
Expression of Terminal Effector Genes in Mammalian Neurons Is Maintained by a Dynamic Relay of Transient Enhancers
Rhee HS, Closser M, Guo Y, Bashkirova EV, Tan GC, Gifford DK, Wichterle H
2016, Neuron, 10.1016/j.neuron.2016.11.037
2014
Subnucleosomal Structures and Nucleosome Asymmetry across a Genome
Rhee H, Bataille A, Zhang L, Pugh B
2014, Cell, 10.1016/j.cell.2014.10.054
A Comprehensive and High-Resolution Genome-wide Response of p53 to Stress
Chang G, Chen X, Park B, Rhee H, Li P, Han K, Mishra T, Chan-Salis K, Li Y, Hardison R, Wang Y, Pugh B
2014, Cell Reports, 10.1016/j.celrep.2014.06.030
2013
Kinetic Competition between Elongation Rate and Binding of NELF Controls Promoter-Proximal Pausing
Li J, Liu Y, Rhee H, Ghosh S, Bai L, Pugh B, Gilmour D
2013, Molecular Cell, 10.1016/j.molcel.2013.05.016
2012
Genome-wide structure and organization of eukaryotic pre-initiation complexes
Rhee HS, Pugh BF
2012, Nature, 10.1038/nature10799
ChIP‐exo Method for Identifying Genomic Location of DNA‐Binding Proteins with Near‐Single‐Nucleotide Accuracy
Rhee HS, Pugh BF
2012, Current Protocols in Molecular Biology, 10.1002/0471142727.mb2124s100
2011
Comprehensive Genome-wide Protein-DNA Interactions Detected at Single-Nucleotide Resolution
Rhee H, Pugh B
2011, Cell, 10.1016/j.cell.2011.11.013
2009
Interaction of Transcriptional Regulators with Specific Nucleosomes across the Saccharomyces Genome
Koerber RT, Rhee HS, Jiang C, Pugh BF
2009, Molecular Cell, 10.1016/j.molcel.2009.09.011
2005
Fatty acids, inhibitors for the DNA binding of c-Myc/Max dimer, suppress proliferation and induce apoptosis of differentiated HL-60 human leukemia cell
Jung KC, Park CH, Hwang YH, Rhee HS, Lee JH, Kim H, Yang CH
2005, Leukemia, 10.1038/sj.leu.2404022
Momordin I, an inhibitor of AP-1, suppressed osteoclastogenesis through inhibition of NF-κB and AP-1 and also reduced osteoclast activity and survival
Hwang YH, Lee JW, Hahm E, Jung KC, Lee JH, Park CH, Rhee HS, Ryu JM, Kim H, Yang C
2005, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2005.09.113
Negative regulation of β-catenin/Tcf signaling by naringenin in AGS gastric cancer cell
Lee JH, Park CH, Jung KC, Rhee HS, Yang CH
2005, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2005.07.146
Ionomycin downregulates β-catenin/Tcf signaling in colon cancer cell line
Park CH, Hahm ER, Lee JH, Jung KC, Rhee HS, Yang CH
2005, Carcinogenesis, 10.1093/carcin/bgi145