John Calarco

 

John Calarco website Academic Title: Assistant Professor

Campus: St. George

CSB Appointment: Full

Primary Undergraduate Department:
Cell & Systems Biology

Graduate Programs:
Cell & Systems Biology

Titles and Honors:
Medicine by Design Investigator

Canada Research Chair (Tier II) in Neuronal RNA Biology

Academic or Administrative Appointments:
Education:
Ph.D. University of Toronto, 2011
B.Sc. University of Toronto, 2005

 

Mailing Address
Department of Cell & Systems Biology
University of Toronto
25 Harbord St.
Toronto, ON M5S 3G5
Canada

 

Contact Information
Office phone: 416-978-5766
Office: 513A
Lab: 511
Lab phone: 416-946-7785
Email: john.calarco@utoronto.ca
URL: www.calarcolab.ca

 

Research Areas
Animal Biology
Bioinformatics & Computational Biology
Developmental Biology
Functional Genomics
Genetics
Molecular Biology
Neurobiology

 

Research
 A major goal of our lab is to better understand the role of post-transcriptional gene regulation in the development, differentiation, and function of the nervous system. The nervous system provides an attractive model to study the mechanism and role of cell-type specific gene regulation because it is composed of diverse neuronal cells that require customized regulatory programs to achieve specialized functions. One research area in my group will focus on understanding the mechanisms and function of alternative splicing in the developing and mature nervous system using both mammalian stem cells and C. elegans. Through the process of alternative splicing, multiple messenger RNAs can be generated from single precursor transcripts. The evolution of alternative splicing has greatly contributed to the diversification of metazoan transcriptomes, and in some species can generate up to an order of magnitude greater number of transcript isoforms than the corresponding repertoire of protein-coding genes. We pursue these problems using a combination of biochemistry, cell biology, molecular genetics, and genome-wide analyses. Additionally, several ongoing projects are directing our research towards investigating the role of other layers of co- and post-transcriptional gene regulation in nervous system development and function.

 

Publications

2018

Top
Synthetic Genetic Interaction (CRISPR-SGI) Profiling in ..Calarco JA, Norris AD. Bio Protoc 2018 Mar 05; 8(5):
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2017

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An Elongin-Cullin-SOCS Box Complex Regulates Stress-Induced Serotonergic Neuromodulation..Gracida X, Dion MF, Harris G, Zhang Y, Calarco JA. Cell Rep 2017 Dec 12; 21(11):3089-3101
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CRISPR-mediated genetic interaction profiling identifies RNA binding proteins controlling metazoan fitness..Norris AD, Gracida X, Calarco JA. Elife 2017 07 18; 6():
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Cell type-specific transcriptome profiling in C. elegans using the Translating Ribosome Affinity Purification technique..Gracida X, Calarco JA. Methods 2017 08 15; 126():130-137
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Serotonin-dependent kinetics of feeding bursts underlie a graded response to food availability in C. elegans..Lee KS, Iwanir S, Kopito RB, Scholz M, Calarco JA, Biron D, Levine E. Nat Commun 2017 02 01; 8():14221
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2016

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Neuroendocrine modulation sustains the forward motor state..Lim MA, Chitturi J, Laskova V, Meng J, Findeis D, Wiekenberg A, Mulcahy B, Luo L, Li Y, Lu Y, Hung W, Qu Y, Ho CY, Holmyard D, Ji N, McWhirter R, Samuel AD, Miller DM, Schnabel R, Calarco JA, Zhen M. Elife 2016 11 18; 5():
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Regulation of Tissue-Specific Alternative Splicing: C. elegans as a Model System..Gracida X, Norris AD, Calarco JA. Adv. Exp. Med. Biol. 2016; 907():229-61
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2015

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Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System..Calarco JA, Friedland AE. Methods Mol. Biol. 2015; 1327():59-74
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Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes..Norris AD, Kim HM, Colaiácovo MP, Calarco JA. Genetics 2015 Oct; 201(2):449-58
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2014

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EOL-1, the homolog of the mammalian Dom3Z, regulates olfactory learning in C. elegans..Shen Y, Zhang J, Calarco JA, Zhang Y. J. Neurosci. 2014 Oct 01; 34(40):13364-70
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A pair of RNA-binding proteins controls networks of splicing events contributing to specialization of neural cell types..Norris AD, Gao S, Norris ML, Ray D, Ramani AK, Fraser AG, Morris Q, Hughes TR, Zhen M, Calarco JA. Mol. Cell 2014 Jun 19; 54(6):946-59
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2013

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Heritable genome editing in C. elegans via a CRISPR-Cas9 system..Friedland AE, Tzur YB, Esvelt KM, Colaiácovo MP, Church GM, Calarco JA. Nat. Methods 2013 Aug; 10(8):741-3
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\'Cryptic\' exons reveal some of their secrets..Calarco JA. Elife 2013 Jan 22; 2():e00476
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Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR–Cas9 System.Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP. 2013. Genetics.
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2012

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Emerging Roles of Alternative Pre-mRNA Splicing Regulation in Neuronal Development and Function..Norris AD, Calarco JA. Front Neurosci 2012; 6():122
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