Professor Leslie Buck

Leslie T. Buck

Professor


Campus

St. George (downtown)

CSB Appointment

Full

Research Areas

Animal Biology, Cell Biology, Neurobiology, Physiology

Education

Ph.D. University of British Columbia 1993
B.Sc.. Brock University 1986

Primary Undergraduate Department

Cell & Systems Biology

Graduate Programs

Cell & Systems Biology
Collaborative Program in Neuroscience

Academic or Administrative Appointments

Associate Chair for Graduate Studies – July 1, 2009 to July 1, 2015

Research Description

We use a comparative neurobiological/physiological/molecular approach to understand how animals survive environmental extremes, in particular how painted turtles and goldfish survive without oxygen for weeks.


Contact Information

Office Phone: 416-978-3506
Office: RW 329B
Lab: RW328/RW329
Lab Phone: 416-946-7238
Email

Mailing Address

Department of Cell & Systems Biology
University of Toronto
25 Harbord St.
Toronto, ON M5S 3G5
Canada


Publications

2019

Mitochondrial matrix pH acidifies during anoxia and is maintained by the FF-ATPase in anoxia-tolerant painted turtle cortical neurons.

Hawrysh PJ, Buck LT
2019, FEBS open bio, 9, 571-581, 30984533

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2018

Taurine activates glycine and GABA receptor currents in anoxia-tolerant painted turtle pyramidal neurons.

Miles AR, Hawrysh PJ, Hossein-Javaheri N, Buck LT
2018, The Journal of experimental biology, 221, 30237241

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2016

Stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and GABA receptor inhibition.

Hossein-Javaheri N, Wilkie MP, Lado WE, Buck LT
2017, The Journal of experimental biology, 220, 695-704, 27923876

Assessment of anoxia tolerance and photoperiod dependence of GABAergic polarity in the pond snail Lymnaea stagnalis.

Buck LT, Bond HC, Malik A
2017, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 203, 193-200, 27664385

Phosphorylation of the mitochondrial ATP-sensitive potassium channel occurs independently of PKCε in turtle brain.

Hawrysh PJ, Miles AR, Buck LT
2016, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 200, 44-53, 27280321

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2015

Decreases in mitochondrial reactive oxygen species initiate GABAA receptor-mediated electrical suppression in anoxia-tolerant turtle neurons

Hogg DW, Pamenter ME, Dukoff DJ, Buck LT
2015, The Journal of physiology, 25781154

Proteomic changes in the brain of the western painted turtle (Chrysemys picta bellii) during exposure to anoxia

Smith RW, Cash P, Hogg DW, Buck LT
2015, Proteomics, 25583675

Transcriptomic Responses of the Heart and Brain to Anoxia in the Western Painted Turtle.

Keenan SW, Hill CA, Kandoth C, Buck LT, Warren DE
2015, PloS one, 10, e0131669, 26147940

Sensing and surviving hypoxia in vertebrates.

Jonz MG, Buck LT, Perry SF, Schwerte T, Zaccone G
2016, Annals of the New York Academy of Sciences, 1365, 43-58, 25959851

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2014

Scavenging ROS dramatically increases NMDA receptor whole cell currents in painted turtle cortical neurons

Dukoff DJ, Hogg DW, Hawrsyh PJ, Buck LT
2014, The Journal of experimental biology, 25063855

Environmental remodelling of GABAergic and glutamatergic neurotransmission: rise of the anoxia-tolerant turtle brain

Hogg DW, Hawrysh PJ, Buck LT
2014, Journal of thermal biology, 44, 85-92, 25086978

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2013

Anoxia-mediated calcium release through the mitochondrial permeability transition pore silences NMDA receptor currents in turtle neurons

Hawrysh PJ, Buck LT
2013, The Journal of experimental biology, 216, 4375-87, 24259257

The western painted turtle genome, a model for the evolution of extreme physiological adaptations in a slowly evolving lineage

Bradley Shaffer H, Minx P, Warren DE, Shedlock AM, Thomson RC, Valenzuela N, Abramyan J, Amemiya CT, Badenhorst D, Biggar KK, Borchert GM, Botka CW, Bowden RM, Braun EL, Bronikowski AM, Bruneau BG, Buck LT, Capel B, Castoe TA, Czerwinski M, Delehaunty KD, Edwards SV, Fronick CC, Fujita MK, Fulton L, Graves TA, Green RE, Haerty W, Hariharan R, Hernandez O, Hillier LW, Holloway AK, Janes D, Janzen FJ, Kandoth C, Kong L, de Koning AJ, Li Y, Literman R, McGaugh SE, Mork L, O'Laughlin M, Paitz RT, Pollock DD, Ponting CP, Radhakrishnan S, Raney BJ, Richman JM, St John J, Schwartz T, Sethuraman A, Spinks PQ, Storey KB, Thane N, Vinar T, Zimmerman LM, Warren WC, Mardis ER, Wilson RK
2013, Genome biology, 14, R28, 23537068

Oxygen-sensitive reduction in Ca²⁺-activated K⁺ channel open probability in turtle cerebrocortex

Rodgers-Garlick CI, Hogg DW, Buck LT
2013, Neuroscience, 237, 243-54, 23384611

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2012

Painted turtle cortex is resistant to an in vitro mimic of the ischemic mammalian penumbra

Pamenter ME, Hogg DW, Gu XQ, Buck LT, Haddad GG
2012, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 32, 2033-43, 22805876

Oxygen sensitive synaptic neurotransmission in anoxia-tolerant turtle cerebrocortex

Buck LT, Hogg DW, Rodgers-Garlick C, Pamenter ME
2012, Advances in experimental medicine and biology, 758, 71-9, 23080145

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2011

The relationship between NMDA receptor function and the high ammonia tolerance of anoxia-tolerant goldfish

Wilkie MP, Pamenter ME, Duquette S, Dhiyebi H, Sangha N, Skelton G, Smith MD, Buck LT
2011, The Journal of experimental biology, 214, 4107-20, 22116753

Endogenous GABA(A) and GABA(B) receptor-mediated electrical suppression is critical to neuronal anoxia tolerance

Pamenter ME, Hogg DW, Ormond J, Shin DS, Woodin MA, Buck LT
2011, Proceedings of the National Academy of Sciences of the United States of America, 108, 11274-9, 21690381

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2010

Regulation of AMPA receptor currents by mitochondrial ATP-sensitive K+ channels in anoxic turtle neurons

Zivkovic G, Buck LT
2010, Journal of neurophysiology, 104, 1913-22, 20685922

Adenosinergic modulation of neuronal activity in the pond snail Lymnaea stagnalis

Malik A, Buck LT
2010, The Journal of experimental biology, 213, 1126-32, 20228349

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2009

Matching cellular metabolic supply and demand in energy-stressed animals

Staples JF, Buck LT
2009, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 153, 95-105, 19535026

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2008

delta-Opioid receptor antagonism induces NMDA receptor-dependent excitotoxicity in anoxic turtle cortex

Pamenter ME, Buck LT
2008, The Journal of experimental biology, 211, 3512-7, 18931323

Evidence of anoxia-induced channel arrest in the brain of the goldfish (Carassius auratus)

Wilkie MP, Pamenter ME, Alkabie S, Carapic D, Shin DS, Buck LT
2008, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 148, 355-62, 18620076

Endogenous reductions in N-methyl-d-aspartate receptor activity inhibit nitric oxide production in the anoxic freshwater turtle cortex

Pamenter ME, Hogg DW, Buck LT
2008, FEBS letters, 582, 1738-42, 18466771

Neuronal membrane potential is mildly depolarized in the anoxic turtle cortex

Pamenter ME, Buck LT
2008, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 150, 410-4, 18519169

Adenosine A1 receptor activation mediates NMDA receptor activity in a pertussis toxin-sensitive manner during normoxia but not anoxia in turtle cortical neurons

Pamenter ME, Shin DS, Buck LT
2008, Brain research, 1213, 27-34, 18455705

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2007

Mitochondrial ATP-sensitive K+ channels regulate NMDAR activity in the cortex of the anoxic western painted turtle

Pamenter ME, Shin DS, Cooray M, Buck LT
2008, The Journal of physiology, 586, 1043-58, 18079161

AMPA receptors undergo channel arrest in the anoxic turtle cortex

Pamenter ME, Shin DS, Buck LT
2008, American journal of physiology. Regulatory, integrative and comparative physiology, 294, R606-13, 18056983

Anoxia-induced changes in reactive oxygen species and cyclic nucleotides in the painted turtle

Pamenter ME, Richards MD, Buck LT
2007, Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 177, 473-81, 17347830

Hypoxia tolerance in reptiles, amphibians, and fishes: life with variable oxygen availability

Bickler PE, Buck LT
2007, Annual review of physiology, 69, 145-70, 17037980

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2006

Excitatory actions of GABA mediate severe-hypoxia-induced depression of neuronal activity in the pond snail (Lymnaea stagnalis)

Cheung U, Moghaddasi M, Hall HL, Smith JJ, Buck LT, Woodin MA
2006, The Journal of experimental biology, 209, 4429-35, 17079713

Piscine insights into comparisons of anoxia tolerance, ammonia toxicity, stroke and hepatic encephalopathy

Walsh PJ, Veauvy CM, McDonald MD, Pamenter ME, Buck LT, Wilkie MP
2007, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 147, 332-43, 17046301

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2005

Calcium and protein phosphatase 1/2A attenuate N-methyl-D-aspartate receptor activity in the anoxic turtle cortex

Shin DS, Wilkie MP, Pamenter ME, Buck LT
2005, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 142, 50-7, 16139540

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2004

Adenosine as a signal for ion channel arrest in anoxia-tolerant organisms

Buck LT
2004, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 139, 401-14, 15544964

Time-dependent expression of heat shock proteins 70 and 90 in tissues of the anoxic western painted turtle

Ramaglia V, Buck LT
2004, The Journal of experimental biology, 207, 3775-84, 15371485

Bacterial infection and tissue-specific Hsp72, -73 and -90 expression in western painted turtles

Ramaglia V, Harapa GM, White N, Buck LT
2004, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 138, 139-48, 15450861

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2003

Gap junctions do not underlie changes in whole-cell conductance in anoxic turtle brain

Shin DS, Ghai H, Cain SW, Buck LT
2003, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 134, 181-94, 12507621

Effect of anoxia and pharmacological anoxia on whole-cell NMDA receptor currents in cortical neurons from the western painted turtle

Shin DS, Buck LT
2003, Physiological and biochemical zoology : PBZ, 76, 41-51, 12695985

Tissue-specific expression of inducible and constitutive Hsp70 isoforms in the western painted turtle

Scott MA, Locke M, Buck LT
2003, The Journal of experimental biology, 206, 303-11, 12477900

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2002

Molecular adaptations for survival during anoxia: lessons from lower vertebrates

Bickler PE, Donohoe PH, Buck LT
2002, The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry, 8, 234-42, 12061503

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