Professor Keiko Yoshioka

Keiko Yoshioka



St. George (downtown)

CSB Appointment


Research Areas

Genetics / Genomics, Molecular Biology, Pathology, Plant Biology, Structural Biology, Systems Biology


Ph.D. Hokkaido University 1994
M.Sc. Hokkaido University 1991
B.A. Hokkaido University 1989

Primary Undergraduate Department

Cell & Systems Biology

Graduate Programs

Cell & Systems Biology

Research Description

Understanding the molecular mechanism of plant defense systems is critical in determining how plants defend themselves against various stresses, including pathogens and applying this knowledge to improve agricultural production and protect environment against possible negative impact from pesticides and fungicides. The long-term goal of my laboratory is to understand how plants transmit the intra- and inter-cellular signals of various environmental stresses including pathogen infection. To achieve this goal, we are using both forward and reverse genetics approaches to identifying novel components (genes/proteins) that are involved in plant immunity. One group of genes that we have identified are cyclic nucleotide-gated ion channels (CNGCs). They form a large gene family and affect multiple aspects of plant physiology such as pollen development, thermal sensing and immunity, likely through Ca2+ signaling. Using molecular, genetic and biochemical analyses, we are investigating the molecular basis of CNGCs. Other aspects we focus on are environmental effects on plant immunity. It has been known that environmental condition such as temperature and humidity significantly affect the activation of defense responses. This suggests that plants possess an elaborate signaling network to fine-tune the responses against multiple stresses. We investigate the signal crosstalk between abiotic and biotic stress signal transduction to understand the complexity of the signaling network and apply this knowledge for developing novel technology to protect plants and environment.

Contact Information

Office Phone: 416-978-3545
Office: ESC3070
Lab: ESC3067
Lab Phone: 416-946-0444

Mailing Address

Department of Cell & Systems Biology
University of Toronto
25 Willcocks St.
Toronto, ON M5S 3B2

Visit lab’s website

Recent News

Undergraduate researchers reveal exciting project results at year-end poster session to earn F Michael Barrett Award

On Friday, April 5th, 2024 students from the CSB497, 498 and 499 independent research programs presented their project results in a poster session…

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CSPB awards for CSB’s dedicated plant scientists

CSB students won multiple awards at the annual regional meeting of the Canadian Society for Plant Biology, held at University of Toronto Scarborough…

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CSB Graduate Wins Award for Best Plant Biology Paper of 2021

The 2021 Ragai Ibrahim Award for best student paper in plant biology was awarded to CSB graduate Dr Purva Karia by the Canadian Society for Plant…

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Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots

Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack MK, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J, Costa A, Vanneste S, Napier R
2022, Journal of Experimental Botany, 10.1093/jxb/erac019

Arabidopsis thaliana CYCLIC NUCLEOTIDE‐GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis

Wang L, Ning Y, Sun J, Wilkins KA, Matthus E, McNelly RE, Dark A, Rubio L, Moeder W, Yoshioka K, Véry A, Stacey G, Leblanc‐Fournier N, Legué V, Moulia B, Davies JM
2022, New Phytologist, 10.1111/nph.17987

A tale of many families: calcium channels in plant immunity

Xu G, Moeder W, Yoshioka K, Shan L
2022, The Plant Cell, 10.1093/plcell/koac033

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A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants

Wang Y, Gong Q, Wu Y, Huang F, Ismayil A, Zhang D, Li H, Gu H, Ludman M, Fátyol K, Qi Y, Yoshioka K, Hanley-Bowdoin L, Hong Y, Liu Y
2021, Cell Host & Microbe, 10.1016/j.chom.2021.07.003

CYCLIC NUCLEOTIDE-GATED ION CHANNEL 2 modulates auxin homeostasis and signaling

Chakraborty S, Toyota M, Moeder W, Chin K, Fortuna A, Champigny M, Vanneste S, Gilroy S, Beeckman T, Nambara E, Yoshioka K
2021, Plant Physiology, 10.1093/plphys/kiab332

Multiple phosphorylation events of the mitochondrial membrane protein, TTM1, regulate cell death during senescence

Karia P, Yoshioka K, Moeder W
2021, The Plant Journal, 10.1111/tpj.15470

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Plant Cyclic Nucleotide-Gated Channels: New Insights on Their Functions and Regulation

Dietrich P, Moeder W, Yoshioka K
2020, Plant Physiology, 10.1104/pp.20.00425

Calcium channel in plants helps shut the door on intruders

Yoshioka K, Moeder W
2020, Nature, 10.1038/d41586-020-02504-0

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A host–pathogen interactome uncovers phytopathogenic strategies to manipulate plant ABA responses

Cao FY, Khan M, Taniguchi M, Mirmiran A, Moeder W, Lumba S, Yoshioka K, Desveaux D
2019, The Plant Journal, 10.1111/tpj.14425

The Receptor Kinases BAK1/SERK4 Regulate Ca2+ Channel-Mediated Cellular Homeostasis for Cell Death Containment

Yu X, Xu G, Li B, de Souza Vespoli L, Liu H, Moeder W, Chen S, de Oliveira MV, Ariádina de Souza S, Shao W, Rodrigues B, Ma Y, Chhajed S, Xue S, Berkowitz GA, Yoshioka K, He P, Shan L
2019, Current Biology, 10.1016/j.cub.2019.09.018

Ca2+ to the rescue – Ca2+channels and signaling in plant immunity

Moeder W, Phan V, Yoshioka K
2019, Plant Science, 10.1016/j.plantsci.2018.04.012

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Arabidopsis ETHYLENE RESPONSE FACTOR 8 (ERF8) has dual functions in ABA signaling and immunity

Cao FY, DeFalco TA, Moeder W, Li B, Gong Y, Liu X, Taniguchi M, Lumba S, Toh S, Shan L, Ellis B, Desveaux D, Yoshioka K
2018, BMC Plant Biology, 10.1186/s12870-018-1402-6

A novel role for Cyclic Nucleotide-Gated Ion Channel 2 (DND1) in auxin signaling

Chakraborty S, Toyota M, Moeder W, Chin K, Fortuna A, Champigny M, Vanneste S, Gilroy S, Beeckman T, Yoshioka K
2018, , 10.1101/508572

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CNGCs break through-A rice cyclic nucleotide-gated channel paves the way for pollen tube growth.

Moeder W, Yoshioka K
2017, PLoS genetics, 13, e1007066, 29190294

Translatome analysis of an NB-LRR immune response identifies important contributors to plant immunity in Arabidopsis

Meteignier L, El Oirdi M, Cohen M, Barff T, Matteau D, Lucier J, Rodrigue S, Jacques P, Yoshioka K, Moffett P
2017, Journal of Experimental Botany, 10.1093/jxb/erx078

Calmodulin as a Ca2+-Sensing Subunit of Arabidopsis Cyclic Nucleotide-Gated Channel Complexes

Fischer C, DeFalco TA, Karia P, Snedden WA, Moeder W, Yoshioka K, Dietrich P
2017, Plant and Cell Physiology, 10.1093/pcp/pcx052

Using GCaMP3 to Study Ca2+ Signaling in Nicotiana Species

DeFalco TA, Toyota M, Phan V, Karia P, Moeder W, Gilroy S, Yoshioka K
2017, Plant and Cell Physiology, doi:10.1093/pcp/pcx053

Triphosphate Tunnel Metalloenzyme Function in Senescence Highlights a Biological Diversification of This Protein Superfamily

Ung H, Karia P, Ebine K, Ueda T, Yoshioka K, Moeder W
2017, Plant Physiology, 10.1104/pp.17.00700

Plant Immunity

Chakraborty S, Moeder W, Yoshioka K
2017, , 10.1016/B978-0-12-809633-8.12154-5

Microbial diversity in leaves, trunk and rhizosphere of coconut palms (Cocos nucifera L.) associated with the coconut lethal yellowing phytoplasma in Grand-Lahou, Cte dIvoire

Nadia PM, Ahmed H, Henry ST, Tacra TL, Julia C, Pauline W, Hortense AD, Jean LKK, Keiko Y, Wolfgang M, James S, Yaima AR
2017, African Journal of Biotechnology, 10.5897/AJB2017.16006

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Opening the Gates: Insights into Cyclic Nucleotide-Gated Channel-Mediated Signaling.

DeFalco TA, Moeder W, Yoshioka K
2016, Trends in plant science, 21, 903-906, 27623305

Multiple Calmodulin-binding Sites Positively and Negatively Regulate Arabidopsis CYCLIC NUCLEOTIDE-GATED CHANNEL12

DeFalco TA, Marshall CB, Munro K, Kang H, Moeder W, Ikura M, Snedden WA, Yoshioka K
2016, The Plant Cell, 10.1105/tpc.15.00870

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Arabidopsis triphosphate tunnel metalloenzyme2 is a negative regulator of the salicylic Acid-mediated feedback amplification loop for defense responses

Ung H, Moeder W, Yoshioka K
2014, Plant physiology, 166, 1009-21, 25185123

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Crystal structure and biochemical analyses reveal that the Arabidopsis triphosphate tunnel metalloenzyme AtTTM3 is a tripolyphosphatase involved in root development

Moeder W, Garcia-Petit C, Ung H, Fucile G, Samuel MA, Christendat D, Yoshioka K
2013, The Plant journal : for cell and molecular biology, 76, 615-26, 24004165

Expression and gene knockdown of zebrafish Ca(2+)/calmodulin-dependent protein kinase Iδ-LL

Senga Y, Yoshioka K, Kameshita I, Sueyoshi N
2013, Archives of biochemistry and biophysics, 540, 41-52, 24099663

The Arabidopsis cyclic nucleotide-gated ion channels AtCNGC2 and AtCNGC4 work in the same signaling pathway to regulate pathogen defense and floral transition

Chin K, DeFalco TA, Moeder W, Yoshioka K
2013, Plant physiology, 163, 611-24, 24027242

A suppressor screen of the chimeric AtCNGC11/12 reveals residues important for intersubunit interactions of cyclic nucleotide-gated ion channels

Abdel-Hamid H, Chin K, Moeder W, Shahinas D, Gupta D, Yoshioka K
2013, Plant physiology, 162, 1681-93, 23735507

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Texture, sensory and swallowing characteristics of high-pressure-heat-treated pork meat gel as a dysphagia diet

Tokifuji A, Matsushima Y, Hachisuka K, Yoshioka K
2013, Meat science, 93, 843-8, 23313970

Potential inaccuracies in chloride measurements in patients with severe metabolic acidosis

Makiishi T, Nishimura N, Yoshioka K, Yamamoto S, Mitsuhashi R, Maeda S, Konishi T, Hirose K
2012, International journal of nephrology, 2012, 768316, 22778953

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High throughput chemical screening supports the involvement of Ca2+ in cyclic nucleotide-gated ion channel-mediated programmed cell death in Arabidopsis

Abdel-Hamid H, Chin K, Moeder W, Yoshioka K
2011, Plant signaling & behavior, 6, 1817-9, 22041991

Altered germination and subcellular localization patterns for PUB44/SAUL1 in response to stress and phytohormone treatments

Salt JN, Yoshioka K, Moeder W, Goring DR
2011, PloS one, 6, e21321, 21738636

The role of cyclic nucleotide-gated ion channels in plant immunity

Moeder W, Urquhart W, Ung H, Yoshioka K
2011, Molecular plant, 4, 442-52, 21459831

The cyclic nucleotide-gated channels AtCNGC11 and 12 are involved in multiple Ca²⁺-dependent physiological responses and act in a synergistic manner

Urquhart W, Chin K, Ung H, Moeder W, Yoshioka K
2011, Journal of experimental botany, 62, 3671-82, 21414958

The roles of ABA in plant-pathogen interactions

Cao FY, Yoshioka K, Desveaux D
2011, Journal of plant research, 124, 489-99, 21380629

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SA-ABA antagonism in defense responses

Moeder W, Ung H, Mosher S, Yoshioka K
2010, Plant signaling & behavior, 5, 1231-3, 20861686

Calmodulin binding to Arabidopsis cyclic nucleotide gated ion channels

Abdel-Hamid H, Chin K, Shahinas D, Moeder W, Yoshioka K
2010, Plant signaling & behavior, 5, 1147-9, 21150265

Leaf senescence signaling: the Ca2+-conducting Arabidopsis cyclic nucleotide gated channel2 acts through nitric oxide to repress senescence programming

Ma W, Smigel A, Walker RK, Moeder W, Yoshioka K, Berkowitz GA
2010, Plant physiology, 154, 733-43, 20699402

Importance of the alphaC-helix in the cyclic nucleotide binding domain for the stable channel regulation and function of cyclic nucleotide gated ion channels in Arabidopsis

Chin K, Moeder W, Abdel-Hamid H, Shahinas D, Gupta D, Yoshioka K
2010, Journal of experimental botany, 61, 2383-93, 20378667

The lesion-mimic mutant cpr22 shows alterations in abscisic acid signaling and abscisic acid insensitivity in a salicylic acid-dependent manner

Mosher S, Moeder W, Nishimura N, Jikumaru Y, Joo SH, Urquhart W, Klessig DF, Kim SK, Nambara E, Yoshioka K
2010, Plant physiology, 152, 1901-13, 20164209

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Forward and reverse genetics to identify genes involved in the age-related resistance response in Arabidopsis thaliana

Carviel JL, Al-Daoud F, Neumann M, Mohammad A, Provart NJ, Moeder W, Yoshioka K, Cameron RK
2009, Molecular plant pathology, 10, 621-34, 19694953

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Lesion mimic mutants: A classical, yet still fundamental approach to study programmed cell death

Moeder W, Yoshioka K
2008, Plant signaling & behavior, 3, 764-7, 19513227

Identification of a functionally essential amino acid for Arabidopsis cyclic nucleotide gated ion channels using the chimeric AtCNGC11/12 gene

Baxter J, Moeder W, Urquhart W, Shahinas D, Chin K, Christendat D, Kang HG, Angelova M, Kato N, Yoshioka K
2008, The Plant journal : for cell and molecular biology, 56, 457-69, 18643993

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Cyclic nucleotide gated channels and ca-mediated signal transduction during plant innate immune response to pathogens

Ma W, Yoshioka K, Berkowitz GA
2007, Plant signaling & behavior, 2, 548-50, 19704555

The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca2+ dependent manner

Urquhart W, Gunawardena AH, Moeder W, Ali R, Berkowitz GA, Yoshioka K
2007, Plant molecular biology, 65, 747-61, 17885810

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NPS6, encoding a nonribosomal peptide synthetase involved in siderophore-mediated iron metabolism, is a conserved virulence determinant of plant pathogenic ascomycetes

Oide S, Moeder W, Krasnoff S, Gibson D, Haas H, Yoshioka K, Turgeon BG
2006, The Plant cell, 18, 2836-53, 17056706

The chimeric Arabidopsis CYCLIC NUCLEOTIDE-GATED ION CHANNEL11/12 activates multiple pathogen resistance responses

Yoshioka K, Moeder W, Kang HG, Kachroo P, Masmoudi K, Berkowitz G, Klessig DF
2006, The Plant cell, 18, 747-63, 16461580

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Involvement of the small GTPase Rac in the defense responses of tobacco to pathogens

Moeder W, Yoshioka K, Klessig DF
2005, Molecular plant-microbe interactions : MPMI, 18, 116-24, 15720080

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High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression

Zhou F, Menke FL, Yoshioka K, Moder W, Shirano Y, Klessig DF
2004, The Plant journal : for cell and molecular biology, 39, 920-32, 15341634

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